Particle Morphology and Size as Determined with the Electron Microscope

  • Jeanne Dijkstra
  • Cees P. de Jager
Part of the Springer Lab Manual book series (SLM)

Abstract

Plant virus particles can be visualised only in an electron microscope (EM). By utilising beams of electrons and electromagnetic lenses, the EM has a very high resolving power, allowing magnifications of at least X 200,000. Specimens are prepared on a small copper grid, covered with a thin support film of carbon-reinforced Formvar. Electrons pass through the object and hit a fluorescent screen, which is lighted up.

Keywords

Catalase Glutaraldehyde Tral Uranyl Molybdate 

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References

  1. Griffin RL (1990) Using the transmission electron microscope in the biological sciences. Ellis Horwood, New YorkGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • Jeanne Dijkstra
    • 1
  • Cees P. de Jager
    • 1
  1. 1.Department of VirologyWageningen Agricultural UniversityWageningenThe Netherlands

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