cAMP-Dependent Phosphorylation of the Nicotinic Acetylcholine Receptor: Characterization of the Protein Kinase in Torpedo Electric Organ, Lack of Correlation in Myotubes between Increased Intracellular Levels of cAMP and Influx of Monovalent Ions, Block of Influx by Forskolin Independently of cAMP Levels

  • E. Heilbronn
  • H. Eriksson
  • J. Häggblad
Conference paper
Part of the NATO ASI Series book series (volume 3)

Abstract

The nicotinic acetylcholine receptor (nAChR) from Torpedo electroplax is phosphorylated by at least three different protein kinases (PK) (review: Heilbronn et al, 1985), one of which is cAMP-dependent (Huganir & Greengard, 1983; Zavoiko et al, 1984; Eriksson et al, 1985). Experiments performed in vitro with exogenous catalytic PK subunit (Huganir & Greengard, 1983) or with endogenous PK and receptor (Eriksson et al, 1986) show that cAMP-dependent PK mainly phosphorylates the and the subunits of the receptor. From the known primary structure of Torpedo nAChR (Noda et al, 1983) it can be deduced that the serine residues suggested to face the inside of the electrocyte may be the target of this phosphorylation (Vandlen et al, 1979; Huganir et al, 1983). Preliminary sequence studies on the phosphorylated S subunit of the electric organ receptor (Liljeqvist et al, unpublished) as well as work with a synthetic peptide (Safran, personal communication) suggest that only one of the serines is phosphorylated.

Keywords

Permeability Serotonin Serine Neurol Cholin 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Corbin, J.D., Keely, S.L. & Park, C.R. 1975. J. Biol. Chem. 250: 218.PubMedGoogle Scholar
  2. Eldefrawi, A.T., Miller, E.R., Murphy, D.L. & Eldefrawi, M.E. 1982. Mol. Pharmacol. 22: 72.PubMedGoogle Scholar
  3. Eriksson, H., Salmonsson, R., Liljeqvist, G. & Heilbronn, E. 1985. In: Dynamics of cholinergic Function (Hanin, ed.) Raven Press, New York. In press.Google Scholar
  4. Eriksson, H., Salmonsson, R., Liljegvist, G. & Heilbronn, E. 1986. J. Neurochem. In press. et al 1986Google Scholar
  5. Fox, G.Q. & Richardson, G.P. 1979. J. Comp. Neurol. 185: 293.PubMedCrossRefGoogle Scholar
  6. Häggblad, J., Eriksson, H. & Heilbronn, E. 1985. Acta Pharmacol. et toxicol. 57: 317.CrossRefGoogle Scholar
  7. Häggblad, J., Hedlund, B. & Heilbronn, E. 1986. Manuscript to be submitted to Eur. J. Pharmacol.Google Scholar
  8. Heilbronn, E., Eriksson, H. & Salmonsson R. 1985. In: Molecular Basis of Nerve Activity (Changeux, Hucho, Maelicke, Neumann, eds.) Walter de Gruyter & Co. Berlin, New York. p 237.Google Scholar
  9. Huganir, R.L. & Greengard, P. 1983. Proc. Natl. Acad. Sci. ( USA ) 80: 1130.CrossRefGoogle Scholar
  10. Huganir, R.L., Albert, K.A. & Greengard, P. 1983. Soc. Neurosci. Abstr. 9: 578.Google Scholar
  11. Kassis, K., Hagman, J., Fishman, P.H., Chang, P.P. & Moss, J. 1982. J. Biol. Chem. 257: 12148.PubMedGoogle Scholar
  12. Noda, M., Takahashi, H., Tanabe, T., Toyosato, M., Kikyotami, S., Furutami, Y., Hirose, T., Takashima, H., Inayama, S., Miyata, T. & Numa, S. 1983. Nature (Lond). 320: 528.CrossRefGoogle Scholar
  13. Reuter, H. 1983. Nature (Lond). 301: 569.CrossRefGoogle Scholar
  14. Saitoh, T. & Changeux, J.-P. 1981. Proc. Natl. Acad. Sci. ( USA ) 78: 4430.CrossRefGoogle Scholar
  15. Seamon, K.B., Padgett, W. & Daly, J.W. 1981. Proc. Natl. Acad. Sci. ( USA ) 78: 3363.CrossRefGoogle Scholar
  16. Siegelbaum, S.A., Camardo, J.S. & Kandel, E.R. 1982. Nature (Lond). 299: 413.CrossRefGoogle Scholar
  17. Smith, S.B., LaPorte, D., Builder, S., Beavo, J.A. & Krebs, E.G. 1979. Fed. Proc. 38: 465.Google Scholar
  18. Vandlen, R.L., Wu, W.C.-S., Eisenbach, J.C. & Raftery, M.A. 1979. Biochemistry 18: 1845.PubMedCrossRefGoogle Scholar
  19. Walum, E. 1982. Toxicology 25: 67.PubMedCrossRefGoogle Scholar
  20. Zavoico, G.B., Comerci, C., Subers, E., Egan, J.J., Huang, C.-K., Feinstein, M. & Smilowitz, H. 1984 Biochem. Biophys. Acta 770: 225.PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1986

Authors and Affiliations

  • E. Heilbronn
    • 1
  • H. Eriksson
    • 1
  • J. Häggblad
    • 1
  1. 1.Unit of Neurochemistry and NeurotoxicologyUniversity of StockholmStockholmSweden

Personalised recommendations