Dynamics of Structural Changes in Hemoglobin

  • Eric R. Henry
  • James Hofrichter
  • William A. Eaton
Conference paper
Part of the Springer Series in Biophysics book series (BIOPHYSICS, volume 1)

Abstract

Upon dissociation of oxygen or carbon monoxide hemoglobin undergoes a series of conformational changes that are responsible for its cooperative behavior. X-ray structural studies show that these changes include a displacement of the iron from the heme plane and a tilt of the proximal histidine associated with a shift in the F helix (1–3). This shift destabilizes the intersubunit bonding between α β dimers, and after dissociation of one or two ligands the strain at the interface is sufficient to cause a transition from the R quaternary structure to the T quaternary structure.

Keywords

Cobalt Recombination Carbon Monoxide Histidine Porphyrin 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Perutz, M. F. (1979) Ann. Rev. Biochem. 48, 327–386.CrossRefGoogle Scholar
  2. 2.
    Baldwin, J. and Chothia, C. (1979) J. Mol. Biol. 129, 175–220.PubMedCrossRefGoogle Scholar
  3. 3.
    Gelin, B. R., Lee, A. W-M. and Karplus, M. (1983) J. Mol. Biol. 171, 489–559.PubMedCrossRefGoogle Scholar
  4. 4.
    Martin, J. L., Migus, A., Poyart, C., Lecarpentier, Y., Astier, R., and Antonetti, A. (1983) Proc. Natl. Acad. Sci. USA 80, 173–177.CrossRefGoogle Scholar
  5. 5.
    Cornelius, P. A., Steele, A. W., Chernoff, D. A., and Hochstrasser, R. M. (1981) Proc. Natl. Acad. Sci. USA 78, 7526–7529.CrossRefGoogle Scholar
  6. 6.
    Greene, B. I., Hochstrasser, R. M., Weisman, R. B., and Eaton, W. A. (1978) Proc. Natl. Acad. Sci. USA 75, 5255–5259.CrossRefGoogle Scholar
  7. 7.
    Chernoff, D. A., Hochstrasser, R. M., and Steele, A. W. (1980) Proc. Natl. Acad. Sci. USA 77, 5606–5610.CrossRefGoogle Scholar
  8. 8.
    Terner, J., Stong, J. D., Spiro, T. P., Nagumo, M., Nicol, M., and El-Sayed, M. A. (1981) Proc. Natl. Acad. Sci. USA 78, 1313–1317.CrossRefGoogle Scholar
  9. 9.
    Findsen, E. W., Friedman, J. M., Ondrias, M. R., and Simon, S. R. (1985) Science 229, 661–665.PubMedCrossRefGoogle Scholar
  10. 10.
    Friedman, J. M., Scott, T. W., Fisanick, G. J., Simon, S. R., Findsen, E. W., Ondrias, M. R., and MacDonald, V. W. (1985) Science 229, 187–190.PubMedCrossRefGoogle Scholar
  11. 11.
    Ansari, A., Di Iorio, E. E., Dlott, D. D., Frauenfelder, H., Iben, E. T., Langer, P., Roder, H., Sauke, T. B., Shyamsunder, E. (1986) Biochemistry (in press).Google Scholar
  12. 12.
    Hofrichter, J., Sommer, J. H., Henry, E. R., and Eaton, W. A. (1983) Proc. Natl. Acad. Sci. USA 80, 2235–2239.CrossRefGoogle Scholar
  13. 13.
    Henry, E. R., Hofrichter, J., Sommer, J. H., and Eaton, W. A. (1983) in Photochemistry and Photobiology, Vol. II (Zewail, A. H., ed.), Harwood Academic Publishers, New York pp. 791–810.Google Scholar
  14. 14.
    Hofrichter J., Henry, E. R., Sommer, J. H., Deutsch, R., Ikeda-Saito, M. Yonetani, T., and Eaton, W. A. (1985) Biochemistry 24, 2667–2679.PubMedCrossRefGoogle Scholar
  15. 15.
    Friedman, J. M. (1985) Science 228, 1273–1280.PubMedCrossRefGoogle Scholar
  16. 16.
    Ferrone, F. A., Martino, A. J., and Basak, S. (1985) Biophys. J. 48, 269–282.PubMedCrossRefGoogle Scholar
  17. 17.
    Henry, E. R., Levitt, M., Eaton, W. A. (1985) Proc. Natl. Acad. Sci. USA 82, 2034–2038.CrossRefGoogle Scholar
  18. 18.
    Henry, E. R., Eaton, W. A., and Hochstrasser, R. M. (1986) Proc. Natl. Acad. Sci. (in press).Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1987

Authors and Affiliations

  • Eric R. Henry
    • 1
  • James Hofrichter
    • 1
  • William A. Eaton
    • 1
  1. 1.Laboratory of Chemical PhysicsNIDDK, National Institutes of HealthBethesdaUSA

Personalised recommendations