Skip to main content

Part of the book series: Handbook of Experimental Pharmacology ((HEP,volume 82))

Abstract

Immunologic assays are based on the use of two reagents: an antibody and a labeled molecule, whose properties directly limit the quality of the measurement. The antibody constitutes the principal element of the assay and defines the specificity of the method through the particular characteristics of its binding to the antigen.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Abraham GE (1974) Radioimmunoassay of steroids in biological material. Proc symp radioimmunoassay relat proc med, Istambul. IAEA-SM. 177/207 2: 3–29

    Google Scholar 

  • Al-Bassam MN, O’Sullivan MJ, Bridges JW, Marks V (1979) An improved methotrexate enzyme-immunoassay. 25: 1448–1452

    CAS  Google Scholar 

  • Alder FL, Liu C-T (1971) Detection of morphine by hemagglutination-inhibition. J Immunol. 106: 1684–1685

    Google Scholar 

  • Alexander NM (1974) Oxidative cleavage of tryptophanyl peptide bonds during chemical and peroxidase-catalyzed iodination. J Biol Chem. 249: 1946–1952

    PubMed  CAS  Google Scholar 

  • Anderson GW, Zimmerman JE, Callahan FM (1964) The use of esters of N-hydroxysuc-cinimide in peptide synthesis. J Am Chem Soc. 86: 1839–1842

    CAS  Google Scholar 

  • Andrieu JM, Mamas S, Dray F (1975) Viroimmunoassay of steroids: method and principles. In: Campbell EHD, Hiller SG, Griffiths K (eds) Steroid immunoassay. Proceedings of the fifth Tenovus workshop, Cardiff, April 1974. Alpha Omega, Cardiff, pp 189–198

    Google Scholar 

  • Antonov PA, Pancheva RP, Ivanov IG (1985) Radiodination of naturally occurring phos-pholipids. Biochim Biophys Acta. 835: 408–10

    PubMed  CAS  Google Scholar 

  • Arakawa H, Maeda M, Tsuji A (1985) Chemiluminescence enzyme immunoassay for tyrosin with use of glucose oxydase and a bis (2,4,6-Trichlorophenyl) oxalate- fluorescent dye system. Clin Chem. 31: 430–434

    PubMed  CAS  Google Scholar 

  • Argentini M (1982) Labelling with iodine. A review of the literature. Federal Institute for Reactor Research, Wuerenlingen, Switzerland

    Google Scholar 

  • Assoian RK, Blix PM, Rubenstein AH, Tager HS (1980) Iodotyrosylation of peptides using tertiary-butyloxycarbonyl-L-[125I]iodotyrosine N-hydroxysuccinimide ester. Anal Biochem. 103: 70–76

    PubMed  CAS  Google Scholar 

  • Aubert ML (1971) Critical study of the radioimmunological assay for the dosage of the polypeptide hormone in plasma. Minerva Medica, Torino, p 1

    Google Scholar 

  • Avrameas S (1969) Coupling of enzymes to proteins with glutaraldehyde. Immunochemistry. 6: 43–52

    PubMed  CAS  Google Scholar 

  • Avrameas S, Terninck T (1971) Peroxydase labelled antibody and Fab conjugates with enhanced intracellular penetration. Immunochemistry. 8: 1175–1179

    PubMed  CAS  Google Scholar 

  • Avrameas S, Terninck T, Guesdon JL (1978) Coupling of enzymes to antibodies and antigens. Scand J Immunol. 8: 7–23

    Google Scholar 

  • Bacquet C, Twumasi DY (1984) A homogeneous enzyme immunoassay with avidine-li-gand conjugates as the enzyme modulator. Anal Biochem. 136: 487–490

    PubMed  CAS  Google Scholar 

  • Barnard GJ, Collins WP, Kohen F, Linner HR (1981) The measurement of urinary estriol- 16a-glucuronide by a solid phase chemiluminescence immunoassay. J Steroid Biochem. 14: 941–948

    PubMed  CAS  Google Scholar 

  • Bastiani RJ, Phillips RC, Schneider RS, Ullman EF (1973) Homogeneous immunochemi–cal drug assays. Am J Med Technol. 39: 211–216

    PubMed  CAS  Google Scholar 

  • Bayly JR, Evans EA (1968) Storage and stability of compounds labelled with radioisotopes. Review 7. Radiochemical Centre, Amersham, p 1

    Google Scholar 

  • Bayly JR, Evans EA, Glover JS, Rabinowitz JL (1976) Synthesis of labelled compounds. In: Tubis M, Wolf W (eds) Radiopharmacy. Wiley, New York, p 303

    Google Scholar 

  • Berridge M, Jiang VW, Welch MJ (1979) Local effects in labeled molecules following iodine 125 decay. J Nucl Med. 20: 616

    Google Scholar 

  • Berson SA, Yalow RS, Baumann A, Rotschild MA, Newerly K (1956) Insulin 131I metabolism in human subjects: demonstration of insulin binding globulin in the circulation of insulin treated subjects. J Clin Invest. 35: 170–190

    PubMed  CAS  Google Scholar 

  • Bismuth J, Castay M, Lissitzky S (1971) Sephadex filtration of serum iodocompounds and protein binding of iodotyrosines. Clin Chim Acta. 35: 285–298

    PubMed  CAS  Google Scholar 

  • Bloch HS, Ray FE (1946) Organic radioiodo compounds for cancer research. J Natl Cancer Inst. 7: 61–66

    PubMed  CAS  Google Scholar 

  • Bogdanove EM, Strash AM (1975) Radioiodine escape is an unexpected source of radioim-munoassay error and chronic low level environmental contamination. Nature. 257: 426–427

    PubMed  CAS  Google Scholar 

  • Bolton AE (1977) Radioiodination techniques. Review 18. Radiochemical Centre, Amers- ham, England, p 1

    Google Scholar 

  • Bolton AE, Hunter WM (1973) The labelling of proteins to high specific radioactivities by conjugation to a 125I-containing acylating agent. Biochem J. 133: 529–539

    PubMed  CAS  Google Scholar 

  • Boorsma DM, Streefkerk JG (1979) Periodate or glutaraldehyde for preparing peroxidase conjugate. J Immunol Methods. 30: 245–255

    PubMed  CAS  Google Scholar 

  • Brossier P, Moise C (1982) Exemples d’utilisation d’un complexe organométallique comme marqueur de molécules thérapeutiques. In: Radioimmunoassay and related procedures in medicine, Vienna, IAEA SM-259/35:779–786

    Google Scholar 

  • Brossier P, Moise C (1984) Dosage du fer dans le ferocène et quelques dérivés par spectro-métrie d’absorption atomique sans flamme. Analysis 12: 223 - 224

    CAS  Google Scholar 

  • Brown BL, Reith WS (1967) A method of radio-iodination on a submicrogram scale. The preparation and stability of 131I and 125I-labelled 3-monoiodotyrosine and 3,5-diiodo- tyrosine of very high specific activity. Biochim Biophys Acta. 148: 423–134

    PubMed  Google Scholar 

  • Brunings KJ (1947) Preparation and properties of the iodohistidines. J Am Chem Soc. 69: 205–208

    PubMed  CAS  Google Scholar 

  • Burd JF, Wong RC, Feeney JE, Carrico RJ, Bogulasky RC (1977) Homogeneous reactant- labeled fluorescent immunoassay for therapeutic drugs exemplified by gentamicin determination in human serum. Clin Chem. 23: 1402–1408

    PubMed  CAS  Google Scholar 

  • Butler VP Jr (1973) Radioimmunoassay and competitive binding radioassay methods for the measurement of drugs. Metabolism. 22: 1145–1153

    PubMed  CAS  Google Scholar 

  • Butt WR (1972) The iodination of follicle stimulating and other hormones for radioimmunoassay. J Endocrinol. 55: 453–454

    PubMed  CAS  Google Scholar 

  • Cailla HL, Racine-Weisbuch MS, Delaage MA (1973) Adenosine 3′,5′ cyclic monophos¬phate assay at 10–15 mol level. Anal Biochem. 56: 394–407

    PubMed  CAS  Google Scholar 

  • Cais M (1979) Tests immunologiques à l’aide de complexes organométalliques. Unnouveau concept. Actual Chem. 7: 14–26

    Google Scholar 

  • Cais M (1983) Metalloimmunoassay: Principles and practice. Methods Enzymol. 92: 445–458

    PubMed  CAS  Google Scholar 

  • Cais M, Tirosh N (1981) Metalloimmunoassay. IV. Manganese labelled metallohaptens via cymantrene derivatives. Bull Soc Chem Belg. 90: 27–35

    CAS  Google Scholar 

  • Cais M, Dani S, Eden Y, Gandolfi O, Horn M, Isaacs EE, Josephy Y, Saar Y, Slouin E, Snarsky L (1977) Metalloimmunoassay. Nature. 270: 534–535

    PubMed  CAS  Google Scholar 

  • Cais M, Slouin E, Snarsky L (1978) Metalloimmunoassay. II. Iron-metallohaptens from estrogen steroids. J Organomet Chem. 160: 223–230

    CAS  Google Scholar 

  • Cambiaso CL, Leek AE, De Steewinkel F, Billen J, Masson PL (1977) Particle counting immunoassay (PACIA) I. A general method for the determination of antibodies, antigens, and haptens. J Immunol Meth. 18: 33–44

    CAS  Google Scholar 

  • Cardoso MT, Pradelles P (1982) Preparation of N(l-ethyl-2-pyrrolidyl-methyl) 2-methoxy- 4-iodo-125I-5-ethyl sulfonyl benzamide: a radioligand for the radioimmunoassay of sulpiride-related compounds. J Label Comp Radio. 19: 1103–1109

    CAS  Google Scholar 

  • Carlson RA, White RM (1963) Formation of fragment ions from CH3Te125 and C2H5Te125 following the nuclear decays of CH3125I and C2H5125I. J Chem Phys. 38: 2930–2934

    CAS  Google Scholar 

  • Carlsson J, Drevin H, Axen R (1978) Protein thiolation and reversible protein-protein con-jugation. Biochem J. 173: 723–737

    PubMed  CAS  Google Scholar 

  • Caro RA, Ciscato VA, De Giacomini SMV, Quiroga S (1975) Labeling of proteins with 125I and experimental determination of its specific activity. Int J Appl Radiat Isotopes. 26: 527–532

    CAS  Google Scholar 

  • Carrico RJ, Cristner JE, Boguslaski RC, Yeung KK (1976) A method for monitoring specific binding reactions with cofactor labeled ligands. Anal Biochem. 72: 271–282

    PubMed  CAS  Google Scholar 

  • Carrié ML, Térouanne B, Brochu M, Nicolas JC, Crastes de Paulet A (1986) Bioluminescent immunoassay of progesterone: a comparative study of three different procedures. Anal Biochem. 154: 126–131

    Google Scholar 

  • Castro A, Monji N (1981) Steric-hindrance enzyme immunoassay (SHEIA) using ß-galac- tosidase as an enzyme label and maleimide derivative of hapten (or antigen) for enzyme coupling. Methods Enzymol. 73: 523–542

    CAS  Google Scholar 

  • Christian CL, Mendes-Bryan R, Larson DL (1958) Latex agglutination test for disseminated lupus erythematosus. Proc Soc Exp Biol Med. 98: 820–823

    PubMed  CAS  Google Scholar 

  • Clark PMS, Price CP (1986) Enzyme-amplified immunoassay: a new ultrasensitive assay of thyrotropin evaluated. Clin Chem. 32: 88–92

    PubMed  CAS  Google Scholar 

  • Clinical Chemistry (1983) Product guide for radioassay and nonisotopic ligand assays. Clin Chem. 29: 890–986

    Google Scholar 

  • Cohen Y (1976) Purity and stability of labeled compounds. In: Tubis M, Wolf W (eds) Radiopharmacy, Wiley, New York, p 379

    Google Scholar 

  • Collignon F, Pradelles P (1984) Highly sensitive and specific radioimmunoassays for dihy- droergotoxine components in plasma. Eur J Nucl Med. 9: 23–27

    PubMed  CAS  Google Scholar 

  • Commerford SL (1971) Iodination of nucleic acid in vitro. Biochemistry. 10: 1993–1999

    PubMed  CAS  Google Scholar 

  • Coons AH, Creech HJ, Jones RW (1941) Immunological properties of an antibody containing a fluorescent group. Proc Soc Exp Biol Med. 47: 200–202

    CAS  Google Scholar 

  • Corric JET, Hunter WM (1981) 125Iodinated tracers for hapten specific radioimmunoassays. Methods Enzymol. 73: 79

    Google Scholar 

  • Crowl CP, Gibbons I, Schneider RS (1980) Recent advances in homogeneous enzyme immunoassays for haptens and proteins. In: Nakamura RM, Dito WR, Tucken ES III (eds) Immunoassays clinical laboratory techniques for the 1980s. Liss, New York, pp 89–126

    Google Scholar 

  • Cvoric J (1969) Chemical forms of iodine in carrier free preparations of Na125I. J Chroma- togr. 44: 349–361

    CAS  Google Scholar 

  • Dakubu S, Ekins RP, Jackson T, Marshall NJ (1984) High sensitivity, pulsed light time- resolved fluoroimmunoassay. In: Butt WR (ed) Practical immunoassay, the state of art. Dekker, New York, pp 71–101

    Google Scholar 

  • Damle SR, Advani SH, Desai PB (1982) Non-isotopic analytical methods as alternative to radioimmunoassay in developing countries: a comparative study with reference to biological markers. In: Radioimmunoassay and related procedures in medicine. Vienna, IAEA-SM-259/84, pp 735–742

    Google Scholar 

  • Dandliker WB, Kelly RJ, Dandliker J, Farquhar J, Levin J (1973) Fluorescence polarization immunoassay. Theory and experimental method. Immunochemistry. 10: 219–227

    PubMed  CAS  Google Scholar 

  • David GS, Reisfeld RA (1974) Protein iodination with solid state lactoperoxydase. Biochemistry. 13: 1014–1021

    PubMed  CAS  Google Scholar 

  • De Boever J, Kohen F, Vandekerckhove D, van Maele G (1984) Solid-phase chemilumi- nescence immunoassay for progesterone in unextracted serum. Clin Chem. 30: 1637–1641

    PubMed  Google Scholar 

  • De Luca MA (1978) Bioluminescence and chemiluminescence. Methods Enzymol 57:1 Dewanjee MK, Rao SA (1983) Principles of radioiodination and iodine-labeled tracers in biochemical investigation. In: Colombetti LG, Rayudu GVS (eds) Radiotracers for medical applications, voll. CRS, Boca Raton, p 1

    Google Scholar 

  • Dezelic G, Dezelic N, Muic N, Pende B (1971) Latex particle agglutination in the immunochemical system human serum albumin-anti human serum albumin rabbit serum. Eur J Biochem. 20: 553–560

    PubMed  CAS  Google Scholar 

  • Dona V (1985) Homogeneous colorimetric enzyme inhibition immunoassay for Cortisol in human serum with Fab anti-glucose 6-phosphate dehydrogenase as a label modulator. J Immunol Methods. 82: 65–75

    PubMed  CAS  Google Scholar 

  • Donabedian RK, Levine TA, Seligson D (1972) Micro-electrolytic iodination of polypeptide hormones for radioimmunoassay. Clin Chim Acta. 36: 517–520

    PubMed  CAS  Google Scholar 

  • Doran DM, Spar I-L (1980) Oxydative iodine monochloride iodination technique. J Immunol. 38: 155–163

    Google Scholar 

  • Doyle MJ, Halsall HB, Heineman W (1982) Heterogeneous immunoassay for serum proteins by differential pulse anodic stripping voltammetry. Anal Chem. 54: 2318–2322

    PubMed  CAS  Google Scholar 

  • Dray F, Andrieu JM, Renaud F (1975) Enzyme immunoassay of progesterone at the pico-gram level using ß-galactosidase. Biochim Biophys Acta. 403: 131–138

    PubMed  CAS  Google Scholar 

  • Edelhoch H (1962) The properties of thyroglobulin. J Biol Chem. 337: 2778–2787

    Google Scholar 

  • Ekeke GI, Exley D, Abukneshar (1979) Immunofluorimetric assay of oestradiol-17ß. J Steroid Biochem. 11: 1597–1600

    CAS  Google Scholar 

  • Ekins RP (1973) The future development of immunoassay. In: Radioimmunoassay and related procedures in medicine, Istambul, IAEA SM-220/204, pp 241–275

    Google Scholar 

  • Ekins RP (1978) Theoretical aspect of saturation analysis. In: Radioimmunoassay and related procedures in medicine, Berlin IAEA-SM-124/105, pp 325–353

    Google Scholar 

  • Ekins RP, Jackson T (1984) Non isotopic immunoassay. An overview. In: Bizollon CA (ed) Monoclonal antibodies and new trends in immunoassays. Elsevier, Amsterdam, pp 149–163

    Google Scholar 

  • Engvall E, Perlmann P (1971) Enzyme-linked immunosorbent assay (ELISA). Quantitative assay of immunoglobulin G. Immunochemistry. 8: 871–874

    PubMed  CAS  Google Scholar 

  • Erlanger BF (1973) Principles and methods for the preparation of drug-protein conjugates for immunological studies. Pharmacol Rev. 25, p 271

    PubMed  CAS  Google Scholar 

  • Eshhar Z, Kim JB, Barnard G, Collins WP, Gilad S, Lindner HR, Kohen F (1981) Use of monoclonal antibody to pregnadiol-3a-glucuronide for the development of a solid- phase chemiluminescence immunoassay. Steroids. 38: 89–109

    PubMed  CAS  Google Scholar 

  • Fagnart OC, Mareschal JC, Cambiaso CL, Masson PL (1985) Particle counting immunoassay (PACIA) of pregnancy-specific fil -glycoprotein, a possible marker of various malignancies and Crohn’s ileitis. Clin Chem. 31: 397–101

    Google Scholar 

  • Finley PR, Williams RJ, Lichti DA (1980) Evaluation of a new homogeneous enzyme inhibitor immunoassay of serum thyroxine with use of bichromatic analyser. Clin Chem. 26: 1723–1726

    PubMed  CAS  Google Scholar 

  • Fraker PJ, Speck JC Jr (1978) Protein and cell membrane iodination with a sparingly soluble chloroamide l,3,4,6-tetrachloro-3a,6a-diphenylglycoluril. Biochem Biophys Res Commun. 80: 849–857

    PubMed  CAS  Google Scholar 

  • Freedlender AE (1968) Practical and theoretical advantages for the use of I125 in radioim-munoassay. In: Margoulies M (ed) Protein and polypeptide hormones, part II. Ex- cerpta Medica, Amsterdam, pp 351–354

    Google Scholar 

  • Fromageot P, Pradelles P, Morgat JL, Levine H (1978) Radioactive labelling of peptide hormones. In: Gupta D, Voelter W (eds) Hypothalamic hormones. Chemistry, physiology and clinical applications. Verlag Chemie, Weinheim, p 59

    Google Scholar 

  • Gandolfi C, Malvano R, Rosa V (1971) Preparation and immunoreactive properties of mono iodinated angiotensin labelled at high specific activity. Biochim Biophys Acta. 251: 254–261

    PubMed  CAS  Google Scholar 

  • Gemmill CL (1955) The apparent ionization constants of the phenolic hydroxyl groups of thyroxine and related compounds. Arch Biochem Biophys. 54: 359–367

    PubMed  CAS  Google Scholar 

  • Geoghegan WD, Ackerman GA (1977) Adsorption of horseradish peroxydase, ovomucoid and anti-immunoglobulin to colloidal gold for the indirect detection of concanavalin A, wheat germ agglutinin and goat anti-human immunoglobulin G on cell surfaces at the electron microscopic level: a new method, theory and application. J Histochem Cy- tochem. 25: 1187–1200

    CAS  Google Scholar 

  • Gibbons I, Skold C, Rowley GL, Ullman EF (1980) Homogeneous enzyme immunoassay for proteins employing ß-galactosidase. Anal Biochem. 102: 167–170

    PubMed  CAS  Google Scholar 

  • Goodman SL, Hodges GM, Trejdosiewicz LK, Livingston DC (1979) Colloidal gold probes - a further evaluation. Scan Electron Microsc. 3: 619–628

    PubMed  Google Scholar 

  • Grange J, Roch AM, Quash GA (1977) Nephelometric assay of antigens and antibodies with latex particles. J Immunol Methods. 18: 365–375

    PubMed  CAS  Google Scholar 

  • Greenwood FC, Hunter WM, Glover JS (1963) The preparation of 131I labelled human growth hormone of high specific radioactivity. Biochem J. 89: 114–123

    PubMed  CAS  Google Scholar 

  • Grouselle D, Tixier-Vidal A, Pradelles P (1982) A new improvement of the sensitivity and specificity of radioimmunoassay for thyroliberin. Application to biological samples. Neuropeptides. 3: 29–4

    PubMed  CAS  Google Scholar 

  • Haimovich J, Sela M (1969) Protein-bacteriophage conjugates: application in detection of antibodies and antigens. Science. 164: 1279–1280

    PubMed  CAS  Google Scholar 

  • Haimovich J, Sela M, Drewdney JM, Batchelor FR (1967) Anti-penicilloyl antibodies: detection with penicilloylated bacteriophage and isolation with a specific immunoadsor- bent. Nature. 214: 1369–1370

    PubMed  CAS  Google Scholar 

  • Handley G, Miller JN, Briges JW (1979) Development of fluorescence immunoassay method of drug analysis. Proc Anal Div Chem Soc. 16: 26–29

    CAS  Google Scholar 

  • Harris CC, Yolken RH, Krokan H, Hsu IC (1979) Ultrasensitive enzymatic radio-immu- noassay: application to detection of cholera toxin and rotavirus. Proc Natl Acad Sci USA. 76: 5336–5339

    PubMed  CAS  Google Scholar 

  • Hassam M, Landon J, Smith DS (1982) A novel non separation fluoroimmunoassay for thyroxine. J Immunoassay. 3: 1–15

    Google Scholar 

  • Hayes CE, Goldstein IJ (1975) Radioiodination of sulphydryl-sensitive proteins. Anal Bio- chem. 67: 580–584

    CAS  Google Scholar 

  • Helmkamp RW, Sears DA (1970) A label for the red cell membrane: diazotized diiodosul- fanilic acid. Int J Appl Radiat Isol. 21: 683–684

    CAS  Google Scholar 

  • Helmkamp RW, Goodland RL, Bale WF, Spar IL, Mutschler LE (1960) High specific ac¬tivity iodination of y-globulin with iodine 131 monochloride. Cancer Res. 20: 1495–1500

    PubMed  CAS  Google Scholar 

  • Helmkamp RW, Contreras MA, Bale WF (1967) 131I-labeling of proteins by the iodine monochloride method. Int J Appl Radiat Isotopes. 18: 737–746

    Google Scholar 

  • Hemmila I (1985) Fluoroimmunoassays and immunofluorometric assays. Clin Chem. 31: 359–370

    PubMed  CAS  Google Scholar 

  • Hemmila I, Dakubu S, Mukkala V-M, Siitari H, Lovgren T (1984) Europium as a label in time-resolved immunofluorometric assays. Anal Biochem. 137: 335–343

    PubMed  CAS  Google Scholar 

  • Hemmings WA, Redshaw M (1975) A biological test of damage caused to IgG by several methods of iodination. Int J Appl Radiat Isotopes. 26: 426–129

    CAS  Google Scholar 

  • Horisberger M, van Lauthan M (1979) Fluorescent colloidal gold: a cytochemical marker for fluorescent and electron microscopy. Histochemistry. 64:115–118 H

    Google Scholar 

  • orrocks DL (1981) Qualitative and quantitative measurements of radioiodines. J Radio-anal Chem. 65: 307–320

    Google Scholar 

  • Hsia JC, Wong LTL, Kalow W (1973) Homogeneous murine myeloma protein 315 and spin labeled DNP as a model system for spin-labeled hapten titration technique and spin immunoassay. J Immunol Methods. 3: 17–24

    CAS  Google Scholar 

  • Hsu IC, Yolken RH, Harris CC (1981) Ultrasensitive enzymatic radioimmunoassay. Methods. 73: 383–394

    CAS  Google Scholar 

  • Hugues WL (1957) The chemistry of iodination. Ann NY Acad Sci. 70: 3–18

    Google Scholar 

  • Hung LT, Fermandjian S, Morgat JL, Fromageot P (1973) Peptide and protein labelling with iodine. Iodine monochloride reaction with aqueous solution of L-tyrosine, L-his- tidine, L-histidine-peptides and its effect on some simple disulfide bridges. J Label Compounds. 10: 3–21

    Google Scholar 

  • Hunter WM (1982) Recent advances in radioimmunoassay and related procedures. In: Radioimmunoassay and related procedures in medicine, Vienna. IAEA-SM-259/101, pp 3–21

    Google Scholar 

  • Hunter WM, Greenwood FC (1962) Preparation of iodine 131 labelled human growth hormone of high specific activity. Nature. 194: 495–496

    PubMed  CAS  Google Scholar 

  • Imagawa M, Yoshitake S, Ishikawa E, Endo Y, Ohtaki S, Kano E, Tsunetoshi Y (1981) Highly sensitive sandwich enzyme immunoassay of human IgE with /?-D-galactosidase from Escherichia coli. Clin Chim Acta. 117: 199–207

    PubMed  CAS  Google Scholar 

  • Imagawa M, Ishikawa E, Yoshitake S, Tanaka K, Kan H, Inada M, Imura H, Kurosaki H, Tachibana S, Takagi M, Nishiura M, Nakazawa N, Ogawa H, Tsunetoshi Y, Naka- jima K (1982) A sensitive and specific sandwich enzyme immunoassay for human thyroid-stimulating hormone. Clin Chim Acta. 126: 227–236

    PubMed  CAS  Google Scholar 

  • Imagawa M, Hashida S, Ishikawa E (1983) A highly sensitive sandwich enzyme immunoassay for insulin in human serum developed using capybara anti-insulin Fab’-horseradish peroxydase conjugate. Anal Lett. 16: 1509–1523

    CAS  Google Scholar 

  • Inoue S, Hashida S, Ishikawa E, Mori T, Imura H, Ogawa H, Ichioka T, Nakajima K (1986) Highly sensitive enzyme immunoassay for human thyroid stimulating hormone (hTSH) in serum using monoclonal anti-TSH ß-subunit IgGl-coated polystyrene balls and polyclonal anti-human chorionic gonadotropin Fab’-horseradish peroxydase conjugate. Anal Lett. 19: 845–861

    CAS  Google Scholar 

  • Ishikawa E, Imagawa M, Hashida S, Yoshitake S, Hamaguchi Y, Ueno T (1983) Enzyme labeling of antibodies and their fragments for enzyme immunoassay and immunohis- tochemical staining. J Immunoassay. 4: 209–327

    PubMed  CAS  Google Scholar 

  • Jablonsky E (1985) The preparation of bacterial luciferase conjugates for immunoassay and application to rubella antibody detection. Anal Biochem. 148: 199–206

    Google Scholar 

  • Jaklitsch AP, Schneider RS, Johannes RJ, Levine JE, Rosenberg GL (1976) Homogeneous enzyme immunoassay for T4 in serum. Clin Chem. 22: 1185

    Google Scholar 

  • Janson JC (1967) Absorption phenomena on Sephadex. J Chromatogr. 28: 12–20

    PubMed  CAS  Google Scholar 

  • Jarvis RF (1979) The future outlook for enzyme immunoassays. Antibiot Chemother. 26: 105–117

    PubMed  CAS  Google Scholar 

  • Jeffcoate SL (1982) Use of 125iodine tracers in steroid radioimmunoassays. In: Gupta D (ed) Radioimmunoassay of steroid hormones. Verlag Chemie, Weinheim, p 209

    Google Scholar 

  • Jiang VW, Krohn KA, Welch MJ (1975) Intramolecular effects of radioiodine decay in O-iodophenol, a model for radioiodinated proteins. J Am Chem Soc. 97: 6551–6556

    PubMed  CAS  Google Scholar 

  • Jirousek L (1981) On the chemical nature of iodinating species. J Radioanal Chem. 65: 139–154

    CAS  Google Scholar 

  • Johannsson A, Stanley CJ, Self CH (1985) A fast highly sensitive enzyme immunoassay system demonstrating benefit of enzyme amplification in clinical chemistry. Clin Chim Acta. 148: 119–124

    PubMed  CAS  Google Scholar 

  • Jolley ME, Stroupe SD, Wang C-HJ, Panas HN, Keegan CL, Schmidt RL, Schwenzer KS (1981) Fluorescent polarization immunoassay I. Monitoring aminoglycoside antibiotics in serum and plasma. Clin Chem. 27: 1190–1197

    PubMed  CAS  Google Scholar 

  • Joronen I, Hopsu-Havu VK, Manninen M, Rinne A, Järvinen M, Halonen P (1986) Detection of low molecular weight cysteine proteinase inhibitors by time-resolved fluoroimmunoassay. J Immunol Methods. 86: 243–247

    PubMed  CAS  Google Scholar 

  • Kaplan LA, Gau N, Stein EA, Fearn JA, Chen IW, Maxon HR, Volle C (1981) Comparison of two nonisotopic immunoassays with a radioimmunoassay for the analysis of serum thyroxine. In: Kaplan LA, Pesce AJ (eds) Nonisotopic alternatives to radioimmunoassay. Dekker, New York, pp 183–189

    Google Scholar 

  • Karnes HT, Gudat JC, O’Donnel CM, Winefordner JD (1981) Double-antibody fluorescence immunoassay of tobramycin. Clin Chem. 27: 249–252

    PubMed  CAS  Google Scholar 

  • Kato K, Hamaguchi Y, Fukui H, Ishikawa E (1975) Enzyme-linked immunoassay II. A simple method for synthesis of the rabbit antibody-ß-D-galactosidase complex and its general applicability. J Biochem. 78: 423–425

    PubMed  CAS  Google Scholar 

  • Kennedy JH, Kricka LJ, Wilding P (1976) Protein-protein coupling reactions and the application of protein conjugates. Clin Chim Acta. 70: 1–31

    PubMed  CAS  Google Scholar 

  • Khanna PL, Ullman EF (1980) 4′5′-dimethoxy-6-carboxy fluorescein. A novel dipole-di- pole coupled fluorescence energy transfer acceptor for fluoroimmuno-assay. Anal Biochem. 108: 156–161

    Google Scholar 

  • Kim JB, Barnard GJ, Collins WP, Kohen F, Lindner HR, Eshhar Z (1982) Measurement of plasma estradiol-17ß by solid-phase chemiluminescence immunoassay. Clin Chem. 28: 1120–1124

    PubMed  CAS  Google Scholar 

  • King TP, Li Y, Kochoumian L (1978) Preparation of protein conjugates via intermolecular disulfide bond. Biochemistry. 17: 1499–1506

    PubMed  CAS  Google Scholar 

  • Kitagawa T, Aikawa T (1976) Enzyme coupled immunoassay of insulin using a novel coupling reagent. J Biochem. 79: 233–236

    PubMed  CAS  Google Scholar 

  • Kjeld JM, Kuku SF, Diamant L, Fräser TR, Joplin GF, Mashiter K (1975) Production and storage of (l25I)thyroxine and (125I) triiodothyronine of high specific activity. Clin Chim Acta. 61: 381–389

    PubMed  CAS  Google Scholar 

  • Kobayashi Y, Yamata M, Watanabe I, Mikai K (1982) A solid-phase fluoroimmunoassay of serum Cortisol. J Steroid Biochem. 16: 521–524

    PubMed  CAS  Google Scholar 

  • Kohen F, Pazzagli M, Kim JB, Lindner HR, Boguslasky RC (1979) An assay procedure for plasma progesterone based on antibody-enhanced chemiluminescence. FEBS Lett. 104: 201–205

    PubMed  CAS  Google Scholar 

  • Kohen F, Kim JB, Barnard G, Lindner HR (1980 a) An assay for urinary estriol-16-gluc-uronide based on antibody-enhanced chemiluminescence. Steroids. 36: 405–119

    Google Scholar 

  • Kohen F, Pazzagli M, Kim JB, Lindner HR (1980 b) An immunoassay for plasma Cortisol based on chemiluminescence. Steroids. 36: 421–437

    Google Scholar 

  • Kolmer JA, Boerner F (1945) Procedure: Lange colloidal gold test. In: Approved laboratory technique. Appleton-Century, New York, p 585

    Google Scholar 

  • Koshland ME, Engelberger FM, Erwin MJ, Gaddone SM (1963) Modification of amino acid residues in anti-ß-azobenzene-arsonic acid antibody during extensive iodination. J Biol Chem. 238: 1343–1348

    PubMed  CAS  Google Scholar 

  • Krohn KA, Welch MJ (1974) Studies of radio-iodinated fibrinogen. II. Lactoperoxydase iodination of fibrinogen and model compounds. Int J Appl Radiat Isot. 25: 315–323

    PubMed  CAS  Google Scholar 

  • Langone J J (1981) Radioiodination by use of the Bolton-Hunter and related reagents. Methods Enzymol. 73: 112

    CAS  Google Scholar 

  • Leute R, Ullman EF, Goldstein A (1972) Spin immunoassay of opiate narcotics in urine and saliva. JAMA. 221: 1231–1234

    PubMed  CAS  Google Scholar 

  • Leuvering JHW, Thai PJHM, Van Der Waart M, Schuurs AHWM (1981) A sol particle agglutination assay for human chorionic gonadotropin. J Immunol Methods. 45: 183–191

    PubMed  CAS  Google Scholar 

  • Leuvering JHW, Tal JHM, Schuurs AHWM (1983) Optimisation of a sol particle immunoassay for human chorionic gonadotropin. J Immunol Methods. 62: 175–184

    PubMed  CAS  Google Scholar 

  • Li TM, Benovic JL, Buckler RT, Burd JF (1981) Homogeneous substrate-labeled fluorescent immunoassay for theophylline in serum. Clin Chem. 27: 22–26

    PubMed  CAS  Google Scholar 

  • Liburdy RP (1979) Antibody induced fluorescence enhancement of an N-(3-pyrene) mal- eimide conjugate of rabbit anti-human immunoglobulin G: quantitation of human IgG. J Immunol Meth. 28: 233–242

    CAS  Google Scholar 

  • Ling N, Leppaluoto J, Vale W (1976) Chemical biochemical and immunological characterization of mono and diiodo thyrotropin releasing factor. Anal Chem. 76: 125–133

    CAS  Google Scholar 

  • Lu-Steffes M, Pittluck GW, Jolley ME, Panas HN, Olive DL, Wang GHJ, Nystrom DD, Keegan CL, Davis TP, Stroupe SD (1982) Fluorescence polarization immunoassay IV. Determination of phenytoin and phenobarbital in human serum and plasma. Clin Chem. 28: 2278–2282

    PubMed  CAS  Google Scholar 

  • Magnusson CGM, Masson PL (1985) Immunoglobulin E assayed after pepsin digestion by an automated and highly sensitive particle counting immunoassay: application to human cord blood. J Allergy Clin Immunol. 75: 513–524

    CAS  Google Scholar 

  • Mani RS (1983) Reactor-produced radionuclides. In: Helus F, Colombetti LG (eds) Radio-nuclides production, vol 2. CRC, Boca Raton, p 1

    Google Scholar 

  • Marchalonis J J (1969) An enzymatic method for the trace iodination of immuno-globulins and other proteins. Biochem J. 113: 299–305

    PubMed  CAS  Google Scholar 

  • Marks V, Mould GP, O’Sullivan MJ, Teale JD (1980) Monitoring of drug disposition by immunoassay. In: Bridges JW, Chasseaud LF (eds) Progress in drug metabolism. Wiley, New York, vol 5, p 255

    Google Scholar 

  • Matkovics B, Rakonczay Z, Rajki SE (1971) Steroids XII - Iodination of aromatic steroids by peroxydases. Steroidologia. 2: 77–79

    PubMed  CAS  Google Scholar 

  • Mc Conahey PJ, Dixon FJ (1980) Radioiodination of proteins by the use of the chloramine T method. Methods Enzymol. 70: 210

    Google Scholar 

  • McFarlane AS (1958) Efficient trace-labelling of protein with iodine. Nature. 182: 53

    PubMed  CAS  Google Scholar 

  • McFarlane AS (1968) In vivo behavior of 131I fibrinogen. J Clin Invest. 42: 346–36

    Google Scholar 

  • Markwell MA (1982) A new solid-state reagent to iodinate proteins. Anal Biochem. 125: 427–32

    PubMed  CAS  Google Scholar 

  • Mersel M, Benenson A, Doljanski F (1976) Lactoperoxydase-catalyzed iodination of surface membrane lipids. Biochem Biophys Res Com. 70: 1166–1171

    PubMed  CAS  Google Scholar 

  • Miles LEM, Hales CN (1973) Immunoradiometric assay procedures: new developments. In: Radioimmunoassay and related procedures in medicine, Istambul. IAEA-SM 124/ 107, pp 483–90

    Google Scholar 

  • Miller JN, Lim CS, Bridges JN (1980) Fluorescamine and fluoresceine as labels in energy transfer immunoassay. Analyst. 105: 91–92

    CAS  Google Scholar 

  • Miyachi Y, Vaitukaitis JL, Nieschlag E, Lipsett MB (1972) Enzymatic radioiodination of gonadotropins. J Clin Endocrinol Metab. 34: 23–28

    PubMed  CAS  Google Scholar 

  • Morgat JL, Hung LT, Fromageot P (1970) Preparation of highly labelled (3H) angiotensin II. Biochim Biophys Acta. 207: 374–376

    PubMed  CAS  Google Scholar 

  • Morrison M (1980) Lactoperoxydase-catalysed iodination as a tool for investigation of proteins. Methods Enzymol. 70: 214

    CAS  Google Scholar 

  • Morton RK (1957) The kinetics of hydrolysis of phenyl phosphate by alkaline phosphatase. Biochem J. 65: 674–682

    PubMed  CAS  Google Scholar 

  • Nakane PK, Kawoi A (1974) Peroxydase-labeled antibody a new method of conjugation. J Histochem Cytochem. 22: 1084–1091

    PubMed  CAS  Google Scholar 

  • Neddermeyer PA, Rogers LB (1968) Gel filtration behavior of inorganic salts. Anal Chem. 40: 755–762

    CAS  Google Scholar 

  • Nelander B (1969) U.V. absorption spectra of complexes between some disulfides and iodine. Acta Chem Scand. 23: 2136–2148

    CAS  Google Scholar 

  • Ngo TT, Lenhoff HM (1980) Enzyme modulators as tools for the development of homogeneous enzyme immunoassay. FEBS Lett. 116: 285–288

    PubMed  CAS  Google Scholar 

  • O’Beirne AJ, Cooper HR (1979) Heterogeneous enzyme immunoassay. J Histochem Cytochem. 27: 1148–1162

    PubMed  Google Scholar 

  • Oellerich M (1984) Enzyme-immunoassay: a review. J Clin Chem Clin Biochem. 22: 895–904

    PubMed  CAS  Google Scholar 

  • Olsson T, Brunius G, Carlsson HE, Thore A (1979) Luminescence immunoassay (L.I.A.): a solid-phase immunoassay monitored by chemiluminescence. J Immunol Methods. 25: 127–135

    PubMed  CAS  Google Scholar 

  • O’Sullivan MJ (1984) Enzyme immunoassay. In: Butt WR (ed) Practical immunoassay. Dekker, New York

    Google Scholar 

  • O’Sullivan MJ, Marks V (1981) Methods for the preparation of enzyme-antibody conjugates for use in enzyme immunoassay. Methods Enzymol. 73: 147–166

    PubMed  Google Scholar 

  • Patel A, Campbell AK (1983) Homogeneous immunoassay based on chemiluminescence energy transfer. Clin Chem. 29: 1604–1608

    PubMed  CAS  Google Scholar 

  • Pazzagli M, Serio M, Munson P, Rodbard D (1982) A chemiluminescent immunoassay (L.I.A.) for testosterone. In: Radioimmunoassay and related procedures in medicine, Vienna. IAEA-SM-25, pp 747–755

    Google Scholar 

  • Pettersson K, Siitari H, Hemmilä I, Soini E, Lövgren T, Hänninen V, Tanner P, Stenman UH (1983) Time-resolved fluoroimmunoassay of human choriogonadotropin. Clin Chem. 29: 60–64

    PubMed  CAS  Google Scholar 

  • Porath J, Flodin P (1959) Gel filtration: a method for desalting and group separation. Nature. 183: 1657–1659

    PubMed  CAS  Google Scholar 

  • Porstmann B, Porstmann T, Nugel E, Evers U (1985) Which of the commonly used marker enzymes gives the best results in colorimetric and fluorometric enzyme immunoassay: horseradish Peroxydase, alkaline phosphatase or ß galactosidase? J Immunol Methods. 79: 27–37

    PubMed  CAS  Google Scholar 

  • Pradelles P (1977) Contribution à l’étude de la conformation du TRF (facteur de libération de l’hormone thyréotrope) par dichroïsme circulaire. Etudes physico-chimiques, marquages radioactifs et application biologiques. Thesis, University of Paris V II

    Google Scholar 

  • Pradelles P, Gros C, Rougeot C, Bepoldin O, Dray F, Llorens-Cortes C, Pollard H, Schwartz JC, Fournier-Zaluski MC, Cracel G, Roques BP (1978) Dosage radioimmu- nologique des enképhalines. In: Radioimmunoassay and related procedure in medicine 1977, vol 2. IAEA-SM 220/67, Vienna, p 495

    Google Scholar 

  • Pradelles P, Grassi J, Maclouf J (1985) Enzyme immunoassays of eicosanoids using acetylcholine-esterase as label: an alternative to radioimmunoassay. Anal Chem. 57: 1170–1173

    PubMed  CAS  Google Scholar 

  • Pressman D, Eisen HN (1950) The zone of localization of antibodies. V. An attempt to saturate antibody-binding site in mouse kidney. J Immunol. 64: 273–279

    PubMed  CAS  Google Scholar 

  • Quash GA, Roch AM, Niveleau A, Grange J, Keolouangkhot T, Huppert J (1978) The preparation of latex particles with covalently bound polyamines, IgG and measles agglutinins and their use in visual agglutination tests. J Immunol Methods. 22: 165–174

    PubMed  CAS  Google Scholar 

  • Rae PA, Schimmer BP (1974) Iodinated derivatives of adrenocorticotropic hormone. J Biol Chem. 249: 5649–5653

    PubMed  CAS  Google Scholar 

  • Ramachandran LK (1956) Protein-iodine interaction. Chem Rev. 56: 199–218

    CAS  Google Scholar 

  • Redshaw MR, Lynch SS (1974) An improved method for the preparation of iodinated antigens for radioimmunoassay. J Endocrinol. 60: 527–528

    PubMed  CAS  Google Scholar 

  • Regoeczi E (1984) Iodine labelled plasma protein. CRS, Boca Rato, p 1

    Google Scholar 

  • Riad-Fahmy D, Read GF, Joyce BG, Walker RF (1981) Steroid immunoassays in endocrinology. In: Yoller A, Bartlett A, Bidwell D (eds) Immunoassays for the 80s. MIT Press, Lancaster, p 205

    Google Scholar 

  • Robbin JL, Hill GA, Carle BN, Calquist JH, Marcus C (1962) Latex agglutination reac¬tions between human chorionic gonadotropin and rabbit antibody. Proc Soc Exp Biol Med. 109: 321–325

    Google Scholar 

  • Rosa U, Scassellati A, Pennisi F, Riccioni N, Giagnoni P, Giordoni R (1964) Labelling of human fibrinogen with 131I by electrolytic iodination. Biochim Biophys Acta. 86: 519–526

    PubMed  CAS  Google Scholar 

  • Rosenberg R, Murray TM (1979) The mechanism of methionine oxidation concomitant with hormone radioiodination. Comparative studies of various oxidants using a simple new method. Biochim Biophys Acta. 584: 261–269

    PubMed  CAS  Google Scholar 

  • Rotman B (1961) Measurement of activity of single molecules of ß-D-galactosidase. Proc Natl Acad Sci USA. 47: 1981–1991

    PubMed  CAS  Google Scholar 

  • Rubenstein KE, Schneider RS, Ullman EF (1972) Homogeneous enzyme-immunoassay. A new immunochemical technique. Biochem Biophys Res Commun. 47: 846–874

    PubMed  CAS  Google Scholar 

  • Rudinger J, Ruegg U (1973) Preparation of N-succinimidyl 3-(4-hydroxyphenyl) propionate. Biochem J. 133: 538–539

    PubMed  CAS  Google Scholar 

  • Rowley GL, Rubenstein KE, Huisjen J, Ullman EF (1975) Mechanism by which antibodies inhibit hapten-malate dehydrogenase conjugates. An enzyme-immunoassay for morphine. J Biol Chem. 250: 3759–3766

    PubMed  CAS  Google Scholar 

  • Salacinski PR, Mc Leau C, Sykes JE, Clement-Jones VY, Lowry PJ (1981) Iodination of proteins, glycoproteins and peptides using a solid-phase oxiding agent, 1,3,4,6-tetra- chloro-3a,6a-diphenyl glycoluril (iodogen). Anal Chem. 117: 136–146

    CAS  Google Scholar 

  • Samols E, Williams HS (1961) Trace labelling of insulin with iodine. Nature. 190: 1211–1212

    PubMed  CAS  Google Scholar 

  • Schall RF, Tenoso HJ (1981) Alternatives to radioimmunoassay: labels and methods. Clin Chem. 27: 1157–1164

    PubMed  CAS  Google Scholar 

  • Schneider RS, Lindquist P, Wong ET, Rubenstein KE, Ullmann EF (1973) Homogeneous enzyme immunoassay for opiate in urine. Clin Chem. 19: 821–825

    PubMed  CAS  Google Scholar 

  • Schroeder HR, Yeager FM (1978) Chemiluminescence yields and detection limit of some isoluminol derivatives in various oxydation systems. Anal Chem. 50: 1114–1120

    CAS  Google Scholar 

  • Schroeder HR, Carrico RJ, Boguslaski RC, Christner JE (1976) Specific binding reactions monitored with ligand-cofactor conjugates and bacterial luciferase. Anal Biochem. 72: 283–292

    PubMed  CAS  Google Scholar 

  • Schroeder HR, Yogelhut PO, Carrico RJ, Boguslaski RC, Buckler RT (1976) Competitive protein binding assay for biotin monitored by chemiluminescence. Anal Chem 48:1933–1937

    Google Scholar 

  • Schroeder HR, Yogelhut PO, Carrico RJ, Boguslaski RC, Buckler RT (1976) Competitive protein binding assay for biotin monitored by chemiluminescence. Anal Chem. 48: 1933–1937

    PubMed  CAS  Google Scholar 

  • Schuurs AHWM, Van Veemen BK (1977) Enzyme-immunoassay. Clin Chim Acta. 81: 1–40

    PubMed  CAS  Google Scholar 

  • Schuurs AHWM, van Veemen BK (1980) Enzyme-immunoassay: a powerfull analytical tool. J Immunoassay. 1: 229–249

    PubMed  CAS  Google Scholar 

  • Sefton BM, Vickus GG, Burge BW (1973) Enzymatic iodination of Sindbis virus proteins. J Virol. 11: 730–735

    PubMed  CAS  Google Scholar 

  • Seidah NS, Dennis M, Corvol P, Rochemont J, Chretien M (1980) A rapid high per-formance liquid chromatography purification method of iodinated polypeptide hormones. Anal Chem. 109: 185–191

    CAS  Google Scholar 

  • Shalev A, Greenberg AH, Mc Alpine PJ (1980) Detection of attograms of antigens by a high-sensitivity enzyme-linked immunosorbent assay (H-S ELISA) using a fluorogenic substrate. J Immunol Meth. 38: 125–139

    CAS  Google Scholar 

  • Shaposhnikov JD, Zerov YP, Ratavitski EA, Ivanov SD, Bobrov YF (1976) In vitro RNA iodination with aid of chloramine T. Anal Chem. 75: 234–240

    CAS  Google Scholar 

  • Shaw EJ, Watson RAA, Landon J, Smith DS (1977) Estimation of serum gentamicin by quenching fluoroimmunoassay. J Clin Pathol. 30: 526–531

    PubMed  CAS  Google Scholar 

  • Shechter Y, Burstein Y, Patchornik A (1975) Selective iodination of methionine residues in proteins. Biochemistry. 14: 4497–503

    PubMed  CAS  Google Scholar 

  • Shima K, Sawazaki N, Tanaka R, Tarui S, Nishikawa M (1975) Effect of an exposure to chloramine T on the immunoreactivity of glucagon. Endocrinology. 96: 1254–1260

    PubMed  CAS  Google Scholar 

  • Siitari H, Hemmila I, Soini E, Lovgren T, Koistinen V (1983) Detection of hepatitis B surface antigen using time-resolved fluoroimmunoassay. Nature. 301: 258–260

    PubMed  CAS  Google Scholar 

  • Sikorav JL, Grassi J, Bon S (1984) Synthesis “in vitro” of precursors of the catalytic sub- units of acetylcholinesterase from Torpedo marmorata and Electrophorus electricus. Eur J Biochem. 145: 519–524

    PubMed  CAS  Google Scholar 

  • Simpson JSA, Campbell AK, Ryall MET, Woodhead JS (1979) A stable chemilumi-nescent-labeled antibody for immunological assay. Nature. 279: 646–647

    PubMed  CAS  Google Scholar 

  • Singer SJ (1959) Preparation of an electron-dense antibody conjugate. Nature. 183: 1523–1524

    PubMed  CAS  Google Scholar 

  • Sinosich MJ, Chard T (1979) Fluoroimmunoassay of alphafoetoprotein (AFP) in amniotic fluid. Ann Clin Biochem. 16: 334–336

    PubMed  CAS  Google Scholar 

  • Smith DS (1977) Enhancement fluoroimmunoassay of thyroxine. FEBS Lett. 77: 25–27

    PubMed  CAS  Google Scholar 

  • Soini E (1984) Pulsed light, time resolved fluorometric immunoassay. In: Bizollon CA (ed) Monoclonal antibodies and new trends in immunoassays. Elsevier, Amsterdam, pp 197–208

    Google Scholar 

  • Sorimachi K, Ui N (1975) Ion exchange chromatography analysis of iodothyronines. Anal Chem. 67: 157–165

    CAS  Google Scholar 

  • Spencer RD (1981) Applications of fluorescent polarization in clinical assays. In: Kaplan LA, Pesce A (eds) Nonisotopic alternatives to radioimmunoassays. Dekker, New York, pp 143–169

    Google Scholar 

  • Spencer RD, Toledo FB, Williams BT, Yoss NL (1973) Design, construction, and two applications for an automated flow-cell polarization fluorometer with digital read out: enzyme-inhibitor (antitrypsin) assay and antigen-antibody (insulin-insulin antiserum) assay. Clin Chem. 19: 838–844

    PubMed  CAS  Google Scholar 

  • Stagg BH, Temperley JM, Rochman H, Morley JS (1970) Iodination and the biological activity of gastrin. Nature. 228: 58–59

    PubMed  CAS  Google Scholar 

  • Stanley CJ, Paris F, Plumb A, Webb A, Johannsson A (1985 a) Enzyme amplification: a new technique for enhancing the speed and sensitivity of enzyme immunoassays. Int Biotechnol. Lab 3: 46–51

    Google Scholar 

  • Stanley CJ, Johansson A, Self CH (1985 b) Enzyme amplification can enhance both the speed and the sensitivity of immunoassays. J Immunol Meth. 83: 89–95

    Google Scholar 

  • Stryer L, Griffith OH (1965) A spin-labelled hapten. Proc Natl Acad Sci USA. 54: 1785–1791

    PubMed  CAS  Google Scholar 

  • Tack BF, Wilder RL (1981) Tritiation of proteins to high specific activity: application to radioimmunoassay. Methods Enzymol. 73: 138

    CAS  Google Scholar 

  • Takayasu S, Maeda M, Tsujii A (1985) Chemiluminescent enzyme immunoassay using D-galactosidase as the label and the bis(2,4,6-trichlorophenyl)oxalate-fluorescent dye system. J Immunol Methods. 83: 317–325

    PubMed  CAS  Google Scholar 

  • Taylor DM (1981) The radiotoxicology of iodine. J Radioanal Chem. 65: 195–208

    CAS  Google Scholar 

  • Terouanne B, Carrie ML, Nicolas JC, Crastes de Paulet A (1986 a) Bioluminescent immunosorbent for rapid immunoassays. Anal Biochem. 154: 118–125

    Google Scholar 

  • Terouanne B, Nicolas JC, Crastes de Paulet A (1986 b) Bioluminescent immunoassay for a-fetoprotein. Anal Biochem. 154: 132–137

    Google Scholar 

  • Thakrar H, Miller JN (1982) New developments in fluorescence immunoassay. Anal Proc. 19: 329–330

    CAS  Google Scholar 

  • Thorell JI, Johansson BG (1971) Enzymatic iodination of polypeptides with 125I to high specific activity. Biochim Biophys Acta. 251: 363–369

    PubMed  CAS  Google Scholar 

  • Tijssen P, Kurstak E (1984) Highly efficient and simple methods for the preparation of peroxydase and active peroxydase-antibody conjugates for enzyme immunoassays. Anal Biochem. 136: 451–457

    PubMed  CAS  Google Scholar 

  • Toivonen E, Hemmila I, Marniemi J, Jorgensen PN, Zeuthen J, Lovgren T (1986) Two-site time-resolved immunofluorometric assay of human insulin. Clin Chem. 32: 637–640

    PubMed  CAS  Google Scholar 

  • Tsang VCW, Hancock K, Maddison SE (1984) Quantitative capacities of glutaraldehyde and sodium m-periodate coupled peroxydase-anti-human IgG conjugates in enzyme- linked immunoassays. J Immunol Meth. 70: 91–100

    CAS  Google Scholar 

  • Tsay YG, Wilson L, Keefe E (1980) Quantitation of serum gentamicin concentration by solid-phase immunofluorescence method. Clin Chem. 26: 1610–1612

    PubMed  CAS  Google Scholar 

  • Tuskynki GP, Knight L, Piperno JR, Walsh PN (1980) A rapid method to removal of (125I) iodide following iodination of protein solutions. Anal Chem. 106: 118–122

    Google Scholar 

  • Ullman EF, Schwarzberg M, Rubenstein KE (1976) Fluorescence excitation transfer immunoassay. A general method for determination of antigens. J Biol Chem. 251: 4172–4178

    PubMed  CAS  Google Scholar 

  • Ullman EF, Yoshida RA, Blakemore JI, Maggio E, Leute R (1979) Mechanism of inhibition of malate dehydrogenase by thyroxine derivatives and reactivation by antibodies. Homogeneous enzyme-immunoassay for thyrosine. Biochim Biophys Acta. 567: 66–74

    PubMed  CAS  Google Scholar 

  • Van Der Waart M, Schuurs AHWM (1976) Enzymoimmunoassay. Towards the development of a radioenzyme-immunoassay (REIA). Z Anal Chem. 279: 142

    Google Scholar 

  • Van Veemen BK, Schuurs AHWM (1971) Immunoassay using antigen-enzyme conjugates. FEBS Lett. 15: 232–236

    Google Scholar 

  • Van Veemen BK, Schuurs AHWM (1972) Immunoassay using hapten-enzyme conjugates. FEBS Lett. 24: 77–81

    Google Scholar 

  • Van Veemen BK, Schuurs AHWM (1975) The influence of heterologous combinations of antiserum and enzyme-labelled estrogen as the characteristics of estrogen enzyme-im- munoassays. Immunochemistry. 12: 667–690

    Google Scholar 

  • Vigny M, Bon S, Massoulie J, Leterrier F (1978) Active-site catalytic efficiency of acetylcholinesterase molecules forms in Electrophorus, Torpedo, rat and chicken. Eur J Bio- chem. 85: 317–323

    CAS  Google Scholar 

  • Vilpo J A, Rasi S, Suvanto E, Vilpo LM (1986) Time-resolved fluoroimmunoassay of 5-methyl-2r-deoxycytidin. Anal Biochem. 154: 436–40

    PubMed  CAS  Google Scholar 

  • Voller A (1982) Application of immunoassays in parasitic diseases. In: Radioimmunoassay and related procedures in medicine, Vienna. IAEA-SM-259/108, p 689

    Google Scholar 

  • Von Schulthess GK, Cohen RJ, Benedek GB (1976) Laser light scattering spectroscopic immunoassay in the agglutination-inhibition mode for human chorionic gonadotropin (hCG) and luteinizing hormone (hLH). Immunochemistry. 13: 963–966

    Google Scholar 

  • Von Schulthess GK, Giglio M, Cannel DS, Benedek GB (1980) Detection of agglutination reactions using anisotropic light scattering: an immunoassay of high sensitivity. Mol Immunol. 17: 81–92

    Google Scholar 

  • Walker WHC (1977) An approach to immunoassay. Clin Chem. 23: 384–402

    PubMed  CAS  Google Scholar 

  • Wannlund J, De Luca MA (1983) Bioluminescent immunoassays. Methods Enzymol. 92: 426–432

    PubMed  CAS  Google Scholar 

  • Weber SG, Purdy WC (1979) Homogeneous voltametric immunoassay: a preliminary study. Anal Litt. 12: 1–9

    CAS  Google Scholar 

  • Weeks I, Beheshti I, Mc Capra F, Campbell AK, Woodhead JS (1983 a) Acridinium esters as high-specific-activity labels in immunoassay. Clin Chem. 29: 1474–1479

    Google Scholar 

  • Weeks I, Campbell AK, Woodhead JS (1983 b) Two site-immunochemiluminometric assay for human fetoprotein. Clin Chem. 29: 1480–1483

    Google Scholar 

  • Weeks I, Sturgess ML, Woodhead JS (1986) Chemiluminescence immunoassay: an overview. Clin Sci. 70: 403–408

    PubMed  CAS  Google Scholar 

  • Wehmeyer KR, Halsall HB, Heineman WR (1982) Electrochemical investigation of hapten-antibody interactions by differential pulse polarography. Clin Chem. 28: 1968–1972

    PubMed  CAS  Google Scholar 

  • Wehmeyer KR, Doyle MJ, Halsall HB, Heineman WR (1983) Immunoassay by electrochemical technique. Methods Enzymol. 92: 432–144

    PubMed  CAS  Google Scholar 

  • Wei R, Almirez R (1975) Spin immunoassay of progesterone. Biochem Biophys Res Commun. 62: 510–516

    PubMed  CAS  Google Scholar 

  • Whitehead TP, Kricka LJ, Carter TJN, Thorpe HG (1979) Analytical luminescence: its potential in the clinical laboratory. Clin Chem. 25: 1531–1546

    PubMed  CAS  Google Scholar 

  • Wilson MB, Nakane PK (1978) Recent development in the periodate method of conjugating horseradish peroxydase (HRPO) to antibodies. In: Knapp W, Holuber K, Wick G (eds) Immuno-fluorescence and related staining techniques. Elsevier, Amsterdam, pp 215–224

    Google Scholar 

  • Wilzbach KE (1957) Tritium-labelling by exposure of organic compounds to tritium gas. J Am Chem Soc. 79: 1013

    CAS  Google Scholar 

  • Wood FT, Wu MN, Gerhart JC (1975) The radioactive labeling of proteins with an iodinated amidination reagent. Anal Chem. 69: 339–349

    CAS  Google Scholar 

  • Wood WG (1984) Luminescence immunoassays: problems and possibilities. J Clin Chem Clin Biochem. 22: 905–918

    PubMed  CAS  Google Scholar 

  • Woodhead JS, Sturgess M, Jones MK, Week I (1984) Chemiluminescent labels in immu¬noassay. In: Bizollon CA (ed) Monoclonal antibodies and new trends in immunoassays. Elsevier, Amsterdam, pp 165–174

    Google Scholar 

  • Worah D, Yeung KK, Ward FE, Carrico RJ (1981) A homogeneous fluorescent immu¬noassay for human immunoglobulin. M Clin Chem. 27: 673–677

    CAS  Google Scholar 

  • Yalow RS (1980) Radioimmunoassay. Am Rev Biophys Bioeng. 9: 327–345

    CAS  Google Scholar 

  • Yalow RS, Berson SA (1968) General principles of radioimmunoassays. In: Hayer RL, Goswitz FA, Murphy BEP (eds) Radioisotopes in medicine: in vitro analysis. Atomic Energy Commission Symposium Series 11, Oak Ridge, p 7

    Google Scholar 

  • Yoshitake S, Yamada Y, Ishikawa E, Masseyeff R (1979) Conjugation of glucose oxydase from Aspergillus niger and rabbit antibodies using N-hydroxysuccinimide ester of N- (4-carboxycyclohexylmethyl)-maleimide. Eur J Biochem. 101: 395–399

    PubMed  CAS  Google Scholar 

  • Zuk RF (1981) Fluorescence-protection immunoassay applied to the measurement of serum proteins. In: Kaplan LA, Pesce A (eds) Nonisotopic alternatives to radioimmunoassay. Dekker, New York, pp 83–95

    Google Scholar 

Download references

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 1987 Springer-Verlag Berlin Heidelberg

About this chapter

Cite this chapter

Grassi, J., Maclouf, J., Pradelles, P. (1987). Radioiodination and Other Labeling Techniques. In: Patrono, C., Peskar, B.A. (eds) Radioimmunoassay in Basic and Clinical Pharmacology. Handbook of Experimental Pharmacology, vol 82. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-71809-0_5

Download citation

  • DOI: https://doi.org/10.1007/978-3-642-71809-0_5

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-71811-3

  • Online ISBN: 978-3-642-71809-0

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics