Molecular Cloning and Physiological Analysis of the Start Gene cdc25 in Budding Yeast
There is increasing interest for the yeast Saccharomyces cevevisiae in studies on the regulatory mechanisms of cell proliferation, since it is particularly well characterized from the biochemical and genetic point of view and furthermore the recombinant DNA techniques have now greatly enhanced the power of classical yeast genetics (Hinnen et al. 1978). S. cerevisiae presents a major cell cycle control function in G1 , in the regulatory area called Start (Hartwell 1974, Hartwell et al. 1974). At Start an yeast cell integrates many intracellular and environmental signals, and then it is committed to continue proliferation or switched to differentiative pathways such as sporulation, conjugation, or to entry in stationary phase. Growth to a critical cell size appears to be required among other conditions to traverse Start and to be committed to DNA replication and cell division (Nurse 1981, Pringle and Hartwell 1981).
KeywordsRestrictive Temperature CDC25 Gene cAMP Metabolism CDC25 Locus CDC25 mRNA
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