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Imino-Proton Exchange and Base-Pair Kinetics of Nucleic Acids

  • M. Guéron
  • M. Kochoyan
  • J. L. Leroy
Conference paper
Part of the Springer Series in Biophysics book series (BIOPHYSICS, volume 1)

Abstract

Imino proton exchange can be monitored in nmr by its effect on longitudinal or transverse relaxation, and by saturation transfer (1–3): It is inhibited when the imino-proton is hydrogeṅ-bonded in a base-pair. Exchange takes place when the pair opens (4). It is catalyzed by general bases (5) such as imidazole, ammonia, tris, phosphate, etc. If the rate of catalysis in the open state is large enough, proton exchange occurs for each base-pair opening event. Only then is the time for proton exchange equal to the proton life-time (opening-limited exchange) (6).

Keywords

Catalyst Concentration Exchange Time Saturation Transfer Intermolecular Association Acceptor Stem 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Crothers, D.M., Cole, P.E., Hilbers, C.W. and Shulman, R.G. (1974). J. Mol. Biol. 87, 63–88.PubMedCrossRefGoogle Scholar
  2. 2.
    Johnston, P.D. and Redfield, A.G. (1978). Nucl. Acids. Res. 5, 3913–3327.PubMedCrossRefGoogle Scholar
  3. 3.
    Johnston, P.D. and Redfield, A.G. (1977). Nucl. Acids Res. 4, 3599–3615.PubMedCrossRefGoogle Scholar
  4. 4.
    Teitelbaum, H. and Englander, S.W. (1975a). J. Mol. Biol. 92, 55–78.PubMedCrossRefGoogle Scholar
  5. 5.
    Fritzsche, H, Kan, L.S., and Ts’o, P.O.P. (1981). Biochemistry 20, 6118–6122.PubMedCrossRefGoogle Scholar
  6. 6.
    Englander, S.W., and Kallenbach, N.R. (1983). Quart. Rev. Biophys. 16, 521–655.CrossRefGoogle Scholar
  7. 7.
    Leroy, J.L., Broseta, D., and Guéron, M. (1985) J. Mol. Biol 184, 165–178.PubMedCrossRefGoogle Scholar
  8. 8.
    Leroy, J.L., Bolo, N., Figueroa, N., Plateau, P., and Guéron, M. (1985). J. Biomol. Struct. and Dynam. 2, 915–939.Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1987

Authors and Affiliations

  • M. Guéron
    • 1
  • M. Kochoyan
    • 1
  • J. L. Leroy
    • 1
  1. 1.Groupe de BiophysiqueEcole PolytechniquePalaiseauFrance

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