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Radioimmunoassay of Catecholamines

  • Chapter
Radioimmunoassay in Basic and Clinical Pharmacology

Part of the book series: Handbook of Experimental Pharmacology ((HEP,volume 82))

Abstract

Even nowadays, the determination of catecholamines in biologic samples, especially in plasma and cerebrospinal fluid, is beset with great difficulties. For a long time, fluorometry (particularly the trihydroxyindole method) has been the most widely used method for the determination of catecholamines (Weil-Malherbe 1968; Renzini et al. 1970; Wisser 1970; Miura et al. 1977). The sensitivity of this method is certainly sufficient for the analysis of urine and brain tissue, but not for the determination of unconjugated epinephrine and dopamine in plasma. Gas chromatography, using either flame ionization detection (Lovelady and Foster 1975) or electron capture detection (Imai et al. 1973; Wong et al. 1973; Lhu- Guenot and Maume 1974) has also been introduced to determine catecholamines, but it has not found widespread application. Gas chromatography combined with mass spectrometry (referred to as mass fragmentography) shows high specificity, but requires expensive equipment (Karoum et al. 1972; Koslow et al. 1972; Lhu- Guenot and Maume 1974; Ehrhardt and Schwartz 1978). Moreover, its sen-sitivity is not as high as that of the radioenzymatic methods.

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Authors
  1. E. Knoll
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  2. H. Wisser
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Editors and Affiliations

  1. Institute of Pharmacology, Catholic University of the Sacred Heart, Largo Francesco Vito, 1, I-00168, Rome, Italy

    Carlo Patrono (Professor of Molecular Pharmacology) (Professor of Molecular Pharmacology)

  2. Institute of Pharmacology and Toxicology, Ruhr-University Bochum Im Lottental, P.O. Box 102148, D-4630, Bochum 1, Germany

    Bernhard A. Peskar (Professor of Pharmacology and Toxicology) (Professor of Pharmacology and Toxicology)

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© 1987 Springer-Verlag Berlin Heidelberg

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Knoll, E., Wisser, H. (1987). Radioimmunoassay of Catecholamines. In: Patrono, C., Peskar, B.A. (eds) Radioimmunoassay in Basic and Clinical Pharmacology. Handbook of Experimental Pharmacology, vol 82. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-71809-0_17

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  • DOI: https://doi.org/10.1007/978-3-642-71809-0_17

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-71811-3

  • Online ISBN: 978-3-642-71809-0

  • eBook Packages: Springer Book Archive

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