Expression of Differentiation Antigens on Blast Cells of Acute Myelogenous Leukemia (AML, M0–M6)

  • I. Abramenko
  • D. Gluzman
  • N. Bilous
  • V. Chromyak
  • L. Sklyarenko
  • S. Andreeva
Conference paper
Part of the Haematology and Blood Transfusion / Hämatologie und Bluttransfusion book series (HAEMATOLOGY, volume 39)


Forty one patients with AML (M01 4 patients, M11 5, M21 7, M41 8, M5a1 16, and M61 one patient) have been studied. Cytochemical reactions (MPO, CAE, AcP, ANAE, NE, PAS) and immunological detection of antigens expression (indirect immunoperoxidase, APAAP methods and, in some cases, flow cytometry) were performed. The definition of typical phenotypical pattern for discrete FAB-variants AML was carried out. Significant heterogeneity of monoblasts was revealed. Cases of AML, M5a with unusual phenotypical features were discovered. Its origin from hierarchy of hematopoietic stem cells is discussed.


acute myelogenous leukemia monoblastic leukemia hematopoietic progenitor cells stem cells antigen expression 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Haase D, Feuring-Buske M, Konemann S, Fonatsch Ch, Troff C, Verbeek AP, Hiddemann W, Wormann B (1995) Evidence for malignant transformation in acute myeloid leukemia at the level of early hematopoietic stem cells by cytogenetic analysis of CD34+ subpopulations. Blood 86: 2906–12PubMedGoogle Scholar
  2. 2.
    Mehrotra B, Goerge TI, Kavanauu K, Avet-Loiseau H, Moore D II, Willman ChL, Slovak ML, Atwater S, Head DR, Pallavicini MG (1995) Cytogenetically aberrant cells in the stem cell compartment (CD34+lin-) in acute myeloid leukemia. Blood 86: 1139–47PubMedGoogle Scholar
  3. 3.
    Civin CI, Strauss LC, Brovall C, Fackler MJ, Schawartz JF, Shaper JH (1984) Antigenic analysis of hematopoiesis. III. A hematopoietic progenitor cell surface antigen defined by a monoclonal antibody raised against KG-la cells. J Immunol 133: 157–65PubMedGoogle Scholar
  4. 4.
    Lu L, Xiao M, Shen R-N, Grigsby S, Broxmeyer HE (1993) Enrichment, characterization, and responsiveness of single primitive CD343+ human umbilical cord blood hematopoietic progenitors with high proliferative and replating potential. Blood 81: 41–8PubMedGoogle Scholar
  5. 5.
    Muench MO, Cupp J, Polakoff J, Roncarolo MG (1994) Expression of CD33,CD38 and HLA-DR on CD34+ human fetal liver progenitors with a high proliferative potential. Blood 83: 3170–81PubMedGoogle Scholar
  6. 6.
    Schlossman SF, Boumsell L, Gilks W, Harlan JM, Kishimoto T, Morimoto C, Ritz J, Shaw S, Silverstein R, Springer T, Tedder TF, Todd RF (1995) Leukocyte Typing V. White cell differentiation antigens. New York: Oxford University Press Inc. 1192 ppGoogle Scholar
  7. 7.
    Louache F, Debili N, Marandin A, Coulombel L, Vainchenker W (1994) Expression of CD4 by human hematopoietic progenitors. Blood 84: 3344–55PubMedGoogle Scholar
  8. 8.
    Cumano A, Paige CJ, Iscove NN, Brady G (1992) Bipotential precursors of B cells and macrophages in murine fetal liver. Nature 356: 612–5PubMedCrossRefGoogle Scholar
  9. 9.
    Barcena A, Muench MO, Galy A, Cupp J, Roncarolo MG, Philips JH, Spits H (1993) Phenotypic and functional analysis of T-cell precursors in the human fetal liver and thymus. CD7 expression in the earliest stages of T- and myeloid development. Blood 82: 3401–15PubMedGoogle Scholar
  10. 10.
    Terstappen LWMM, Huang S, Safford M, Lansdorp P, Loken M (1996) Sequential generation of hematopoietic colonies derived from single nonlineage-committed CD34+CD38--progenitor cells. Blood 77: 1218–27Google Scholar
  11. 11.
    Pontvert-Delucq S, Breton-Gorius J, Schmitt C, Baillou C, Guichard J, Najman A, Lemoine FM (1993) Characterization and functional analysis of adult human bone marrow cell subsets in relation to B-lymphoid development. Blood 82: 417–29PubMedGoogle Scholar
  12. 12.
    Sutherland HJ, Blair A, Zapf RW (1996) Characterization of a hierarchy in human acute myeloid leukemia progenitor cells. Blood 87: 4754–61PubMedGoogle Scholar
  13. 13.
    Bein BJ (1990) Leukemia diagnosis. A guide to the FAB classification. London, NewYork: Power Medical Publishing 115 ppGoogle Scholar
  14. 14.
    Cuneo A, Ferrant A, Michaux JL, Boorgaerts M, Demuynck H, Orshoven A, Criel A, Stul M, Chi PD, Hernandez J, Chatelain B, Doyen Ch, Louwagie A, Castoldi G, Cassiman J-J, Berghe H (1995) Cytogenetic profile of minimally differentiated (FAB MO) acute myeloid leukemia: correlation with clinicobiologic findings. Blood 85: 3688–94PubMedGoogle Scholar
  15. 15.
    Terpsta W, Prins A, Ploemacher RE, Wognum BW, Wagemaker G, Lowenberg B, Wielenga JJ (1996) Long-term leukemia-initiating capacity of a CD34- subpopulation of acute myeloid leukemia. Blood 87: 2187–94Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • I. Abramenko
  • D. Gluzman
  • N. Bilous
  • V. Chromyak
  • L. Sklyarenko
  • S. Andreeva

There are no affiliations available

Personalised recommendations