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Monitoring of Minimal Residual Disease in Childhood Acute Lymphoblastic Leukemia — Preliminary Data from a Prospective Study

  • T. Seriu
  • W.-D. Ludwig
  • M. Schrappe
  • D. Erz
  • Y. Stark
  • C. R. Bartram
Conference paper
Part of the Haematology and Blood Transfusion / Hämatologie und Bluttransfusion book series (HAEMATOLOGY, volume 39)

Abstract

Minimal residual disease (MRD) was evaluated in bone marrow samples prospectively obtained from children with acute lymphoblastic leukemia (ALL) during complete remission (CR), using allele-specific markers based on junctional regions generated through the recombination of T-cell receptor δ (TCRδ), TCRγ, immunoglobulin K (IgK) chain or sil-tal genes. Suitable TCRδ, TCRγ, IgK and sil-tal recombinations were identified by Southern blot analysis in 27/29 (93%) T-ALL and 152/174 (87%) precursor-B ALL patients. Two or more independent markers were available in 156/203 (77%) cases. Allele-specific oligonucleotides (ASO) were used either as clonospecific probes or primers and reached a detection level between 10−4 to 10−6 in 192/212 (91%) recombined alleles. Six hundred and forty-one remission samples from 112 cases (20 relapsed, 92 in continuous CR) have been analyzed so far. MRD was demonstrated during a period of 6 months after diagnosis in the vast majority of patients who relapsed during the course of treatment. In contrast, 88% of the patients who remained in continuous CR became PCR-negative within 3 months of treatment. Remarkably, 58% of them achieved PCR-negativity within 1 month. Our data suggest that the MRD status at 3 and 6 months after diagnosis may represent a clinically relevant parameter for a second stratification of childhood ALL.

Keywords

Acute Lymphoblastic Leukemia Minimal Residual Disease Junctional Region Childhood Acute Lymphoblastic Leukemia Continuous Complete Remission 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • T. Seriu
    • 1
  • W.-D. Ludwig
    • 2
  • M. Schrappe
    • 3
  • D. Erz
    • 1
  • Y. Stark
    • 1
  • C. R. Bartram
    • 1
  1. 1.Institute of Human GeneticsUniversity of HeidelbergHeidelbergGermany
  2. 2.Department of Medical Oncology and Applied Molecular Biology, Robert-Rössle Cancer CenterFree University of BerlinGermany
  3. 3.Department of Pediatrics IVHannover Medical SchoolHannoverGermany

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