Abstract
The glutathione S-transferases (GST) are a major, multi-gene, group of detoxication proteins. A rapid, two-step purification, that employs S-hex- ylglutathione affinity chromatography and hydroxyapatite HPLC, is described for the GST isoenzymes in mouse liver. The major hepatic forms comprise Yf(Mr 24500)-, Ya(Mr 26000)- and Yb(Mr 27000)-type subunits. The isoeletric points of the Yf, Ya and Yb dimers are 8.6, 9.2 and 7.8–8.2 respectively. Immunochemical experiments showed the purified mouse subunits cross-reacted with antisera raised against rat GST subunits that possessed equivalent molecular masses; the subunit sizes of the distinct subunit types are conserved between these two species. The catalytic activities of the purified mouse GST subunits are described. In common with other species the mouse GST are subject to tissue-specific expression. However, only mouse liver and not rat, guinea pig, hamster or human livers express the Yf polypeptide. The significance of this difference is unclear but, in the rat, the Yf subunit can serve as a marker for pre-neoplastic hepatocellular carcinogenesis.
Preview
Unable to display preview. Download preview PDF.
References
Agius C, Gidari AS (1985) Effect of streptozotocin on the glutathione S-transferases of mouse liver cytosol. Biochem Pharmacol 34: 811–819
Boyland E, Chasseaud LF (1969) The role of glutathione and glutathione S-transferases in mercapturic acid biosynthesis. Adv Enzymol 32: 173–219
Chasseaud LF (1976) Conjugation with glutathione and mercapturic acid excretion. In: Arias IM, Jakoby WB (eds) Glutathione: Metabolism and Function. Raven Press, New York, pp 77–114
Coles B, Meyer DJ, Ketterer B, Stanton CA, Garner RC (1985) Studies on the detoxication of micro- somally-activated aflatoxin Bt by glutathione and glutathione transferases in vitro. Carcinogenesis 6: 693–697
Farber E (1984) Pre-cancerous steps in carcinogenesis: their physiological adaptive nature. Biochim Biophys Acta 738: 171–180
Frey AB, Friedberg T, Oesch F, Kreibich G (1983) Studies on the subunit composition of rat liver glu¬tathione S-transferases. J Biol Chem 258: 11321–11325
Glatt H, Friedberg T, Grover PL, Sims P, Oesch F (1983) Inactivation of a diol-epoxide and a K-region epoxide with high efficiency by glutathione transferase X. Cancer Res 43: 5713–5717
Habig WH, Jakoby WB (1981) Assays for differentiation of glutathione S-transferases. Meth Enzymol 77: 398–405
Hayes JD (1984) Purification and characterization of glutathione S-transferases P, S and N. Isolation from rat liver of YbxYn protein, the existence of which was predicted by subunit hybridization in vitro. Biochem J 224: 839–852
Hayes JD (1986) Purification and physical characterization of glutathione S-transferase K. Differential use of S-hexyl-glutathione and glutathione affinity matrices to isolate a novel glutathione S-trans¬ferase from rat liver. Biochem J 233: 789–798
Hayes JD, Strange RC, Percy-Robb IW (1981) A study of the structures of the YaYa and YaYc glu¬tathione S-transferases from rat liver cytosol. Evidence that the Ya monomer is responsible for lithocholate-binding activity. Biochem J 197: 491–502
Hayes JD, Chalmers J (1983) Bile acid inhibition of basic and neutral glutathione S-transferases in rat liver. Biochem J 215: 581–588
Hayes JD, Mantle TJ (1986) Use of immuno-blot techniques to discriminate between the glutathione S-transferase Yf, Yk, Ya, Yn/Yb and Yc subunits and to study their distribution in extra-hepatic tissues. Evidence for three immunochemically distinct groups of transferase in the rat. Biochem J 233: 779–788
Jakobson I, Askelof P, Warholm M, Mannervik B (1977) A steady-state-kinetic random mechanism for glutathione transferase A from rat liver. Eur J Biochem 77: 253–262
Jakoby WB (1978) The glutathione S-transferases: A group of multifunctional detoxification proteins. Adv Enzymol 46: 383–114
Jensson H, Eriksson LC, Mannervik B (1985) Selective expression of glutathione transferase isoen¬zymes in chemically-induced pre-neoplastic rat hepatocyte nodules. FEBS Lett 187: 115–120
Jernstrom B, Martinez M, Meyer DJ, Ketterer B (1985) Glutathione conjugation of the carcinogenic and mutagenic electrophile (+)-7,8,8a-dihydroxy-9a,10a-oxy-7,8,9,10-tetra hydrobenzo(a)pyrene catalyzed by purified rat liver glutathione transferases. Carcinogenesis 6: 85–89
Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (Lond) 227: 680–685
Lee C-Y, Johnson L, Cox RH, McKinney JD, Lee S-M (1981) Mouse liver glutathione S-transferases. Biochemical and immunochemical characterization. J Biol Chem 256: 8110–8116
Lee C-YG (1982) Biochemical and immunochemical analysis of an abundant form of glutathione S-transferase in mouse testis. Mol Cell Biochem 49: 161–168
Mannervik B (1985) The isoenzymes of glutathione transferase. Adv Enzymol 57: 357–417
Mannervik B, Guthenberg C (1981) Glutathione transferase (human placenta). Meth Enzymol 77: 231–235
Meyer DJ, Beale D, Tan KH, Coles B, Ketterer B (1985) Glutathione transferases in primary rat he¬patomas: the isolation of a form with GSH peroxidase activity. FEBS Lett 184: 139–143
Pearson WR, Windle J J, Morrow JF, Benson AM, Talalay P (1983) Increased synthesis of glutathione S-transferases in response to anticarcinogenic antioxidants. Cloning and measurement of messenger RNA. J Biol Chem 258: 2052–2062
Satoh K, Kitahara A, Soma Y, Inaba Y, Hatayama I, Sato K (1985) Purification, induction and dis¬tribution of placental glutathione transferase. A new marker enzyme for preneoplastic cells in the rat chemical hepatocarcinogenesis. Proc Natl Acad Sci USA 82: 3964–3968
Simons PC, Vander Jagt DL (1977) Purification of glutathione S-transferases from human liver by glu-tathione-afifinity chromatography. Anal Biochem 82: 334–341
Smith GJ, Ohl VS, Litwack G (1977) Ligandin, the glutathione S-transferases and chemically-induced hepatocarcinogenesis: a review. Cancer Res 37: 8–14
Sparnins VL, Venegas PL, Wattenberg LW (1982) Glutathione S-transferase activity: enhancement by compounds inhibiting chemical carcinogenesis and by dietary constituents. J Natl Cancer Inst 68: 493–496
Towbin H, Staehlin T, Gordon J (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications. Proc Natl Acad Sci USA 76: 4350–4354
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1987 Springer-Verlag
About this paper
Cite this paper
Hayes, J.D., Coulthwaite, R.E., Stockman, P.K., Hussey, A.J., Mantle, T.J., Wolf, C.R. (1987). Glutathione S-Transferase Subunits in the Mouse and Their Catalytic Activities Towards Reactive Electrophiles. In: Chambers, P.L., Henschler, D., Oesch, F. (eds) Mouse Liver Tumors. Archives of Toxicology, vol 10. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-71617-1_11
Download citation
DOI: https://doi.org/10.1007/978-3-642-71617-1_11
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-17124-9
Online ISBN: 978-3-642-71617-1
eBook Packages: Springer Book Archive