Imino-Proton Exchange and Base-Pair Kinetics of Nucleic Acids
Imino proton exchange can be monitored in nmr by its effect on longitudinal or transverse relaxation, and by saturation transfer (1–3): It is inhibited when the imino-proton is hydrogeṅ-bonded in a base-pair. Exchange takes place when the pair opens (4). It is catalyzed by general bases (5) such as imidazole, ammonia, tris, phosphate, etc. If the rate of catalysis in the open state is large enough, proton exchange occurs for each base-pair opening event. Only then is the time for proton exchange equal to the proton life-time (opening-limited exchange) (6).
KeywordsEnthalpy Catalysis Imino
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