A Study of Surface Interactions Between Rhizobium Bacteroids and the Peribacteroid Membrane using Monoclonal Antibodies
As a way of supplementing the other techniques for research into the molecular biology of the Rhizobium-legume symbiosis, we have chosen to apply the technology of monoclonal antibodies to the problem of surface interactions between the endosymbiotic bacteroid and the encircling plant-derived peribacteroid membrane. Using monoclonal antibodies in conjunction with the recently developed techniques for immunogold electron microscopy (Robertson et al 1985), there are three kinds of biological questions that we want to ask. Firstly, how is this interaction set up? In other words, how does the Rhizobium cell become taken up by the plant cell plasma membrane; what governs subsequent divisions of the intracellular bacterium and the concomitant division of the encircling peribacteroid membrane (Robertson & Lyttleton 1984); and what physical interactions occur between bacteroid and peribacteroid membrane surfaces which might control morphogenesis? A second class of question relates to membrane transport phenomena — what kinds of molecules are transported across the bacteroid outer membrane and the peribacteroid membrane and how is this achieved? The third class of question relates to the ultimate breakdown of the symbiosis — nodule senescence — which is in some way related to the nitrogen status of the whole plant.
KeywordsFusion Experiment Immunoaffinity Column Immunogold Electron Microscopy Peribacteroid Membrane Plant Glycoprotein
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- Bradley DJ, Butcher GW, Galfre G, Wood EA, Brewin NJ. (1986) Physical association between the peribacteroid membrane and lipopolysaccharide from the bacteroid outer membrane in Rhizobium-infected pea root nodule cells. J Cell Biol. In the press.Google Scholar
- Brewin NJ, Wood EA, Larkins AP, Galfre G, Butcher GW. (1986) Analysis of lipopolysaccharide from root nodule bacteroids of R. leguminosarum using monoclonal antibodies. J Gen Microbiol 132 (in press).Google Scholar
- Robertson JG, Wells B, Brewin NJ, Williams MA. (1985). Immunogold localisation of cellular consituents in the legume-Rhizobium symbiosis. In: BJ Mifiln (ed) Oxford Reviews of Plant Molecular and Cell Biology, Vol. 2, pp.69-89.Google Scholar