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Fundamentals of the Application of MS and NMR in the Study of Ganglioside Structure

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Gangliosides and Modulation of Neuronal Functions

Part of the book series: NATO ASI Series ((ASIH,volume 7))

Abstract

The exact description of molecular structure has always been a crucial aspect in the study of the biological and pharmacological phenomena associated with gangliosides ever since their discovery 50 years ago. Whereas most of early work on ganglioside structure was based on more or less degradative chemical methods (1–5), physicochemical methods like mass spectrometry and high resolution nuclear magnetic resonance spectroscopy as a non-destructive method have recently been applied in this field with great success. One major breakthrough was achieved by application of electron impact and chemical ionization mass spectrometry especially of permethylated gangliosides that furnished insight into ceramide constituents and carbohydrate structures of glycosphingolipids with molecular weights up to 3000 a.m.u. (6). This work has been reviewed repeatedly (7,8,9). The further development of ganglioside analysis is intimately connected with the introduction of fast atom bombardment mass spectrometry (FAB-MS) (10,11). With this ‘soft ionization’ technique that allows ionization of polar compounds from a solution in a high boiling liquid in combination with high field magnets and highly sensitive detectors, it became possible to analyse such complex compounds like polysialogangliosides without any previous derivatization (12–16 and references cited there).

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Authors and Affiliations

  1. Institut für Physiologische Chemie, Rheinische Friedrich-Wilhelms-Universität, Nussallee 11, D-5300, Bonn - 1, Germany

    H. Egge & J. Peter-Katalinić

Authors
  1. H. Egge
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  2. J. Peter-Katalinić
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Editors and Affiliations

  1. Zoological Institute, University of Stuttgart-Hohenheim, Garbenstr. 30, 7000, Stuttgart 70, Germany

    Hinrich Rahmann

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© 1987 Springer-Verlag Berlin Heidelberg

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Egge, H., Peter-Katalinić, J. (1987). Fundamentals of the Application of MS and NMR in the Study of Ganglioside Structure. In: Rahmann, H. (eds) Gangliosides and Modulation of Neuronal Functions. NATO ASI Series, vol 7. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-71932-5_5

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  • DOI: https://doi.org/10.1007/978-3-642-71932-5_5

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-71934-9

  • Online ISBN: 978-3-642-71932-5

  • eBook Packages: Springer Book Archive

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