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New Aspects in the Determination of Endogenous Membrane-Bound Sialidase in the CNS, Using 4-MU-NeuAc as an (Exogenous) Artificial Substrate

  • H. Swoboda
  • V. Seybold
  • W. Probst
  • H. Rahmann
Conference paper
Part of the NATO ASI Series book series (volume 7)

Abstract

Determination of endogenous membrane-bound sialidase by means of a fluorometric assay using 4-Methyl-Umbelliferyl-NeuAc (4-MU-NeuAc) as an artificial (exogenous) substrate is commonly used because of its easy applicability, though the opinions regarding the reliability of this assay are partly contrary (Scheel et al.; 1985; Eur. J. Biochem. 153: 29–35; Potier; 1981; Perspectives in inherited metabolic diseases 4: 233–247; Edi. Ermes Mailand). In order to clarify this, an effort has been made to find out the analytical “weak-points” of this test and to apply it to different biological systems (acclimation/acclimatization of dwarf- and golden hamsters; ontogeny of trout and a cichlid fish).

Keywords

Enzyme Preparation Sodium Salt Artificial Substrate Golden Hamster Biological Result 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Copyright information

© Springer-Verlag Berlin Heidelberg 1987

Authors and Affiliations

  • H. Swoboda
    • 1
  • V. Seybold
    • 1
  • W. Probst
    • 1
  • H. Rahmann
    • 1
  1. 1.Institute of ZoologyUniversity of Stuttgart-HohenheimStuttgart 70Germany

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