Abstract
Longevity in vitro (LIV) is the length of time after which crude sap from a virus-infected plant loses its infectivity when kept at room temperature (20–22 °C). To determine the LIV of a virus, samples of crude sap are removed from storage at intervals and tested on assay plants. In the absence of information on the stability of a virus in sap, the first series of intervals should be at a geometric progression, e.g. 1, 2, 4, 8, 16, 32...days, until infectivity is lost. As soon as the LIV has been roughly established, the test can be repeated over a narrower range of shorter intervals.
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References
Kurstak E (1981) Handbook of plant virus infections. Comparative diagnosis. Elsevier, Amsterdam
Murant AF, Harrison BD (eds) Descriptions of plant viruses. Commonwealth Mycological Institute, Association of Applied Biologists, Kew, Surrey, England Smith KM (1972) A textbook of plant virus diseases. Longman, London
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© 1998 Springer-Verlag Berlin Heidelberg
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Dijkstra, J., de Jager, C.P. (1998). Determination of the Longevity In Vitro. In: Practical Plant Virology. Springer Lab Manual. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-72030-7_20
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DOI: https://doi.org/10.1007/978-3-642-72030-7_20
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-48981-5
Online ISBN: 978-3-642-72030-7
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