Abstract
Some viruses cannot easily be eliminated from an infected plant by meristem-tip culture because survival of the explants is very low and/or virus is still present in the excised tips. To overcome this problem, heat treatment is given to the infected plant material prior to excision of the meristem tip. After heat treatment which may inactivate the virus, larger explants may be virus free. However, high temperatures also negatively affect the plant and its meristems, hence preliminary experiments must be conducted to find out the maximum temperature to be applied. Good results have been obtained with the following procedure.
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References
George EF, Puttock DJM, George HJ (eds)(1988) Plant culture media vol. 1 (formulations and use) and vol. 2 (commentary and analysis). Exegetics, Edington, UK
Mellor FC, Stace-Smith R (1977) Virus-free potatoes by tissue culture. In: Reinert J, Bajaj YPS (eds) Applied and fundamental aspects of plant cell, tissue, and organ culture. Springer, Berlin Heidelberg New York, pp 616–635
Murashige T, Skoog F (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant 15: 473–497
Pierik RLM (1987) In vitro culture of higher plants. Martinus Nijhoff, Dordrecht
Quak F (1977) Meristem culture and virus-free plants. In: Reinert J, Bajaj YPS (eds) Applied and fundamental aspects of plant cell, tissue, and organ culture. Springer, Berlin Heidelberg New York, pp 598–615
Van Zaayen A, Van Eijck C, Versluijs JMA (1992) Production of high quality, healthy ornamental crops through meristem culture. Acta Bot Neerl 41: 425–433
Walkey DGA (1991) Applied Plant Virology, 2nd edn. Chapman and Hall, London
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© 1998 Springer-Verlag Berlin Heidelberg
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Dijkstra, J., de Jager, C.P. (1998). Heat Treatment Combined With Meristem-Tip Culture. In: Practical Plant Virology. Springer Lab Manual. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-72030-7_42
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DOI: https://doi.org/10.1007/978-3-642-72030-7_42
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-48981-5
Online ISBN: 978-3-642-72030-7
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