Skip to main content

Determination of Chol-1 Antigen in Human Brain and Neuroblastoma Tumor

  • Conference paper
Gangliosides and Modulation of Neuronal Functions

Part of the book series: NATO ASI Series ((ASIH,volume 7))

  • 105 Accesses

Abstract

Chol-1 antigen has been found to be specific for cholinergic neurons in the electric organ of Torpedo Marmorata and guinea-pig brain (1). The antiserum raised against synaptosomes from electric organ has been shown in mammalian brain to bind to a ganglioside antigen (Chol-1) localized on specifically cholinergic terminals (1,2,3) as shown by immunohistochemical methods and immunotoxicity assay.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Richardson PJ, (1981) Quantification of cholinergic synaptosomes from guinea-pig brain. 37: 258–260

    CAS  Google Scholar 

  2. Jones RT, Walker JH, Richardson PJ, Fox GQ, Whittaker VP (1982) Immunohistochemical localization of cholinergic nerve terminal cells Tissue Res. 218: 355–373.

    Google Scholar 

  3. Richardson PJ, Walker JH, Jones RT, Whittaker VP (1982) Identification of a cholinergic specific antigen chol-1 as a ganglioside J. Neurochem. 38: 1605–1614.

    Article  PubMed  CAS  Google Scholar 

  4. Williams MA, McCluer RH (1980) The use of sep-pak C18 cartridges during the isolation of gangliosides. J. Neurochem. 35: 266–269.

    Article  PubMed  CAS  Google Scholar 

  5. Svennerholm L (1957) Quantitative estimation of sialic acid. Biochem Biophys Acta 24: 604–611.

    Article  PubMed  CAS  Google Scholar 

  6. Phillis E, Richardson PJ (1986) The detection of the cholinergic ganglioside chol-1 in rat brain Biochem Soc Trans 14: 765.

    Google Scholar 

  7. Magnani JL, Nilsson B, Brockhaus M, Zopf D, Steplewski Z., Koprowski H., Ginsburg V (1982) Monoclonal antibody defined antigen associated with gastrointestinal cancer is a ganglioside containing sialylated lactofucopentaose II. J Biol Chem 257: 14365–14369.

    PubMed  CAS  Google Scholar 

  8. Harpin ML, Coulon-Morelec MJ, Yeni P, Danon F, Baumann N (1985). Direct sensitive immunocharacterization of gangliosides on plastic thin-layer plates using Peroxydase staining. J Immun Meth. 78: 135–141.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 1987 Springer-Verlag Berlin Heidelberg

About this paper

Cite this paper

Jauberteau, MO., Richardson, P., Harpin, M.L., Baumann, N. (1987). Determination of Chol-1 Antigen in Human Brain and Neuroblastoma Tumor. In: Rahmann, H. (eds) Gangliosides and Modulation of Neuronal Functions. NATO ASI Series, vol 7. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-71932-5_41

Download citation

  • DOI: https://doi.org/10.1007/978-3-642-71932-5_41

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-71934-9

  • Online ISBN: 978-3-642-71932-5

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics