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Cloning of Murine B-Lymphocyte Differentiation Stage-Specific Genes

  • Conference paper
Differentiation of B Lymphocytes

Part of the book series: Current Topics in Microbiology and Immunology ((CT MICROBIOLOGY,volume 135))

Abstract

During differentiation of eukaryotic cells with specialized functions selected genes must be expressed which encode structures for these functions. We expect that the expression of these selected genes will occur in the differentiated cell type only, and in no other cell of the body, if the corresponding structures and functions are unique for the type of differentiated cell. Examples of selected gene expression are known in blood cells. Globin is expressed in red blood cells, the T-cell receptor for antigen in T cells, and immunoglobulin (Ig) in B cells only.

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References

  • Ahmed A, Scher I, Sharrow SO, Smith AH, Paul WE, Sachs DH, Sell KW (1977) B-lymphocyte heterogeneity: development and characterization of an alio antiserum which distinguishes B-lymphoeyte differentiation alloantigens. J Exp Med 145: 101–110

    Article  PubMed  CAS  Google Scholar 

  • Alt FW, Yancopoulos GD, Blackwell TK, Wood C, Thomas E, Boss M, Coffman R, Rosenberg N, Tonegawa S, Baltimore D (1984) Ordered rearrangement of immunoglobulin heavy chain variable region segments. EMBO J 3: 1209–1219

    PubMed  CAS  Google Scholar 

  • Andersson J, Sjöberg O, Moller G (1972) Induction of immunoglobulin and antibody synthesis in vitro by lipopolysaccharides. Eur J Immunol 2: 349–353

    Article  PubMed  CAS  Google Scholar 

  • Ben-Neriah Y, Bernards A, Paskind M, Daley GQ, Baltimore D (1986) Alternative 5′ exons in c-abl mRNA. Cell 44: 577–586

    Article  PubMed  CAS  Google Scholar 

  • Berger CN (1986a) In situ hybridization of immunoglobulin specific RNA in single cells of the B lymphocyte lineage with radiolabeled DNA probes. EMBO J 5:85–93

    PubMed  CAS  Google Scholar 

  • Berger CN (1986b) In situ Hybridisierung von mRNA Molekülen in einzelnen lymphoiden Zellen und Anwendungen zur Untersuchung der Repertoires Antikörper produzierender Lymphozyten. Dissertation, University of Basle

    Google Scholar 

  • Bruce J, Symington FW, McKearn TJ, Sprent J (1981) A monoclonal antibody discriminating between subsets of T and B cells. J Immunol 127: 2496–2501

    PubMed  CAS  Google Scholar 

  • Chien Y, Becker DM, Lindsten T, Okamura M, Cohen DI, Davis MM (1984) A third type of murine T-receptor gene. Nature 312: 31–35

    Article  PubMed  CAS  Google Scholar 

  • Coffman RL, Weissman IL (1981b) B220: a B cell-specific member of the T200 glycoprotein family. Nature 289: 681–683

    Article  PubMed  CAS  Google Scholar 

  • Cohen DI, Steinberg AD, Paul WE, Davis MM (1985) Expression of an X-linked gene family ( XLR) in late-stage B cells and its alteration by the xid mutation. Nature 314: 372–373

    Article  PubMed  CAS  Google Scholar 

  • Cone RE, Huber B, Cantor H, Gershon RK (1978) Molecular identification of a surface structure on B cells (Lyb-3) and its relationship to B cell triggering. J Immunol 120: 1733–1740

    PubMed  CAS  Google Scholar 

  • Cooper MD, Mulvaney D, Coutinho A, Canave P-A (1986) Identification of a novel cell surface molecular on early B-lineage cells. Nature, to be published

    Google Scholar 

  • Corbel C, Melchers F (1984) The synergism of accessory cells and of soluble α-factors derived from them in the activation of B cells to proliferation. Immunol. Rev. 78: 51–74

    Article  PubMed  CAS  Google Scholar 

  • Coutinho A, Moller G (1975) Thymus-independent B-cell induction and paralysis. Adv Immunol 21: 113–236

    Article  PubMed  CAS  Google Scholar 

  • De Franco AL, Ashwell JD, Schwartz RH, Paul WE (1982) Frequency of B lymphocytes responsive to anti-immunoglobulin. J Exp Med 155: 1523–1536

    Article  Google Scholar 

  • Driel IRV (1985) Murine plasma cell membrane antigen PC-1: Molecular cloning of cDNA and analysis of expression. Proc Natl Acad Sci USA 82: 8619–8623

    Article  PubMed  Google Scholar 

  • Forsgren S, Pobor G, Coutinho A, Pierres M (1984) The role of I-A/E molecules in B lymphocyte activation. I. Inhibition of lipopolysaccharide-induced responses by monoclonal antibodies. J Immunol 133: 2104–2110

    PubMed  CAS  Google Scholar 

  • Hardy RR, Hayakawa K, Parks DR, Herzenberg LA, Herzenberg LA (1984) Murine B cell differentiation lineages. J Exp Med 159: 1169–1188

    Article  PubMed  CAS  Google Scholar 

  • Hayakawa K, Hardy RR, Herzenberg LA, Herzenberg LA (1985) Progenitors for Ly-1 B cells are distinct from progenitors for other B cells. J Exp Med 161: 1554–1568

    Article  PubMed  CAS  Google Scholar 

  • Hedrick SM, Nielsen EA, Kavaler J, Cohen DI, Davis MM (1984b) Sequence relationships between putative T-cell receptor polypeptides and immunoglobulins. Nature 308: 153–158

    Article  PubMed  CAS  Google Scholar 

  • Hieter PA, Stanely J, Korsmeyer, Waldmann TA, Leder P (1981) Human immunoglobulin к light chain genes are deleted or rearranged in λ-producing B cells. Nature 290: 368–372

    Article  PubMed  CAS  Google Scholar 

  • Huber BT, Gershon KK, Cantor H (1977) Identification of a B-cell surface structure involved in antigen-dependent triggering: absence of this structure on B cells from CBA/N mutant mice. J Exp Med 145: 10–16

    Article  PubMed  CAS  Google Scholar 

  • Katz DH, Hamaoka T, Dorf ME, Benacerraf B (1973) Cell interaction between histocompatible T and B lymphocytes. III. Demonstration that the H-2 gene complex determines successful physiologic lymphocyte interactions. Proc Natl Acad Sci USA 70: 2624–2628

    Article  PubMed  CAS  Google Scholar 

  • Kincade PW, Lawton AR, Boekman DE, Cooper MD (1970) Suppression of immunoglobulin G synthesis as a result of antibody–mediated suppression of immunoglobulin M synthesis in chickens. Proc Natl Acad Sci USA 67: 1918–1925

    Article  PubMed  CAS  Google Scholar 

  • Kincade PW, Lee G, Paige CJ, Scheid MP (1981) Antigens displayed on murine B lymphocyte precursors. J Immunol 127: 2262–2266

    PubMed  CAS  Google Scholar 

  • Kishimoto T, Ishizaka K (1975) Regulation of antibody response in vitro. IX. Induction of secondary anti-hapten IgG antibody response by anti-immunoglobulin and enhancing soluble factor. J Immunol 114: 585–591

    PubMed  CAS  Google Scholar 

  • Lambris JD, Dobson NJ, Ross GD (1981) Isolation of lymphocyte membrane complement receptor type two (the C3d receptor) and preparation of receptor-specific antibody. Proc Natl Acad Sci USA 78: 1828–1832

    Article  PubMed  CAS  Google Scholar 

  • Landau NR, St John TP, Weissman IL, Wolf SC, Silverstone AE, Baltimore D (1984) Cloning of terminal transferase cDNA by antibody screening. Proc Natl Acad Sci USA 81: 5836–5840

    Article  PubMed  CAS  Google Scholar 

  • Ledbetter JA, Herzenberg LA (1979) Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens. Immunol Rev 47: 362–384

    Article  Google Scholar 

  • Lemke H, Hämmerling GJ, Hämmerling U (1981) Fine specificity analysis with monoclonal antibodies of antigens controlled by the major histocompatibility complex and by the Q2/TL region in mice. Immunol Rev 47: 175–206

    Article  Google Scholar 

  • Marcu KB, Harris LJ, Stanton LW, Erikson J, Watt R, Croce CM (1983) Transcriptionally active c–myc oncogene is contained within NIARD, a DNA sequence associated with chromosome translocations in B cell neoplasia. Proc Natl Acad Sci USA 80: 519–523

    Article  PubMed  CAS  Google Scholar 

  • McKearn JP, Rosenberg N (1985) Mapping cell surface antigens on mouse pre-B cell lines. Eur J Immunol 15: 295–298

    Article  PubMed  CAS  Google Scholar 

  • McKearn JP, Baum C, Davie JM (1984) Cell surface antigens expressed by subsets of pre-B cells and B cells. J Immunol 132: 332–339

    PubMed  CAS  Google Scholar 

  • Melchers F, Andersson J (1984) B cell activation: three steps and their variations. Cell 37: 715–730

    Article  CAS  Google Scholar 

  • Melchers F, Melchers J, Philips RA (1976) Ontogeny of murine B lymphocytes: development of Ig synthesis and reactivities to mitogens and anti-Ig antibodies. In Cold Spring Harbor Symp Quant Biol 41: 147–158

    Google Scholar 

  • Melchers F, Erdei A, Schulz T (1985) Growth control of activated, synchronized murine B cells by the C3d fragment of human complement. Nature 317: 264–267

    Article  PubMed  CAS  Google Scholar 

  • Möller G (1961) Demonstration of mouse iso-antigens at the cellular level by the fluorescent antibody technique. J Exp Med 114: 415–434

    Article  PubMed  Google Scholar 

  • Mountz JD, Steinberg AD, Klinman DM, Smith HR, Mushinski JF (1984) Autoimmunity and increased c–myb transcription. Science 226: 1087–1089

    Article  PubMed  CAS  Google Scholar 

  • Osmond DG (1979) Generation of B lymphocytes in the bone marrow. In: Cooper M, et al. (eds) B lymphocyte in the immune response. Elsevier North Holland, Amsterdam, pp 63–70

    Google Scholar 

  • Paige CJ, Kincade PW, Ralph P (1978) Murine B cell leukemia line with inducible surface immunoglobulin expression. J Immunol 121: 641–647

    PubMed  CAS  Google Scholar 

  • Parker DC, Frothergill JJ, Wadsworth DC (1979) B lymphocyte activation by insoluble anti-immunoglobulin: induction of immunoglobulin secretion by a T cell-dependent soluble factor. J Immunol 123: 931–941

    PubMed  CAS  Google Scholar 

  • Parkhouse M (1985) in Mechanisms of B cell neoplasia 1985. Workshop at the Basel Institute for Immunology, pp 48–54, Editiones Roche, Basel

    Google Scholar 

  • Pernis B, Chiappino G, Kelus AS, Gell PGH (1965) Cellular localization of immunoglobulins with different allotypic specificities in rabbit lymphoid tissues. J Exp Med 122: 853–876

    Article  PubMed  CAS  Google Scholar 

  • Ross GD, Medof ME (1985) Membrane complement receptors specific for bound fragments of C3. Adv Immunol 37: 217–267

    Article  PubMed  CAS  Google Scholar 

  • Sakaguchi N, Kishimoto T, Kikutani H, Watanabe T, Yoshida N, Shimizu A, Yamawaki-Kataoka Y, Honjo T, Yamamura Y (1980) Induction and regulation of immunoglobulin expression in a murine pre-B cell line, 70Z/3. I. Cell cycle associated induction of sIgM expression and к-chain synthesis in 70Z/3 cells by LPS stimulation. J Immunol 125: 2654–2659

    PubMed  CAS  Google Scholar 

  • Sakaguchi N, Berger CN, Melchers F (1986) Isolation of a cDNA copy of an RNA species expressed in murine pre-B cells. EMBO J 5: 2139–2147

    PubMed  CAS  Google Scholar 

  • Saito H, Kranz DM, Takagaki Y, Hayday AC, Eisen HN and Tonegawa S (1984a) Complete primary structure of a heterodimeric T-cell receptor deduced from cDNA sequences. Nature 309: 757–762

    Article  PubMed  CAS  Google Scholar 

  • Sato H, Boyse EA (1976) A new alloantigen expressed selectively on B cells: the Lyb-2 system. Immunogenetics 3: 565–572

    Article  Google Scholar 

  • Shen FW, Yakura H, Tung J-S (1981) Some compartments of B cell differentiation. Immunol Rev 69: 69–80

    Article  Google Scholar 

  • Shen FW, Saga Y, Litman G, Freeman G, Tung J-S, Cantor H, Boyse EA (1985) Cloning of Ly-5 cDNA. Proc Natl Acad Sci USA 82: 7360–7363

    Article  PubMed  CAS  Google Scholar 

  • Sitia R, Abbott J, Hammerling U (1979) The ontogeny of B lymphocytes. V. Lipopolysaccharide-induced changes of IgD expression on murine B lymphocytes. Eur J Immunol 9: 859–864

    Article  PubMed  CAS  Google Scholar 

  • Sprent J (1978) Role of H–2 gene products in the function of T helper cells from normal and chimeric mice measured in vivo. Immunol Rev 42: 108–137

    Article  PubMed  CAS  Google Scholar 

  • Stearne PA, van Driel IR, Greco B, Simpson RJ, Goding JW (1985) The murine plasma cell antigen PC-1: Purification and partial amino acid sequence. J Immunol 134: 443–448

    PubMed  CAS  Google Scholar 

  • Subbarao B, Mosier DE (1983) Lyb antigens and their role in B lymphocyte activation. Immunol Rev 69: 81–98

    Article  CAS  Google Scholar 

  • Sugiyama H, Akira S, Kikutani H, Kishimoto S, Yamamura Y, Kishimoto T (1983). Functional V region formation during in vitro culture of a murine immature B precursor cell line. Nature 303: 812–815

    Article  PubMed  CAS  Google Scholar 

  • Tada N, Kimura S, Hoffmann M, Hämmerling U (1980) A new surface antigen (PC.2) expressed exclusively on plasma cells. Immunogenetics 11: 351–359

    Article  PubMed  CAS  Google Scholar 

  • Takahashi T, Old, LJ, Boyse EA (1970) Surface alloantigens of plasma cells. J Exp Med 131: 1325–1332

    Article  PubMed  CAS  Google Scholar 

  • Thomas ML, Barclay AN, Gragnon J, Williams AF (1985) Evidence from cDNA clones that the rat leukocyte-common antigen (T200) spans the lipid bilayer and contains a cytoplasmic domain of 80000 Mr. Cell 41: 83–93

    Article  PubMed  CAS  Google Scholar 

  • Tidmarsh GT, Dailey MO, Whitlock CA, Pillemer E, Weissman IL (1985) Transformed lymphocytes from Abelson-diseased mice express levels of a B lineage transformation-associated antigen elevated from that found on normal lymphocytes. J Exp Med 162: 1421–1434

    Article  PubMed  CAS  Google Scholar 

  • Tonegawa S (1983) Somatic generation of antibody diversity. Nature 302: 575–581

    Article  PubMed  CAS  Google Scholar 

  • Tsujimoto Y, Jaffe E, Cossman J, Gorham J, Nowell PC, Croce CM (1985a) Clustering of break-points on chromosome 11 in human B-cell neoplasms with the t(l1:14) chromosome translocation. Nature 315:340–343

    Article  PubMed  CAS  Google Scholar 

  • Tsujimoto Y, Cossman J, Jaffe E, Croce CM (1985b) Involvement of the bcl-2 gene in human follicular lymphoma. Science 228:1440–1443

    Article  PubMed  CAS  Google Scholar 

  • Unkeless JC, Howard F, Mellman IS (1981) Structural aspects and heterogeneity of immunoglobulin Fc receptors. Adv Immunol 31:247–270

    Article  PubMed  CAS  Google Scholar 

  • Velardi A, Cooper MD (1984) An immunofluorescence analysis of the ontogeny of myeloid, T, and B lineage cells in mouse hemopoietic tissues. J Immunol 133: 672–677

    PubMed  CAS  Google Scholar 

  • Warner NL (1974) Membrane immunoglobulins and antigen receptor on B and T lymphocytes. Adv Immunol 19: 67–216

    Article  PubMed  CAS  Google Scholar 

  • Warner NL, Daley MJ, Richey J, Spellman C (1979) Flow cytometry analysis of murine B lymphoma differentiation. Immunol Rev 48: 197–216

    Article  PubMed  CAS  Google Scholar 

  • Wetzel GD, Kettman JR (1981) Activation of murine B lymphocytes. III. Stimulation of B lymphocyte clonal growth with lipopolysaccharide and dextran sulfate. J Immunol 126: 723–728

    PubMed  CAS  Google Scholar 

  • Yancopoulos GD, Desiderio SV, Paskind M, Kearney JF, Baltimore D, Alt FW (1984) Preferential utilization of the most JH-proximal VH gene segments in pre-B cells lines. Nature 290: 368–372

    Google Scholar 

  • Young RA, Davis RW (1983) Efficient isolation of genes by using antibody probes. Proc Natl Acad Sci USA 84: 1194–1198

    Article  Google Scholar 

  • Zimmerman KA, Yancopoulos GD, Collum RG, Smith RK, Kohl NE, Denis KA, Nau MM, et al. (1986) Differential expression of myc family genes during murine development. Nature 314: 780–783

    Article  Google Scholar 

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Authors and Affiliations

  1. Basel Institute for Immunology, Grenzacherstrasse 487, 4058, Basel, Switzerland

    N. Sakaguchi, C. N. Berger & F. Melchers

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  1. N. Sakaguchi
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  2. C. N. Berger
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  3. F. Melchers
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Editors and Affiliations

  1. Basel Institute for Immunology, Grenzacherstrasse 487, CH-4058, Basel, Switzerland

    Christopher J. Paige  & Roland H. Gisler  & 

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© 1987 Springer-Verlag Berlin Heidelberg

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Sakaguchi, N., Berger, C.N., Melchers, F. (1987). Cloning of Murine B-Lymphocyte Differentiation Stage-Specific Genes. In: Paige, C.J., Gisler, R.H. (eds) Differentiation of B Lymphocytes. Current Topics in Microbiology and Immunology, vol 135. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-71851-9_10

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  • DOI: https://doi.org/10.1007/978-3-642-71851-9_10

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-71853-3

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