Abstract
Until very recently (Eilert et al 1986), transfer of cells to alkaloid production medium was the only method available to induce indole alkaloid accumulation and enzymes involved in their biosynthesis. However, the slow rates of enzyme induction observed presented difficulties in performing detailed biochemical investigations. Eilert et al (1986) reported that elicitor treatment of Catharanthus cell suspension cultures stimulated production of indole alkaloids within 12–24 h after treatment and the alkaloid profile obtained was qualitatively and quantitatively identical to that obtained by cultivation of cells in alkaloid production medium.
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References
Eilert U, Constabel F, Kurz, W (1986) Elicitor-stimulation of Monoterpene Indole Alkaloid Formation in Suspension Cultures of Catharanthus roseus. J Plant Physiol (in press).
Sasse F, Buchholz M and Berlin J (1983) Site of Action of Growth Inhibitory Analogues in Catharanthus roseus Cell Suspension Cultures. Z Naturforsch 38c: 910–915.
Mizukami H, Nordlov H, Lee S, Scott A (1979) Purification and Properties of Strictosidine Synthase (an enzyme condensing tryptamine and secologanin) from Catharanthus roseus cultured cells. Biochemistry 18: 3760–3763.
Noe W, Berlin J (1986) Induction of de-novo synthesis of tryptophan decarboxylase in cell suspension cultures of Catharanthus roseus. Planta 166: 500–504.
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© 1986 Springer-Verlag Berlin Heidelberg
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Eilert, U., DeLuca, V., Constabel, F., Kurz, W.G.W. (1986). Elicitation of Indole Alkaloid Biosynthesis in Periwinkle. In: Lugtenberg, B. (eds) Recognition in Microbe-Plant Symbiotic and Pathogenic Interactions. NATO ASI Series, vol 4. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-71652-2_34
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DOI: https://doi.org/10.1007/978-3-642-71652-2_34
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