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Flow Cytometry of Leukocyte Alkaline Phosphatase in Human Hematopoietic Cells

  • Conference paper
Acute Leukemias VII

Part of the book series: Haematology and Blood Transfusion / Hämatologie und Bluttransfusion ((HAEMATOLOGY,volume 39))

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Abstract

Leukocyte alkaline phosphatase (LAP) is an enzyme expressed on the external aspect of the neutrophilic granulocyte plasma membrane, and it represents a specific marker for the fully differentiated granulocyte. In this report, we characterize 1B12.1, a monoclonal antibody raised against human bone alkaline phosphatase, for its ability to recognize the LAP protein. With this antibody, we developed a quantitative flow cytometry-based method for the determination of LAP. Double fluorescence flow cytometry demonstrates that the LAP protein is present in relatively high amounts in the neutrophilic granulocytes, but not in monocytes, natural killer cells, B- and T-lymphocytes of normal individuals. The protein is absent in granulocytes obtained from Chronic Myeloid Leukemia and Paroxysmal Nocturnal Hemoglobinuria patients. Higher than normal levels of LAP protein are evident in neutrophilic granulocytes of patients suffering from Polycythemia Vera, Essential Thrombocytemia and Severe Aplastic Anemia. However, the highest amounts of LAP protein are present in the granulocytes of normal individuals treated with G-CSF for the isolation of peripheral blood stem cells.

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Author information

Authors and Affiliations

  1. Divisione di Ematologia Ospedali Riuniti Bergamo, Largo Barozzi, 1, 24100, Bergamo, Italy

    A. Rambaldi, G.-M. Borleri, R. Amaru & T. Barbui

  2. Department of Orthopedic Surgery, School of Medicine, Osaka University, 2-2 Yamada-oka, Suita, 565, Japan

    K. Masuhara

  3. Molecular Biology Unit, Centro Catullo e Daniela Borgomainerio, Istituto di Ricerche Farmacologiche Mario Negri, via Eritrea, 62, 20157, Milano, Italy

    M. Giannì, M. Terao & E. Garattini

Authors
  1. A. Rambaldi
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  2. K. Masuhara
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  3. G.-M. Borleri
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  4. R. Amaru
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  5. M. Giannì
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  6. M. Terao
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  7. T. Barbui
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  8. E. Garattini
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Editor information

Editors and Affiliations

  1. Department of Internal Medicine, University of Göttingen, Robert-Koch-Straße 40, 37075, Göttingen, Germany

    W. Hiddemann  & B. Wörmann  & 

  2. Department of Internal Medicine, USA

    T. Büchner

  3. Department of Pediatrics, University of Münster, Albert-Schweitzer-Straße 33, 48149, Münster, Germany

    J. Ritter  & U. Creutzig  & 

  4. Dept. of Hematology, University of Texas, Anderson Cancer Center, 1515 Holcombe Boulevard, 77030, Houston, Tx., USA

    M. Keating MD PhD  & W. Plunkett MD PhD  & 

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© 1998 Springer-Verlag Berlin Heidelberg

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Rambaldi, A. et al. (1998). Flow Cytometry of Leukocyte Alkaline Phosphatase in Human Hematopoietic Cells. In: Hiddemann, W., et al. Acute Leukemias VII. Haematology and Blood Transfusion / Hämatologie und Bluttransfusion, vol 39. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-71960-8_8

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  • DOI: https://doi.org/10.1007/978-3-642-71960-8_8

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-71962-2

  • Online ISBN: 978-3-642-71960-8

  • eBook Packages: Springer Book Archive

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