Radioimmunoassay of Nonpituitary Peptide Hormones

  • G. Nilsson
Part of the Handbook of Experimental Pharmacology book series (HEP, volume 82)


Insulin is a polypeptide consisting of two chains of amino acids linked by two disulfide bridges (Fig. 1). Only minor differences in amino acid composition exist between species (Table 1). These differences are generally not sufficient to influence the biologic activity in heterologous species, but may make the insulin antigenic.


Molecular Weight Form Human Calcitonin Diagnostic Endocrinology 
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  1. Albano JD, Edkins RP, Maritz G, Turner RC (1972) A sensitive, precise radioimmunoassay of serum insulin relying on charcoal separation of bound and free hormone moieties. Acta Endocrinol. 70: 487Google Scholar
  2. Almqvist S, Hjern B, Wâsthed B (1975) The diagnostic value of a radioimmunoassay for parathyroid hormone in human serum. Acta Endocrinol. 78: 493PubMedGoogle Scholar
  3. Alumets J, Hâkansson R, O’Dorisio T, Sjolund K, Sundler F (1978) Is GIP a glucagon cell constituent? Histochemistry. 58: 253PubMedCrossRefGoogle Scholar
  4. Arnaud CD, Tsao HS, Littledike T (1971) Radioimmunoassay of human parathyroid hormone in serum. J Clin Invest. 50: 21PubMedCrossRefGoogle Scholar
  5. Arnaud CD, Goldsmith RS, Sizenore GW, Oldham SB (1973) Studies on the characterization of human parathyroid hormone in hyperthyroid serum; practical considerations. In: Frame B, Parfitt AM, Duncan H (eds) Clinical aspects of metabolic bone disease. Excerpta Medica, Amsterdam, p 207Google Scholar
  6. Ashby JP, Frier BM (1981) Circulating C-peptide: measurement and clinical application. Ann Clin Biochem. 18: 125PubMedGoogle Scholar
  7. Assan R, Rosslin G, Dolais J (1967) Effets sur la glucagonémie des perfusions et ingestions d’acide aminés. Journees annuelles de de Diabétologie de l’Hôtel - Dieu. 7: 25Google Scholar
  8. Aurbach GD, Marx SJ, Spiegel AM (1985) Parathyroid hormone, calcitonin and the calciferols. In: Wilson JD, Foster DW (eds) Textbook of endocrinology. Saunders, Philadelphia, p 1137Google Scholar
  9. Berson SA, Yalow RS (1966) Iodoinsulin used to determine specific activity of iodine-131. Science. 152: 205PubMedCrossRefGoogle Scholar
  10. Berson SA, Yalow RS (1968) Immunochemical heterogeneity of parathyroid hormone in plasma. J Clin Endocr. 28: 1037PubMedCrossRefGoogle Scholar
  11. Berson SA, Yalow RS, Aurbach GD, Potts JT Jr (1963) Immunoassay of bovine and hu¬man parathyroid hormone. Proc Natl Acad Sei USA. 49: 613CrossRefGoogle Scholar
  12. Canterbury JM, Reiss E (1972) Multiple immunoreactive forms of parathyroid hormone in human serum. 140: 1393Google Scholar
  13. Cerasi E, Luft R (1967) Plasma insulin response to glucose infusion in healthy subjects and in diabetes mellitus. Acta Endocrinol. 55: 278PubMedGoogle Scholar
  14. Cerasi E, Luft R, Efendic S (1972) Decreased sensitivity of the pancreatic beta cells to glucose in prediabetic and diabetic subjects. Diabetes. 21: 224PubMedGoogle Scholar
  15. Chesney TMcC, Schofield JG (1969) Studies on the secretion of pancreatic glucagon. Diabetes. 18: 627PubMedGoogle Scholar
  16. Christensen MS (1976) Sensitive radioimmunoassay of parathyroid hormone in human serum using a specific extraction technique. Scand J Clin Lab Invest. 36: 313PubMedCrossRefGoogle Scholar
  17. Christensen MS (1979) Radioimmunoassay of human parathyroid hormone. Dan Med Bull. 26:157Google Scholar
  18. Clark MB, Byfield PHG, Boyd OW, Foster YU (1969) A radioimmunoassay for human calcitonin. Lancet. 2: 74PubMedCrossRefGoogle Scholar
  19. Coffey IW, Nagy CF, Lenusky R, Hansen HI (1974) A radioimmunoassay for plasma insulin using zirconyl phosphate gel. Biochem Med. 9: 54PubMedCrossRefGoogle Scholar
  20. Deftos LJ (1971) Immunoassay for human calcitonin. I. Method. Metabolism. 20: 1122PubMedCrossRefGoogle Scholar
  21. Deftos LJ (1974) Parathyroid hormone. In: Jaffe BM, Behrman HR (eds) Methods of hormone radioimmunoassay. Academic, New York, p 231Google Scholar
  22. Duckworth WC, Kitabchi AE, Heinemann M (1972) Direct measurement of plasma proin- sulin in normal and diabetic subjects. Am J Med. 53: 418PubMedCrossRefGoogle Scholar
  23. Edwards JC, Howell SL, Taylor KW (1970) Radioimmunoassay of glucagon released from isolated guinea pig islets of Langerhans incubated in vitro. Biochem Biophys Acta. 215: 297PubMedCrossRefGoogle Scholar
  24. Faber OK, Binder C (1977) C-Peptide response to glucagon. A test for the residual beta cell function in diabetes mellitus. Diabetes. 26: 605PubMedCrossRefGoogle Scholar
  25. Faloona GR (1973) Radioimmunoassay: Glucagon and GLI. In: Berson SA, Yalow RS (eds) Methods in investigative and diagnostic endocrinology. North-Holland, p 919Google Scholar
  26. Freychet P, Roth J, Neville DM (1971) Monoiodoinsulin: demonstration of its biological activity and binding to fat cells and liver membranes. Biochem Biophys Res Commun. 43: 400PubMedCrossRefGoogle Scholar
  27. Gabbay KH, Deluca K, Fisher JN Jr, Mako ME, Rubenstein AH (1976) Familial hyper-proinsulinemia: an autosomal dominant defect. N Engl J Med. 294: 911PubMedCrossRefGoogle Scholar
  28. Ganong WF (1985) Review of medical physiology, 12th edn. Lange Medical, Los AltosGoogle Scholar
  29. Garcia-Webb P, Bonser A (1979) Decrease in measured C-peptide immunoreactivity on storage. Clin Chem Acta. 95: 139CrossRefGoogle Scholar
  30. Gordon P, Hendricks CM, Roth J (1974) Circulating proinsulin-like component in man - increased proportion in hypoinsulinemia states. Diabetologia. 11: 469CrossRefGoogle Scholar
  31. Habener JF, Potts JT Jr (1977) Chemistry, biosynthesis, secretion and metabolism of parathyroid hormone. In: Aurbach GD (ed) Handbook of physiology, Sect. 7. Endocrinology, vol VII. Williams and Wilkins, Baltimore, p 313Google Scholar
  32. Habener JF, Powell D, Murray TM, Mayer GP, Potts JT Jr (1971) Parathyroid hormone: secretion and metabolism in vivo. Proc Natl Acad Sei. 68: 2986CrossRefGoogle Scholar
  33. Hales CN, Rändle PJ (1963) Immunoassay of insulin with insulin-antibody precipitate. Biochem J. 88: 137PubMedGoogle Scholar
  34. Hamilton CR (1975) Radioimmunoassay of the calcium regulating hormones. In: Rothfeld B (ed) Nuclear medicine in vitro. Lippincott, Philadelphia, p 124Google Scholar
  35. Hazzard WR, Crockford PM, Buchanan KD, Vance JE, Chen R, Williams RH (1968) A double antibody assay for glucagon. Diabetes. 17: 179PubMedGoogle Scholar
  36. Heding LG (1969) The production of glucagon antibodies in rabbits. Hormone Metabol Res. 1: 87CrossRefGoogle Scholar
  37. Heding LG (1971) Radioimmunological determination of pancreatic and gut glucagon in plasma. Diabetologia. 7: 10PubMedCrossRefGoogle Scholar
  38. Heding LG (1972 a) Determination of total serum insulin (IRI) in insulin-treated diabetic patients. Diabetologia. 8:260Google Scholar
  39. Heding LG ( 1972 b) Immunologic properties of pancreatic glucagon: antigenicity and antibody characteristics. In: Lefebvre PJ, Unger RH (eds) Glucagon. Pergamon, Oxford, p 187Google Scholar
  40. Heding LG (1975) Radioimmunological determination of human C-peptide in serum. Dia-betologia. 11: 541CrossRefGoogle Scholar
  41. Heding LG (1978) Insulin, C-peptide and proinsulin in non-diabetics and insulin-treated diabetics: characterization of the proinsulin in insulin-treated diabetics. Diabetes. 27 (Suppl): 178PubMedGoogle Scholar
  42. Herbert V, Lau K, Gottlieb CW, Bleicher CJ (1965) Coated charcoal immunoassay of insulin. J Clin Endocrin Metab. 25: 1375CrossRefGoogle Scholar
  43. Heynen G, Franchimont P (1974) Human calcitonin radioimmunoassay in normal and pathological conditions. Eur J Clin Invest. 4: 213PubMedGoogle Scholar
  44. Hoekstra JBL, van Rijn HJM, Erkelens DN, Thijssen JHH (1982) C-peptide. Diabetes Care 5: 438CrossRefGoogle Scholar
  45. Horwitz DL, Starr JI, Marks ME, Blackard WG, Rubenstein AH (1975) Proinsulin, insulin and C-peptide concentrations in human portal and peripheral blood. J Clin Invest. 55: 1278PubMedCrossRefGoogle Scholar
  46. Horwitz DL, Rubenstein AH, Steiner DF (1978) Proinsulin and C-peptide in diabetes. Med Clin North Am. 62: 723PubMedGoogle Scholar
  47. Hunter WM, Greenwood FC (1962) Preparation of iodine-131 -labelled growth hormone of high specific activity. Nature. 194: 495PubMedCrossRefGoogle Scholar
  48. Kagan A (1975) Radioimmunoassay of insulin. Semin Nucl Med. 5: 183PubMedCrossRefGoogle Scholar
  49. Karesen R, Tronier B, Aune S (1980) Immunoreactive glucagon, insulin and C-peptide in man after resection of the pancreas and total pancreatectomy. Am J Surg. 140: 272PubMedCrossRefGoogle Scholar
  50. Kuzuya H, Blix P, Horwitz DL, Steiner DF, Rubenstein AH (1977) Determination of free and total insulin and C-peptide in insulin-treated diabetics. Diabetes. 26: 22PubMedCrossRefGoogle Scholar
  51. Lafferty FW (1966) Pseudohyperparathyroidism. Medicine (Baltimore) 45: 247CrossRefGoogle Scholar
  52. Larsson LI, Moody AJ (1980) Glicentin and gastric inhibitory polypeptide immunoreactivity in endocrine cells of the gut and pancreas. J Histochem Cytochem. 28: 925PubMedCrossRefGoogle Scholar
  53. Leclercq-Meyer V, Miahle P, Malaisse WJ (1970) Une méthode de dosage radioimmuno- logique du glucagon comportant une separation par le charbon-dextran. Diabetologia. 6: 121PubMedCrossRefGoogle Scholar
  54. Levy LJ, Spergel G, Bleicher S J (1970) Proc Endocrin Soc. 134 (Abstr)Google Scholar
  55. Mayer GP, Keaton JA, Hurst JG, Habener JF (1977) Effects of plasma calcium concentration on the relative proportion of hormone and carboxyl fragment in parathyroid venous blood. Endocrinology. 100s: 234 [Abstr] 355Google Scholar
  56. Melani F, Rubenstein AH, Oyer PE, Steiner DF (1970) Identification of proinsulin and C- peptide in human serum by specific immunoassay. Proc Natl Acad Sei USA. 67: 148CrossRefGoogle Scholar
  57. Müller WA, Faloona GR, Aguilar-Parada E, Unger RH (1970) Abnormal alpha-cell function in diabetes. Response to carbohydrate and protein ingestion. N Engl J Med. 283: 109PubMedCrossRefGoogle Scholar
  58. Nakagawa S, Nakayama H, Sasaki T, Yoshino K, Ying Yu Y, Shinozaki K, Aoki S, Mashimo K (1973) A simple method for the determination of serum free insulin levels in insulin-treated patients. Diabetes. 22: 590PubMedGoogle Scholar
  59. Nilsson G, Unväs-Wallensten K (1974) Effect of teasing, sham feeding and feeding on plasma insulin concentrations in dogs. In: Radioimmunoassay: methodology and applications in physiology and in clinical studies. Commemorative issue for Solomon A. Berson. Horm Metab Res 91Google Scholar
  60. Nilsson G, Uvnäs-Wallensten K (1977) Effect of teasing and sham feeding on plasma glucagon concentration in dogs. Acta Physiol Scand. 100: 298PubMedCrossRefGoogle Scholar
  61. Nonaka K, Foa (1969) A simplified glucagon immunoassay and its use in a study of incubated pancreatic islets. Proc Soc Exp Biol Med. 130: 330Google Scholar
  62. Oyer PE, Cho S, Peterson JD, Steiner DF (1971) Studies on human proinsulin. J Biol Chem. 246: 1375PubMedGoogle Scholar
  63. Palmieri GMA, Yalow RS, Berson SA (1971) Adsorbent techniques for the separation of antibody-bound from free peptide hormones in radioimmunoassay. Horm Metab Res. 3: 301PubMedCrossRefGoogle Scholar
  64. Paloyan E (1967) Recent developments in the early diagnosis of hyperparathyroidism. Surg Clin North Am. 47: 61PubMedGoogle Scholar
  65. Paloyan D, Paloyan E, Worobec R, Ernst K, Deininger E, Harper PV (1966) Serum glucagon levels in experimental acute pancreatitis in the dog. Surg Forum. 17: 348PubMedGoogle Scholar
  66. Reiss E, Canterbury JM (1968) Experience with radioimmunoassay of parathyroid hormone in human sera. Proc Soc Exp Biol Med. 128: 501Google Scholar
  67. Rodman JS, Sherwood LM (1978) Disorders of mineral metabolism in malignancy. In: Avioli LA, Krane SM (eds) Metabolic bone disease. Academic, New York, vol II, p 578Google Scholar
  68. Roth J, Gordon P, Pastan I (1968) “Big insulin”: a new component of plasma insulin detected by immunoassay. Proc Natl Acad Sci USA. 61: 138Google Scholar
  69. Rubenstein AH, Welbourne WP, Maks M, Melani F, Steiner DF (1970) Comparative immunology of bovine, porcine and human proinsulins and C-peptides. Diabetes. 19: 546PubMedGoogle Scholar
  70. Rubenstein AH, Melani F, Steiner DF (1973) Proinsulin and C-peptide in human serum. In: Berson SA, Yalow RS (eds) Methods in investigative and diagnostic endocrinology. North-Holland, Amsterdam, p 870Google Scholar
  71. Safrit HF, Young CW (1977) Factitious hypoglycemia. N Engl J Med. 298: 515Google Scholar
  72. Sandler R, Horwitz DL, Rubenstein AH, Kuzuya H (1975) Hypoglycemia and endogenous hyperinsulinism complicating diabetes mellitus. Am J Med. 59: 730PubMedCrossRefGoogle Scholar
  73. Schopman W, Hackeng HL, Leguin RM (1970) A radioimmunoassay for parathyroid hormone in man. I. Development of a radioimmunoassay for bovine PTH. Acta Endocrinol. 63: 643PubMedGoogle Scholar
  74. Service FJ, Horwitz DL, Rubenstein AH (1977) C-peptide suppression test for insulinoma. J Lab Clin Med. 90: 180PubMedGoogle Scholar
  75. Service FJ, Rubenstein AH, Horwitz DL (1975) C-peptide analysis in diagnosis of factitial hypoglycemia in an insulin-dependent diabetic. Mayo Clin Proc. 50: 697Google Scholar
  76. Silva OL, Snider RH, Becker KL (1974) A radioimmunoassay of calcitonin in human plasma. Clin Chem. 20: 337PubMedGoogle Scholar
  77. Smith LF (1966) Species variation in the amino acid sequence of insulin. Am J Med. 40: 662PubMedCrossRefGoogle Scholar
  78. Smith PH, Merchant FW, Johnson DG, Fujimoto WY, Williams RH (1977) Immunocy- tochemical localization of a gastric inhibitory polypeptide-like material within A-cells of the endocrine pancreas. Am J Anat. 149: 585PubMedCrossRefGoogle Scholar
  79. Soeldner JS, Slone D (1965) Critical variables in the radioimmunoassay of serum insulin using the double antibody technique. Diabetes. 19: 1Google Scholar
  80. Steiner DF (1972) Proinsulin. Triangle. 11: 51PubMedGoogle Scholar
  81. Stellon A, Townell NH (1979) C-peptide assay for factitious hyperinsulinism. Lancet. 2: 148PubMedCrossRefGoogle Scholar
  82. Stewart AF, Horst R, Deftos LJ, Cadman EC, Lang R, Broadus AE (1980) Biochemical evaluation of patients with cancer-associated hypercalcemia. New Engl J Med. 303: 1377PubMedCrossRefGoogle Scholar
  83. Sundby F, Jacobsen H, Moody AJ (1976) Purification and characterization of a protein from porcine gut with glucagon-like immunoreactivity. Horm Metab Res. 8: 366PubMedCrossRefGoogle Scholar
  84. Tanaka M, Abe K, Adachi I, Yamaguchi K, Miyakawa S, Hirakawa H, Kumasaka S (1975) Radioimmunoassay specific for amino (N) and carboxyl (C) terminal portion of parathyroid hormone. Endocrinol Jpn. 22: 471PubMedCrossRefGoogle Scholar
  85. Tashjian AH, Vollkel EF (1974) Human calcitonin: application of affinity chromatography. In: Jaffe BM, Behrman HR (eds) Methods of hormone radioimmunossay. Academic, New York, p 199Google Scholar
  86. Thorell JI, Larsson SM (1978) Radioimmunoassay and related techniques. Mosby, Saint Louis, Appendix 17Google Scholar
  87. Turner RC, Heding LG (1977) Plasma proinsulin, C-peptide and insulin in diagnostic sup-pression tests for insulinomas. Diabetologia. 13: 351CrossRefGoogle Scholar
  88. Turner RC, Johnson PC (1973) Suppression of insulin release by fish-insulin-induced hypoglycemia with reference to the diagnosis of insulinomas. Lancet. 2: 1483CrossRefGoogle Scholar
  89. Unger RH (1973) Clinical evaluation of glucagon excess and deficiency. In: Berson SA, Yalow RS (eds) Methods in investigative and diagnostic endocrinology. North-Holland, Amsterdam, p 937Google Scholar
  90. Unger RH, Eisentraut AM, McCall MS, Madison LL (1961) Glucagon antibodies and an immunoassay for glucagon. J Clin Invest. 40: 1280PubMedCrossRefGoogle Scholar
  91. Unger RH, Eisentraut AM, Madison LL (1963) The effects of total starvation upon levels of circulating glucagon and insulin. J Clin Invest. 42: 1031PubMedCrossRefGoogle Scholar
  92. Unger RH, Ohneda E, Aguilar-Parada E, Eisentraut AM (1970) Studies of pancreatic alfa cell function in normal and diabetic subjects. J Clin Invest. 49: 837PubMedCrossRefGoogle Scholar
  93. Velasco CA, Cole HS, Damerino-Davalos PA (1974) Radioimmunoassay of insulin. Clin Chem 20: 700PubMedGoogle Scholar
  94. Weinges KF (1968) Labelled proteins. In: Tracer studies. European Atomic Energy Community Euroatom, p 271Google Scholar
  95. Yalow RS, Berson SA (1960) Immunoassay of endogenous plasma insulin in man. J Clin Invest. 39: 1157PubMedCrossRefGoogle Scholar
  96. Yalow RS, Berson SA (1973) Radioimmunoassay. In: Berson SA, Yalow RS (eds) Methods in investigative and diagnostic endocrinology. North-Holland, Amsterdam, p 864Google Scholar

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  • G. Nilsson

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