Elicitation of Indole Alkaloid Biosynthesis in Periwinkle

  • U. Eilert
  • V. DeLuca
  • F. Constabel
  • W. G. W. Kurz
Conference paper
Part of the NATO ASI Series book series (volume 4)

Abstract

Until very recently (Eilert et al 1986), transfer of cells to alkaloid production medium was the only method available to induce indole alkaloid accumulation and enzymes involved in their biosynthesis. However, the slow rates of enzyme induction observed presented difficulties in performing detailed biochemical investigations. Eilert et al (1986) reported that elicitor treatment of Catharanthus cell suspension cultures stimulated production of indole alkaloids within 12–24 h after treatment and the alkaloid profile obtained was qualitatively and quantitatively identical to that obtained by cultivation of cells in alkaloid production medium.

Keywords

Tryptophan Alkaloid Indole Monoterpene Tryptamine 

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References

  1. Eilert U, Constabel F, Kurz, W (1986) Elicitor-stimulation of Monoterpene Indole Alkaloid Formation in Suspension Cultures of Catharanthus roseus. J Plant Physiol (in press).Google Scholar
  2. Sasse F, Buchholz M and Berlin J (1983) Site of Action of Growth Inhibitory Analogues in Catharanthus roseus Cell Suspension Cultures. Z Naturforsch 38c: 910–915.Google Scholar
  3. Mizukami H, Nordlov H, Lee S, Scott A (1979) Purification and Properties of Strictosidine Synthase (an enzyme condensing tryptamine and secologanin) from Catharanthus roseus cultured cells. Biochemistry 18: 3760–3763.PubMedCrossRefGoogle Scholar
  4. Noe W, Berlin J (1986) Induction of de-novo synthesis of tryptophan decarboxylase in cell suspension cultures of Catharanthus roseus. Planta 166: 500–504.CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1986

Authors and Affiliations

  • U. Eilert
    • 2
  • V. DeLuca
    • 1
  • F. Constabel
    • 1
  • W. G. W. Kurz
    • 1
  1. 1.Plant Biotechnology InstituteNational Research Council of CanadaSaskatoonCanada
  2. 2.Inst. Pharmazeut. BiologieBraunschweigGermany

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