Role of Medium and Substratum on Proliferation of Astroglia in Vitro

  • A. Michler-Stuke
Conference paper
Part of the NATO ASI Series book series (volume 5)

Abstract

In the adult brain astrocytes represent a multifunctional cell population. Uptake and/or release of putative neurotransmitters like GABA, glutamate and taurine (Schousboe et al., 1976; Sellstrom and Hamberger, 1977; Balcar et al., 1977) are among the various functions in which they are involved. By taking up substances like potassium (Somjen, 1975) they may also play a role in the regulation of the extracellular ionic environment in the nervous system.

Keywords

Hydrocortisone Heparin Trypsin Stein Neuroblastoma 

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References

  1. Albrecht, J., B. Wroblewska, and M.J. Mossakowski (1982) The binding of insulin to cerebral capillaries and astrocytes of the rat. Neurochem. Res. 7: 489–494.PubMedCrossRefGoogle Scholar
  2. Aquino, D.A., R.U. Margolis, and R.K. Margolis (1984) Immunocytochemical localization of a chondroitin sulfate proteoglycan in nervous tissue. II. Studies in developing brain. J. Cell. Biol. 99: 1130–1139.PubMedCrossRefGoogle Scholar
  3. Balcar, V.J., J. Borg, and P. Mandel (1977) High affinity uptake of L-glutamate and L-Google Scholar
  4. aspartate by glial cells. J. Neurochem. 28: 87–93.Google Scholar
  5. Carpenter, G., and S. Cohen (1979) Epidermal growth factor. Ann. Rev. Biochem. 48: 193–216.PubMedCrossRefGoogle Scholar
  6. Delpech, A., and B. Delpech (1984) Expression of hyaluronic acid-ginding glycoprotein, hyaluronectin, in the developing rat embryo. Dev. Biol. 101: 391–400.PubMedCrossRefGoogle Scholar
  7. Fischer, G., A. Leutz, and M. Schachner (1982) Cultivation of immature astrocytes of mouse cerebellum in a serum-free, hormonally defined medium. Appearance of the mature astrocytic phenotype after addition of serum. Neurosci. Lett. 29: 279–302.CrossRefGoogle Scholar
  8. Gospodarowicz, D., D. Delgado, and I. Vlodavsky (1980) Permissive effect of the extracellular matrix on cell proliferation. Proc. Natl. Acad. Sci. (USA) 77: 4094–4098.CrossRefGoogle Scholar
  9. Gospodarowicz, D., G. Greenburg, H. Bialecki, and B.R. Zetter (1978) Factors involved in the modulation of cell proliferation in vivo and in vitro: The role of fibroblast and epidermal growth factors in the proliferative response of mammalian cells. In Vitro 14: 85–118.Google Scholar
  10. Grobstein, C. (1975) Developmental role of intracellular matrix: Retrospective and prospective. In Extracellular Matrix Inifluences on Gene Expression, H. Slavkin and R. Greulich, eds., pp. 6–16. Academic Press, New York.Google Scholar
  11. Havrankova, J., J. Roth, and M. Brownstein (1978) Insulin receptors are widely distributed in the central nervous system of the rat. Nature, Lond. 272: 827–829.CrossRefGoogle Scholar
  12. Hay, E.D. (1980) Development of the vertebrate cornea. Int. Rev. Cytol. 633: 263–322.CrossRefGoogle Scholar
  13. Heldin, C., A. Wasteson, and B. Westermark (1980) Growth of normal human glial cells in a defined medium containing platelet-derived growth factor. Proc. Natl. Acad. Sci. (USA) 77: 6611–6615.CrossRefGoogle Scholar
  14. Hynes, R.O. (1981) Relationships between fibronectin and the cytoskeleton. Cell Surface Rev. 7: 97–136.Google Scholar
  15. Lembach, K.J. (1976) Enhanced synthesis and extracellular accumulation of hyaluronic acid during stimulation of quiescent human fibroblasts by mouse epidermal growth factor. J. Cell Physiol. 89: 277–288.PubMedCrossRefGoogle Scholar
  16. Leutz, A., and M. Schachner (1981) Epidermal growth factor stimulates DNA-synthesis of astrocytes in primary cerebellar cultures. Cell Tissue Res. 220: 393–404.PubMedCrossRefGoogle Scholar
  17. Liesi, P., T. Kirkwood, and A. Vaheri (1986) Fibronectin is expressed by astrocytes cultured from embryonic and early postnatal rat brain. Exp. Cell. Res. 163: 175–185.PubMedCrossRefGoogle Scholar
  18. Margolis, R.U., R.K. Maragolis, L.B. Chang, and C. Pret (1975) Glycosaminoglycans of brain during development. Biochemistry 14: 85–88.PubMedCrossRefGoogle Scholar
  19. Michler-Stuke, A., J.R. Wolff, and J. Bottenstein (1984) Factors influencing astrocyte growth and development in defined media. Int. J. Devi. Neuroscience 2: 575–584.CrossRefGoogle Scholar
  20. Michler-Stuke, A., and J. Bottenstein (1982) Proliferation of glial-derived cells in defined media. J. Neurosci. Res. 7: 215–228.PubMedCrossRefGoogle Scholar
  21. Michler-Stuke, A., and J. Bottenstein (1981) Homotypic extracellular matrices enhance the proliferation of human neuroblastoma cells. Soc. Neurosci. Abstr. 1: 149.Google Scholar
  22. Morrisson, R., and J. De Vellis (1981) Growth of purified astrocytes in a chemically defined medium. Proc. Natl. Acad. Sci. (USA) 78: 7205–7209.CrossRefGoogle Scholar
  23. Nave, K.A., R. Probstmeier, and M. Schachner (1985) Epidermal growth factor does not cross the blood-brain barrier. Cell Tissue Res. 241: 453–457.PubMedCrossRefGoogle Scholar
  24. Raizada, M.K., J.F. Stamler, J. Quinlan, S. Landas, and M.I. Phillips (1982) Identification of insulin receptor containing cells in primary cultures of rat brain. Cell. Mol. Neurobiol. 2: 47–52.CrossRefGoogle Scholar
  25. Ripellino, J.A., M.M. Klinger, R.U. Margolis, and R.K. Margolis (1985) The hyaluronic acid binding region as a specific probe for the localization of hyaluronic acid in tissue sections. J. Histochem. Cytochem. 33: 1060–1066.PubMedCrossRefGoogle Scholar
  26. Schousboe, A., H. Fosmark, and G. Svenneby (1976) Taurine uptake in astrocytes cultured from dissociated mouse brain hemispheres. Brain Res. 116: 158–164.PubMedCrossRefGoogle Scholar
  27. Sellstrom, A., and A. Hamberger (1977) Potassium-stimulated-aminobutyric acid release from neurons and glia. Brain Res. 119: 189–198.PubMedCrossRefGoogle Scholar
  28. Simpson, D.L., R. Morrisson, J. De Vellis, and H.R. Herschman (1982) Epidermal growth factor binding and mitogenic activity on purified populations of cells from the central nervous system. J. Neurosci. Res. 8: 453–462.PubMedCrossRefGoogle Scholar
  29. Singh, M., E.V. Chandrasekaran, E. Cherian, and B.K. Bacchawat (1969) Isolation and characterization of glycosaminoglycans in brain and different species. J. Neuro- chem. 16: 1157–1162.Google Scholar
  30. Somjen, G.G. (1975) Electrophysiology of neuroglia. Ann. Rev. Physiol. 37: 163–190.CrossRefGoogle Scholar
  31. Toole, B.P. (1976) Morphogenetic role of glycosaminoglycans (acid mucopolysaccharides) in brain and other tissue. In Neuronal Recognition, S.H. Barondes, ed., pp. 275–329. Chapman and Hall, London.Google Scholar
  32. Weibel, M., B. Pettmann, G. Daune, G. Labourdette, and M. Sensenbrenner (1984) Chemically defined medium for rat astroglial cells in primary culture. Int. J. Devi. Neuroscience 2: 355–366.CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Hedelberg 1987

Authors and Affiliations

  • A. Michler-Stuke
    • 1
  1. 1.Zentrum AnatomieUniversität GöttingenGöttingenFederal Republic of Germany

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