Abstract
Hyaluronic acid (HA) detection is particularly important during vertebrate embryonic development since it is believed to play a significant role in the differentiation and migration processes which occur in mesenchyme (Toole, 1973; Pratt et al., 1975; Toole, 1981) and in nervous tissue (Brunngraber, 1979). We report here results about HA detection during the development of rat cerebral cortex. HA location was compared to that of hyaluronectin (HN) which is characterized in vitro by its affinity for HA (Delpech and Halavent, 1981; Delpech, 1982). Recent results led to the conclusion that HN is the protein core of a small human brain proteoglycan (Delpech et al., 1986). Tissue HN detection was made by means of indirect immunohistochemical techniques using anti-HN antibodies. Tissue HA detection was made with the immune complex technique, using HN as a probe for HA. We describe in this paper the details of techniques, the preparation of reagents and the specificity criteria selected. In order to define precise topographic landmarks in the developing cerebral cortex, we refer to previous work on brain ontogenesis (Boulder Committee, 1970; Marin Padilla, 1978; Readier et al., 1980; Pinto-Lord and Caviness, 1979).
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References
Bertrand, P., and B. Delpech (1985) Interaction of hyaluronectin with hyaluronic acid oligosaccharides. J. Neurochem. 45: 434–439.
Boulder Committee (1970) Embryonic vertebrate central nervous system, revised termi-nology. Anat. Record 166: 257–261.
Brückner, G., and D. Biesold (1981) Wheat germ agglutinin indicates the commencement of neuronal differentiation in the embryonic brain. A comparative carbohydrate histochemical study. Acta histochem. 69: 231–242.
Brückner, G., L. Muller, L. Wollweber, R. Samtleben, and D. Biesold (1985) Lectin binding sites and anionic components related to differentiation in the prenatal rat cerebral cortex. J. Hirnforsch. 26: 615–634.
Brunngraber, E.G. (1979). In Neurochemistry of Aminosugars, E.G. Brunngraber, ed., pp. 128–177. C.C. Thomas Publ., Springfield, IL.
Delpech, B., and C. Halavent (1981) Characterization and purification from human brain of a hyaluronic acid-binding glycoprotein: Hyaluronectin. J. Neurochem. 38: 978–984.
Delpech, A., N. Girard, and B. Delpech (1982) Localization of hyaluronectin in the nerv-ous system. Brain Res. 245: 251–257.
Delpech, A., and B. Delpech (1984) Expression of hyaluronic acid-binding glycoprotein, hyaluronectin, in the developing rat embryo. Develop. Biol. 101: 391–400.
Delpech, B., P. Bertrand, B. Hermelin, A. Delpech, N. Girard, E. Halkin, and C. Chauzy (1986) Hyaluronectin. In Frontiers of Matrix Biology, L. Robert, ed. Karger Pub-lish., Basel, Switzerland, in press.
Derer, P. (1985) Comparative localization of Cajal-Retzius cells in the neocortex of normal and reeler mutant mice fetuses. Neurose. Letters 54: 1–6.
Derer, P., and S. Nakanishi (1983) Extracellular matrix distribution during neocortical wall ontogenesis in “normal” and “reeler” mice. J. Hirnforsch. 24: 209–224.
Girard, N., P. Bertrand, A. Delpech, and B. Delpech (1984) Caracterisation tissulaire de l’acide hyaluronique dans le cervelet par la hyaluronectine. Comptes Rendus Acad. Sc. (Paris) serie III, 298: 325–330.
Girard, N., A. Delpech, and B. Delpech (1986) Characterization of hyaluronic acid on tissue sections with hyaluronectin. J. Histochem. Cytochem. 34: 539–541.
Hay, E.D., and S. Meier (1974) Glycosaminoglycan synthesis by embryonic inductors: Neural tube, notochord and lens. J. Cell Biol. 62: 889–898.
Knudson, C.B., and B.P. Toole (1985) Fluorescent morphological probe for hyaluronate. J. Cell Biol. 100: 1753–1758.
König, N., G. Roch, and R. Marty (1975) The onset of synaptogenesis in the rat temporal cortex. Anat Embryol. 148: 73–87.
Marin-Padilla, M. (1978) Dual origin of the mammalian neocortex and evolution of the cortical plate. Anat. Embryol. 152: 109–126.
Morris, G.M., and M. Solursh (1978) Regional differences in mesenchymal cell morphology and glycosaminoglycans in early neural-fold stage embryos. J. Embryol. exp. morphol. 46: 37–52.
Pinto-Lord, M., and V.S. Caviness, Jr. (1979) Determinants of cell shape and orientation: A comparative Golgi analysis of cell-axon interrelationships in the developing neocortex of normal and reeler mice. J. Comp. Neurol. 187: 49–70.
Pratt, R.M., M.A. Larsen, and M.C. Johnston (1975) Migration of cranial neural crest cells in a cell-free hyaluronate-rich matrix. Develop. Biol. 298–305.
Readier, E., A. Readier, and S. Feldhaus (1980) Dynamic aspects of neocortical histo¬genesis in the rat. Anat. Embryol. 158: 253–269.
Ripellino, J.A., M.M. Klinger, R.U. Margolis, and R.K. Margolis (1985) The hyaluronic acid binding region as a specific probe for the localization of hyaluronic acid in tissue sections. Application to chick embryo and rat brain. J. Histochem. Cytochem. 33: 1060–1066.
Toole, B.P. (1973) Hyaluronate and hyaluronidase in morphogenesis and differentiation. Amer. Zool. 13: 1061–1065.
Toole, B.P. (1981) Glycosaminoglycans in morphogenesis. In Cell Biology of Extracellular Matrix, E.D. Hay, ed., pp. 259–294. Plenum, New York.
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Delpech, A., Delpech, B., Girard, N., Bertrand, P., Chauzy, C. (1987). Hyaluronectin and Hyaluronic Acid During the Development of Rat Brain Cortex. In: Wolff, J.R., Sievers, J., Berry, M. (eds) Mesenchymal-Epithelial Interactions in Neural Development. NATO ASI Series, vol 5. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-71837-3_6
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DOI: https://doi.org/10.1007/978-3-642-71837-3_6
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