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RNA Extraction from Purified Virus Particles

  • Jeanne Dijkstra
  • Cees P. de Jager
Part of the Springer Lab Manual book series (SLM)

Abstract

The extraction procedure involves three consecutive extractions with phenol and three washings of the extracted RNA with 70 % ethanol. Several measures ensure high quality of the RNA:
  • Working under conditions of high pH in which the binding between RNA and coat proteins is weakened

  • Applying macaloid (a clay) to adsorb RNase

  • Use of SDS to denature RNase

  • Adding EDTA for binding oxidising ions, such as Fe3+, which may otherwise generate harmful oxygen radicals

  • Use of redistilled phenol to avoid the presence of oxidised polyphenols

  • Use of chloroform to remove fatty acids

  • Use of isoamyl alcohol to avoid generation of foam due to the presence of SDS

Keywords

Extraction Buffer Pasteur Pipette Microfuge Tube Isoamyl Alcohol Extraction Tube 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • Jeanne Dijkstra
    • 1
  • Cees P. de Jager
    • 1
  1. 1.Department of VirologyWageningen Agricultural UniversityWageningenThe Netherlands

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