Determination of the Longevity In Vitro

  • Jeanne Dijkstra
  • Cees P. de Jager
Part of the Springer Lab Manual book series (SLM)

Abstract

Longevity in vitro (LIV) is the length of time after which crude sap from a virus-infected plant loses its infectivity when kept at room temperature (20–22 °C). To determine the LIV of a virus, samples of crude sap are removed from storage at intervals and tested on assay plants. In the absence of information on the stability of a virus in sap, the first series of intervals should be at a geometric progression, e.g. 1, 2, 4, 8, 16, 32...days, until infectivity is lost. As soon as the LIV has been roughly established, the test can be repeated over a narrower range of shorter intervals.

Keywords

Penicillin Stopper Neomycin Suspen 

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References

  1. Kurstak E (1981) Handbook of plant virus infections. Comparative diagnosis. Elsevier, AmsterdamGoogle Scholar
  2. Murant AF, Harrison BD (eds) Descriptions of plant viruses. Commonwealth Mycological Institute, Association of Applied Biologists, Kew, Surrey, England Smith KM (1972) A textbook of plant virus diseases. Longman, LondonGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • Jeanne Dijkstra
    • 1
  • Cees P. de Jager
    • 1
  1. 1.Department of VirologyWageningen Agricultural UniversityWageningenThe Netherlands

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