Abstract
The dilution end-point (DEP) is the highest dilution of sap from a virus-infected plant which is still infectious, but is usually given as the range between this dilution and the next one at which the infectivity is lost. In view of the great variation expected to occur in such crude tests, sap dilutions less than tenfold do not make sense. Dilutions are usually on a logarithmic scale and deionised water is used as a diluent. Each dilution is inoculated on a number of assay plants, preferably local-lesion hosts, according to an appropriate experimental design (Part III).
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Author information
Authors and Affiliations
Rights and permissions
Copyright information
© 1998 Springer-Verlag Berlin Heidelberg
About this chapter
Cite this chapter
Dijkstra, J., de Jager, C.P. (1998). Determination of the Dilution End-Point. In: Practical Plant Virology. Springer Lab Manual. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-72030-7_18
Download citation
DOI: https://doi.org/10.1007/978-3-642-72030-7_18
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-48981-5
Online ISBN: 978-3-642-72030-7
eBook Packages: Springer Book Archive