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Radioimmunoassay in Basic and Clinical Pharmacology pp 351–362Cite as

Radioimmunoassay of Atrial Peptide Blood and Tissue Levels

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Part of the book series: Handbook of Experimental Pharmacology ((HEP,volume 82))

Abstract

The observation (DeBold et al. 1981) that mammalian atria contain a peptide that is both natriuretic and diuretic has led to the discovery of a novel endocrine hormone (atriopeptin, AP) which regulates salt and water metabolism from the kidney (Needleman et al. 1985). Atrial extracts were found to be both heat and acid stable (Debold et al. 1981), and to possess spasmolytic activity on vascular smooth muscle (Currie et al. 1983). These bioassays facilitated the purification and sequence analysis of three related peptides, termed AP I, II, and III (Currie et al. 1984 a). Several peptides of different lengths, but sharing a common core sequence to the APs, have been identified by different laboratories. These include cardionatrin (Flynn et a. 1983), atrial natriuretic factor (ANF) (Thibault et al. 1983), atrial natriuretic polypeptide (ANP) (Kangawa and Matsuo 1984), and auriculin (Atlas et al. 1984). cDNA probes were constructed from the peptide sequence and used to determine the sequence for the AP gene (Yamanaka et al. 1984; Maki et al. 1984; Oikawa et al. 1984; Seidman et al. 1984). The AP gene codes for a 152 amino acid preprohormone which contains a hydrophobic 24 amino acid leader sequence (Fig. 1).

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Authors
  1. M. L. Michener
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  2. D. Schwartz
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  3. M. G. Currie
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  4. D. M. Geller
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  5. P. Needleman
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Editors and Affiliations

  1. Institute of Pharmacology, Catholic University of the Sacred Heart, Largo Francesco Vito, 1, I-00168, Rome, Italy

    Carlo Patrono (Professor of Molecular Pharmacology) (Professor of Molecular Pharmacology)

  2. Institute of Pharmacology and Toxicology, Ruhr-University Bochum Im Lottental, P.O. Box 102148, D-4630, Bochum 1, Germany

    Bernhard A. Peskar (Professor of Pharmacology and Toxicology) (Professor of Pharmacology and Toxicology)

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© 1987 Springer-Verlag Berlin Heidelberg

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Michener, M.L., Schwartz, D., Currie, M.G., Geller, D.M., Needleman, P. (1987). Radioimmunoassay of Atrial Peptide Blood and Tissue Levels. In: Patrono, C., Peskar, B.A. (eds) Radioimmunoassay in Basic and Clinical Pharmacology. Handbook of Experimental Pharmacology, vol 82. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-71809-0_14

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  • DOI: https://doi.org/10.1007/978-3-642-71809-0_14

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-71811-3

  • Online ISBN: 978-3-642-71809-0

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