New Aspects in the Determination of Endogenous Membrane-Bound Sialidase in the CNS, Using 4-MU-NeuAc as an (Exogenous) Artificial Substrate

  • H. Swoboda
  • V. Seybold
  • W. Probst
  • H. Rahmann
Conference paper
Part of the NATO ASI Series book series (volume 7)

Abstract

Determination of endogenous membrane-bound sialidase by means of a fluorometric assay using 4-Methyl-Umbelliferyl-NeuAc (4-MU-NeuAc) as an artificial (exogenous) substrate is commonly used because of its easy applicability, though the opinions regarding the reliability of this assay are partly contrary (Scheel et al.; 1985; Eur. J. Biochem. 153: 29–35; Potier; 1981; Perspectives in inherited metabolic diseases 4: 233–247; Edi. Ermes Mailand). In order to clarify this, an effort has been made to find out the analytical “weak-points” of this test and to apply it to different biological systems (acclimation/acclimatization of dwarf- and golden hamsters; ontogeny of trout and a cichlid fish).

Keywords

Tate Paration 

Copyright information

© Springer-Verlag Berlin Heidelberg 1987

Authors and Affiliations

  • H. Swoboda
    • 1
  • V. Seybold
    • 1
  • W. Probst
    • 1
  • H. Rahmann
    • 1
  1. 1.Institute of ZoologyUniversity of Stuttgart-HohenheimStuttgart 70Germany

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