Very good results have been obtained with a large number of viruses using this simple method for preservation, originally developed by McKinney (1953) and modified by Bos (1969). Leaves are cut and dried over calcium chloride (CaCl2). Thereafter, the desiccated leaf material is stored in laboratory test tubes at 0–4°C. Preserved in this way, alfalfa mosaic Alfamovirus, cucumber mosaic Cucumovirus, potato X Potexvirus, potato Y Potyvirus and tobacco ringspot Nepovirus retained their infectivity for periods as long as 15 years, with the first two even for 20 years. Other viruses, such as bean yellow mosaic Potyvirus, beet mosaic Potyvirus, tobacco necrosis Necrovirus, tobacco rattle Tobravirus, tobacco streak Ilarvirus, tomato ringspot Nepovirus and turnip yellow mosaic Tymovirus, remained infective for periods ranging from nearly 3 years to 9 years.
KeywordsLeaf Material Rubber Stopper Cold Room Anhydrous CaCl Nylon Gauze
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