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Involvement of CD34+ Stem Cells in Malignant Transformation in AML and MDS — Genetic Analysis of Sorted Subpopulations by Classical and Molecular Cytogenetics

  • Conference paper
Acute Leukemias VII

Part of the book series: Haematology and Blood Transfusion / Hämatologie und Bluttransfusion ((HAEMATOLOGY,volume 39))

Abstract

In this study we addressed the question whether hematopoietic stem cells characterized by the expression of CD34 are involved in the leukemogenic process in AML and MDS. For this purpose bone marrow cells were sorted according to their expression of CD34 and coexpression of CD38 or CD117 (stem cell factor receptor = CKIT). The sorted subpopulations were genetically analyzed either by cytogenetics or FISH. Successful cytogenetics of sorted CD34+ subpopulations could be performed in 24/54 pts. included in our study (AML: n = 18, MDS: n = 6). At diagnosis 18 displayed a wide variety of different clonal karyotype abnormalities in the unsorted bone marrow: add(2q); +4; 5q−; 5q− and complex anomalies; t(6;9); 6p−; −7; +8; t(8;21); −12; inv(16); i(17q); t(17;20); −21; i(21q). We analyzed sorted stem cell subpopulations with the immunophenotype CD34+/CD38± in 19 pts., in 5 pts. stem cells with the immunophenotype CD34+/ CD117± were examined. We found genetically abnormal stem cells in every subpopulation (CD34+/CD 38−, CD34+/CD38+, CD34+/CD117−, CD34+/CD117−) in every informative case (abnormal karyotype in the unsorted bone marrow and metaphases/interphase cells available for cytogenetic analysis). In 11 pts. a mosaic of normal and abnormal cells was observed in the sorted stem cell subpopulations. In five pts. secondary, progression-associated anomalies were present in the sorted stem cells. We conclude that in AML and MDS malignant transformation and disease progression occur at the level of immature hematopoietic stem cells independent from the phenotype of the leukemic bulk population and the type of genetic alteration.

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Authors and Affiliations

  1. Dept. of Hematology and Oncology, Georg-August-University, Robert-Koch-Str. 40, 37075, Göttingen, Germany

    D. Haase, M. Feuring-Buske, C. Schoch, C. Schäfer, F. Griesinger, C. Troff, B. Gahn, W. Hiddemann & B. Wörmann

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  1. D. Haase
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  2. M. Feuring-Buske
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  3. C. Schoch
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  4. C. Schäfer
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  6. C. Troff
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  7. B. Gahn
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  8. W. Hiddemann
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Editor information

Editors and Affiliations

  1. Department of Internal Medicine, University of Göttingen, Robert-Koch-Straße 40, 37075, Göttingen, Germany

    W. Hiddemann  & B. Wörmann  & 

  2. Department of Internal Medicine, USA

    T. Büchner

  3. Department of Pediatrics, University of Münster, Albert-Schweitzer-Straße 33, 48149, Münster, Germany

    J. Ritter  & U. Creutzig  & 

  4. Dept. of Hematology, University of Texas, Anderson Cancer Center, 1515 Holcombe Boulevard, 77030, Houston, Tx., USA

    M. Keating MD PhD  & W. Plunkett MD PhD  & 

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© 1998 Springer-Verlag Berlin Heidelberg

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Haase, D. et al. (1998). Involvement of CD34+ Stem Cells in Malignant Transformation in AML and MDS — Genetic Analysis of Sorted Subpopulations by Classical and Molecular Cytogenetics. In: Hiddemann, W., et al. Acute Leukemias VII. Haematology and Blood Transfusion / Hämatologie und Bluttransfusion, vol 39. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-71960-8_3

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  • DOI: https://doi.org/10.1007/978-3-642-71960-8_3

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-71962-2

  • Online ISBN: 978-3-642-71960-8

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