In Vitro Incorporation and Metabolism of Gangliosides

  • G. Schwarzmann
  • D. Marsh
  • V. Herzog
  • K. Sandhoff
Part of the NATO ASI Series book series (volume 7)


Gangliosides are ubiquitous in vertebrate tissue and are highly abundant in neuronal plasma membranes (for review see 1–3). The lipophilic ceramide moiety of gangliosides is anchored in the outer leaflet of the lipid bilayer and the hydrophilic sialooligosaccharide residue faces the extracellular space.


Electron Spin Resonance Spectrum Gold Particle Golgi Complex Endocytic Vesicle Golgi Compartment 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Wiegandt H (1982) The gang1iosides. Advances in Neurochemistry 4: 149–223CrossRefGoogle Scholar
  2. 2.
    Svennerholm L (1984) Biological significance of gangliosides. In: Cellular and pathological aspects of glycoconjugate metabolism (Eds. Dreyfus H, Massarelli R, Freysz L, Rebel G) INSERM 126: 21–44Google Scholar
  3. 3.
    Ledeen R (1985) Gangliosides of the neuron. Trends Neurosci. 8: 169–174CrossRefGoogle Scholar
  4. 4.
    Klenk E (1942) Über die Ganglioside, eine neue Gruppe von zuckerhaltigen Gehirnlipoiden. Hoppe-Seyler’s Z. Physiol. Chem. 273: 76–86Google Scholar
  5. 5.
    Hakomori S-I (1981) Glycosphingolipids in cellular interaction, differentiation, and oncogenesis. Annu. Rev. Biochem. 50: 733–764PubMedCrossRefGoogle Scholar
  6. 6.
    Yusuf HKM, Pohlentz G, Schwarzmann G, and Sandhoff K (1983) Ganglioside biosynthesis in Golgi apparatus of rat liver- stimulation by phosphatidyl glycerol and inhibition by tunicamycin. Eur. J. Biochem. 134: 47–54PubMedCrossRefGoogle Scholar
  7. 7.
    Scheel G, Schwarzmann G, Hoffmann-Bleihauer P, and Sandhoff K (1985) The influence of ganglioside insertion into brain membranes on the rate of ganglioside degradation by membrane-bound sialidase. Eur. J. Biochem. 153: 29–35PubMedCrossRefGoogle Scholar
  8. 8.
    Sandhoff K (1984) Function and relevance of activator proteins for glycolipid degradation. In: The molecular basis of lysosomal storage disorders (Eds. Barranger JA, and Brady RO) Academic Press, New York, 19–49Google Scholar
  9. 9.
    Schwarzmann G, Sonderfeld S, Conzelmann E, Marsh D, Sandhoff K (1984) Insertion into cultured cells and metabolism and intracellular transport of exogenous gangliosides. In: Cellular and pathological aspects of glycoconjugate metabolism (Eds. Dreyfus H, Massarelli R, Freysz L, Rebel G) INSERM 126: 195–210Google Scholar
  10. 10).
    Schwarzmann G, Hinrichs U, Sonderfeld S, Marsh D, and Sandhoff K (1986) Metabolism of exogenous gangliosides in cultured fibroblasts and cerebellar cells. In: Enzymes of lipid metabolism II (Eds. Freysz L, Dreyfus H, Massarelli R, Gatt S) Plenum Press, New York, in pressGoogle Scholar
  11. 11).
    Callies R, Schwarzmann G, Radsak K, Siegert R, and Wiegandt H (1977) Characterization of the cellular binding of exogenous gangliosides. Eur. J. Biochem. 80: 425–432PubMedCrossRefGoogle Scholar
  12. 12).
    Radsak K, Schwarzmann G, and Wiegandt H (1982) Studies on the cell association of exogenously added sialoglycolipids. Hoppe-Seyler’s Z. Physiol. Chem. 363: 263–272PubMedCrossRefGoogle Scholar
  13. 13).
    Schwarzmann G., Schubert J, Hoffmann-Bleihauer P, Marsh D, and Sandhoff K (1981) Synthesis of spin-labeled ganglioside analogs and their uptake by cell membranes. In: Glycoconjugates-Proceedings of the sixth International Symposium on Glycoconjugates (Eds. Yamakawa T, Osawa T, and Handa S) Scientific Society Press, Tokyo, 333–334Google Scholar
  14. 14).
    Schwarzmann G, Hoffmann-Bleihauer P, Schubert J, Sandhoff K, and Marsh D (1983) Incorporation of ganglioside analogues into fibroblast cell membranes. A spin-label study. Biochemistry 22: 5041–5048PubMedCrossRefGoogle Scholar
  15. 15).
    Schwarzmann G and Sandhoff K (1987) Lysogangliosides: Synthesis and use in preparing labeled gangliosides. Methods in Enzymolgy 138: 319–341CrossRefGoogle Scholar
  16. 16).
    Sonderfeld S, Conzelmann E, Schwarzmann G, Burg J, Hinrichs U, and Sandhoff K (1985) Incorporation and metabolism of ganglioside GM2 in skin fibroblasts from normal and GM2 gangliosidosis subjects. Eur. J. Biochem. 149: 247–255PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1987

Authors and Affiliations

  • G. Schwarzmann
    • 1
  • D. Marsh
    • 2
  • V. Herzog
    • 3
  • K. Sandhoff
    • 1
  1. 1.Institut für Organische Chemie und Biochemie der Universität BonnBonn 1Germany
  2. 2.Max-Planck-Institut für biophysikalische ChemieGöttingenGermany
  3. 3.Institut für Zellbiologie der Universität MünchenMünchen 2Germany

Personalised recommendations