Skip to main content
SpringerLink
Log in

Promoters and Operon Structure of the Nodulation Region of the Rhizobium Leguminosarum Symbiosis Plasmid pRL1JI

  • Conference paper
Recognition in Microbe-Plant Symbiotic and Pathogenic Interactions

Part of the book series: NATO ASI Series ((ASIH,volume 4))

Abstract

Bacteria of the genus Rhizobium, which are able to establish a symbiosis with leguminous plants, invade the roots of their hosts where they induce the formation of nodules in which they fix atmospheric nitrogen. Each Rhizobium strain has only a narrow range of host plants on which it is successfully able to establish a symbiosis. In fast-growing Rhizobia, which include R. leguminosarum with peas and vetches as their hosts, R. trifolii with clovers as their hosts, and R. meliloti with alfalfa as a host, many genes involved in nodulation (nod) and nitrogen fixation (nif) are localized on large plasmids designated symbiosis (Sym) plasmids (Johnston et al 1978; Hooykaas et al 1981; Banfalvi et al 1981; Rosenberg et al 1981). The nod genes, which constitute only a relatively small portion of the Sym plasmid (Downie et al 1983; Schofield et al 1984), appear to be of prime importance in the determination of the host-specificity.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Bagdasarian M, Bagdasarian MM, Coleman S and Timmis KN (1979) New vectors for cloning in Pseudomonas. In: Timmis KN and Pühler A (eds). Plasmids of medical, environmental and commercial importance. Elsevier/North Holland 1979 Amsterdam: 411-422.

    Google Scholar 

  • Banfalvi Z, Sakanyan V, Koncz C, Kiss A, Dusha I and Kondorosi A (1981) Location of nodulation and nitrogen fixation genes on a high molecular weight plasmid of R. meliloti. Molec Gen Genet 184: 318–325.

    PubMed  CAS  Google Scholar 

  • Ditta G, Stanfield S, Corbin D and Helinski DR (1980) Broad host range DNA cloning system for gram-negative bacteria: construction of a gene bank of Rhizobium meliloti. Proc Natl Acad Sci U.S.A. 77 7347–7351.

    Article  PubMed  CAS  Google Scholar 

  • Djordjevic MA, Schofield PR and Rolfe BG (1985a) Tn5 mutagenesis of Rhizobium trifolii host-specific nodulation genes result in mutants with altered host-range ability. Mol Gen Genet 200: 463–471.

    Article  CAS  Google Scholar 

  • Djordjevic MA, Schofield PR, Ridge RW, Morrison NA, Bassam BJ, Plazinski J, Watson JM and Rolfe BG (1985b) Rhizobium nodulation genes involved in root hair (Hac) are functionally conserved. Plant Mol Biol 4: 147–160.

    Article  CAS  Google Scholar 

  • Downie JA, Hombrecher G, Ma Q-S, Knight CD, Wells B and Johnston AWB (1983) Cloned nodulation genes of Rhizobium leguminosarum determine host-range specificity. Molec Gen Genet 190: 359–365.

    Article  CAS  Google Scholar 

  • Downie J, Knight CD, Johnston AWB and Rossen L (1985) Identification of genes and gene products involved in the nodulation of peas by Rhizobium leguminosarum. Molec Gen Genet 198: 255–262.

    Article  CAS  Google Scholar 

  • Egelhoff TT, Fisher RF, Jacobs TW, Mulligan JT and Long SR (1985) Nucleotide sequence of Rhizobium meliloti 1021 nodulation genes: nodD is read divergently from nodABC. DNA 4: 241–248.

    Article  PubMed  CAS  Google Scholar 

  • Fisher RF, Tu JK and Long SR (1985) Conserved nodulation genes in Rhizobium meliloti and Rhizobium trifolii. Appl Env Microbiol 49: 1432–1435.

    CAS  Google Scholar 

  • Hirsch PR, van Montagu M, Johnston AWB, Brewin NJ and Schell J (1980) Physical identification of bacteriocinogenic nodulation and other plasmids in strains of Rhizobium leguminosarum. J Gen Microbiol 120: 403–412.

    Google Scholar 

  • Hooykaas PJJ, van Brussel AAN, den Dulk-Ras H, van Slogteren GMS and Schilperoort RA (1981) Sym plasmid of Rhizobium trifolii expressed in different Rhizobium species and Agrobacterium tumefaciens. Nature 291: 351–353.

    Article  CAS  Google Scholar 

  • Hooykaas PJJ, Snijdewint FGM and Schilperoort RA (1982) Identification of the Sym plasmid of Rhizobium leguminosarum strain 1001 and its transfer to and expression in other Rhizobia and Agrobacterium tumefaciens. Plasmid 8: 73–82.

    Article  PubMed  CAS  Google Scholar 

  • Innes RW, Kuempel PL, Plazinski J, Canter-Cremers H, Rolfe BG and Djordjevic MA (1985) Plant factors induce expression of nodulation and host-range genes in Rhizobium trifolii. Molec Gen Genet 201: 426–432.

    Article  CAS  Google Scholar 

  • Jacobs TW, Egelhoff TT and Long SR (1985) Physical and genetic map of a Rhizobium meliloti nodulation gene region and nucleotide sequence of nodC. J Bact 162: 469–476.

    PubMed  CAS  Google Scholar 

  • Johnston AWB, Beynon JL, Buchanan-Wollaston AV, Setchell SM, Hirsch PR and Beringer JE (1978) High frequency of transfer of nodulating ability between strains and species of Rhizobium. Nature 276: 635–636.

    Article  Google Scholar 

  • Kondorosi E, Banfalvi Z and Kondorosi A (1984) Physical and genetic analysis of a symbiotic region of Rhizobium meliloti: identification of nodulation genes. Mol Gen Genet 193: 445–452.

    Article  CAS  Google Scholar 

  • Legocki RP, Yun AC and Szalay AA (1984) Expression of β-galactosidase controlled by a nitrogenase promoter in stem nodules of Aeschynomene scabra. Proc Natl Acad Sci U.S.A. 81: 5806–5810.

    Article  PubMed  CAS  Google Scholar 

  • Long SR, Buikema WJ and Ausubel RM (1982) Cloning of Rhizobium meliloti nodulation genes by direct complementation of nod - mutants. Nature 298: 485–488.

    Article  CAS  Google Scholar 

  • Marsh JL, Erfle M and Wijkes EJ (1984) The pIC plasmid and phage vectors with versatile cloning sites for recombinant selection by insertional inactivation. Gene 32: 481–485.

    Article  PubMed  CAS  Google Scholar 

  • Mulligan JT and Long SR (1985) Induction of Rhizobium meliloti nodC expression by plant exudates requires nodD. Proc Natl Acad Sci U.S.A. 82: 6609–6613.

    Article  PubMed  CAS  Google Scholar 

  • Okker RJH, Spaink H, Wijffelman CA, van Brussel AAN and Lugtenberg B (1986) Regulation of bacterial genes involved in bacterium-plant interactions by plant signal molecules. In: Bailey JA (ed) Biology and molecular biology of plant pathogen interactions. Springer Verlag, in press.

    Google Scholar 

  • Rosenberg C, Boistard P, Dénarié J and Casse-Delhart F (1981) Genes controling early and late functions in symbiosis are located on a mega-plasmid in Rhizobium meliloti. Molec Gen Genet 184: 326–333.

    PubMed  CAS  Google Scholar 

  • Rossen L, Johnston AWB and Downie JA (1984) DNA sequence of the Rhizobium leguminosarum nodulation genes nodAB and C required for root hair curling. Nucl Acid Res 12: 9497–9508.

    Article  CAS  Google Scholar 

  • Rossen L, Shearman CA, Johnston AWB and Downie JA (1985) The nodD gene of Rhizobium leguminosarum is autoregulatory and in the presence of plant exudates induces the nodA,B,C genes. EMBO J 4: 3369–3373.

    PubMed  CAS  Google Scholar 

  • Rostas K, Kondorosi E, Horvath B, Simoncsits A and Kondorosi A (1986) Conservation of extended promoter regions of nodulation genes in Rhizobium. Proc Natl Acad Sci U.S.A. 83: 1757–1761.

    Article  PubMed  CAS  Google Scholar 

  • Sanger F, Nicklen S and Coulson AR (1977) DNA sequencing with chain terminating inhibitors. Proc Natl Acad Sci U.S.A. 74: 5463–5467.

    Article  PubMed  CAS  Google Scholar 

  • Schmidhauser J and Helinsky DR (1985) Regions of broad host—range plasmid RK2 involved in replication and stable maintenance in nine species of gram-negative bacteria. J Bact 164: 446–455.

    PubMed  CAS  Google Scholar 

  • Schofield PR, Djordjevic MA, Rolfe BG, Shine J and Watson JM (1983) A molecular linkage map of nitrogenase and nodulation genes in Rhizobium trifolii. Mol Gen Genet 192: 459–465.

    Article  CAS  Google Scholar 

  • Schofield PR, Ridge RW, Rolfe BG, Shine J and Watson JM (1984) Host-specific nodulation is encoded on a 14 kb fragment in Rhizobium trifolii. Plant Mol Biol 3: 3–11.

    Article  CAS  Google Scholar 

  • Schofield PR and Watson JM (1986) DNA sequence of Rhizobium trifolii nodulation genes reveals a reiterated and potentially regulatory sequence. Nucl Acids Res 14: 2891–2903.

    Article  PubMed  CAS  Google Scholar 

  • Scott KF (1986) Conserved nodulation genes from the non-legume symbiont Bradyrhizobium sp. (Parasponia). Nucl Acids Res 14: 2905–2919.

    Article  PubMed  CAS  Google Scholar 

  • Shearman CA, Rossen L, Johnston AWB and Downie JA (1986) The Rhizobium leguminosarum nodulation gene nodF encodes a polypeptide similar to acyl-carrier protein and is regulated by nodD plus a factor in pea root exudate. EMBO J. 5: 647–652.

    PubMed  CAS  Google Scholar 

  • Török I, Kondorosi E, Stepkowski T, Pósfai J and Kondorosi A (1984) Nucleotide sequence of Rhizobium meliloti nodulation genes. Nucl Acids Res 12: 9509–9523.

    Article  PubMed  Google Scholar 

  • Van Brussel AAN, Zaat SAJ, Canter-Cremers HCJ, Wijffelman CA, Pees E, Tak T and Lugtenberg BJJ (1986) Role of plant root exudates and Sym plasmid localized nodulation genes in the synthesis by Rhizobium leguminosarum of Tsr factor, which causes thick and short roots on common vetch. J Bact 165: 517–522.

    Google Scholar 

  • Wijffelman CA, Pees E, van Brussel AAN, Okker RJH and Lugtenberg BJJ (1985) Genetic and functional analysis of the nodulation region of the Rhizobium leguminosarum Sym plasmid pRLlJI. Arch Microbiol 143: 225–232.

    Article  CAS  Google Scholar 

  • Winarno R and Lie TA (1979) Competition between Rhizobium strains in nodule formation: interactions between nodulating and non-nodulating strains. Plant and Soil 51: 135–142.

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Plant Molecular Biology, University of Leiden, Nonnensteeg 3, 2311 VJ, Leiden, The Netherlands

    Herman P. Spaink, Robert J. H. Okker, Carel A. Wijffelman, Elly Pees & Ben Lugtenberg

Authors
  1. Herman P. Spaink
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Robert J. H. Okker
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Carel A. Wijffelman
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Elly Pees
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Ben Lugtenberg
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Department of Plant Molecular Biology, University of Leiden, Leiden, The Netherlands

    Ben Lugtenberg

Rights and permissions

Reprints and permissions

Copyright information

© 1986 Springer-Verlag Berlin Heidelberg

About this paper

Cite this paper

Spaink, H.P., Okker, R.J.H., Wijffelman, C.A., Pees, E., Lugtenberg, B. (1986). Promoters and Operon Structure of the Nodulation Region of the Rhizobium Leguminosarum Symbiosis Plasmid pRL1JI. In: Lugtenberg, B. (eds) Recognition in Microbe-Plant Symbiotic and Pathogenic Interactions. NATO ASI Series, vol 4. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-71652-2_5

Download citation

  • DOI: https://doi.org/10.1007/978-3-642-71652-2_5

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-71654-6

  • Online ISBN: 978-3-642-71652-2

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation