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Gangliosides and Modulation of Neuronal Functions pp 49–70Cite as

Fundamentals of Structural Analysis of Glycosphingolipids by Proton Nuclear Magnetic Resonance Spectroscopy

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Part of the book series: NATO ASI Series ((ASIH,volume 7))

Abstract

To determine the primary structure of the carbohydrate portion of a glycosphingolipid, it is necessary to establish the composition and configuration of its sugar residues, and the sequence and linkage sites of the oligosaccharide chain. This information has traditionally been obtained by application of a combination of such procedures as compositional analysis by gas-liquid chromatography, mass spectrometry, permethylation studies, Smith degradation, partial acid or enzyme hydrolysis, optical rotation measurements, etc. However, these techniques are time-consuming, require elaborate derivatization of the substance, and access to many different instruments. Furthermore, these procedures do not afford secondary structural information (solution conformation) which is important in understanding the biological properties of these compounds. Proton nuclear magnetic resonance (NMR) spectroscopy, on the other hand, is well-suited for providing the primary and secondary structure of glycosphingolipids because this method is rapid, quantitative, sensitive and nondestructive. However, previous utilization of proton NMR has yielded only incomplete data concerning the primary structure of these substances.

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Authors and Affiliations

  1. Department of Neurology, Yale University School of Medicine, New Haven, Connecticute, USA

    Robert K. Yu

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  1. Robert K. Yu
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Editors and Affiliations

  1. Zoological Institute, University of Stuttgart-Hohenheim, Garbenstr. 30, 7000, Stuttgart 70, Germany

    Hinrich Rahmann

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© 1987 Springer-Verlag Berlin Heidelberg

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Yu, R.K. (1987). Fundamentals of Structural Analysis of Glycosphingolipids by Proton Nuclear Magnetic Resonance Spectroscopy. In: Rahmann, H. (eds) Gangliosides and Modulation of Neuronal Functions. NATO ASI Series, vol 7. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-71932-5_4

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  • DOI: https://doi.org/10.1007/978-3-642-71932-5_4

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-71934-9

  • Online ISBN: 978-3-642-71932-5

  • eBook Packages: Springer Book Archive

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