Determination of the Longevity In Vitro
Longevity in vitro (LIV) is the length of time after which crude sap from a virus-infected plant loses its infectivity when kept at room temperature (20–22 °C). To determine the LIV of a virus, samples of crude sap are removed from storage at intervals and tested on assay plants. In the absence of information on the stability of a virus in sap, the first series of intervals should be at a geometric progression, e.g. 1, 2, 4, 8, 16, 32...days, until infectivity is lost. As soon as the LIV has been roughly established, the test can be repeated over a narrower range of shorter intervals.
KeywordsPlant Virus Microfuge Tube Geometric Progression Commonwealth Mycological Institute Standard Inoculum
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