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Recent Improvements in Microscopy Towards Analysis of Transcriptionally Active Genes and Translocation of RNP-Complexes

  • Conference paper
Nucleocytoplasmic Transport

Abstract

Whereas insight into the structural and functional organization of defined cytoplasmic compartments has increased dramatically in the past years (see, e.g. Cold,Spring Harbor Symp. Vol.XLVI, 1982), predominantly by application of antibody labelling techniques, insight into nuclear chromatin organization is still rather limited. Among the obvious reasons for these limitations are (i) the general absence of defined — e.g. membrane surrounded — intranuclear domains, and (ii) the high compaction of most transcriptionally active genes to very dense, small complexes. As a consequence most high magnification analyses of nuclear structures and RNP transport phenomena did require up to now — the application of electron microscopical (EM) techniques. This generally does not allow a direct visualization of in vivo occurring transport phenomena.

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References

  • Agutter, P.S. (1982) In: The Nuclear Envelope and the Nuclear Matrix, pp. 91–109, Alan Liss, New York

    Google Scholar 

  • Allen, R.D. (1985) Ann. Rev. Biophys. Biophys. Chem. 14, 265–290

    Article  CAS  Google Scholar 

  • Allen, R.D., David, G.B. and Normarski, G. (1969) Z. wiss. Mikrosk. Mikrotechn. 69, 193–221

    CAS  Google Scholar 

  • Allen, R.D. and Allen, N.S. (1983) J. Microscopy 129, 3–17

    Article  CAS  Google Scholar 

  • Angelier, LSI., Hemon, D. and Bouteille, M. (1976) Exp. Cell. Res. 100, 389–393

    Article  PubMed  CAS  Google Scholar 

  • Arechaga, J. and Bahr, G.F. (1985) In: Nuclear Envelope Structure and RNA maturation, pp. 23–50, Alan Liss, New York

    Google Scholar 

  • Bard, F., Bourgeois, C.A., Costagliola, D. and Bouteille, M. (1985) Biol. Cell 54, 135–142

    PubMed  CAS  Google Scholar 

  • Beermann, W. (1963) In: 13. Mosbacher Kolloquium, pp. 64–100, Springe Berlin

    Google Scholar 

  • Callan, H.G. and Lloyd, L. (1960) Phil. Trans. B 243, 135–219

    Article  Google Scholar 

  • Clawson, G.A. and Smuckler, E.A. (1982) In: The Nuclear Envelope and the Nuclear Matrix, pp. 271–278, Alan Liss, New York

    Google Scholar 

  • Cold Spring Harbor Syp. Quant. Biol. (1982) Organization of the Cytoplasm, vol. XLVI

    Google Scholar 

  • Daneholt, B., Andersson, K., Björkroth, B. and Lamb, M.M. (1982) Eur. J. Cell Biol. 26, 325–332

    PubMed  CAS  Google Scholar 

  • Dubochet, J., Morel, C., Lebleu, B. and Herzberg, M. (1973) Eur. J. Biochem. 36, 465–472

    Article  PubMed  CAS  Google Scholar 

  • Edström, J.E., Rydlander, L. and Thyberg, J. (1983) Eur. J. Cell Biol 29, 281–287

    PubMed  Google Scholar 

  • Fakan, S. and Puvion, E. (1980) Int. Rev. Cytol. 65, 255–299

    Article  PubMed  CAS  Google Scholar 

  • Feldherr, K.M. (1980) Cell Tissue Res. 205, 157–162

    Article  PubMed  CAS  Google Scholar 

  • Ford, P.J. (1979) In: Maternal Effects in Development (D.R. Newth & M Balls, eds.), pp. 81–110, Cambridge University Press, Cambridge

    Google Scholar 

  • Franke, W.W. (1974) Int. Rev. Cytol. 39, 71–236

    Google Scholar 

  • Franke, W.W. and Scheer, U. (1974) Symp. Soc. Exp. Biol. XXVIII, 249–278

    Google Scholar 

  • Franke, W.W., Scheer, U., Spring, H., Trendelenburg, M.F. and Zentgraf, H. (1979) In: The Cell Nucleus (H. Busch, ed.), vol. 7, pp. 49–95, Academic Press, New York

    Google Scholar 

  • Franke, W.W., Scheer,, Krohne, G. and Jarasch, E.D. (1981) J.Cell Biol. 91, 39–50

    Article  CAS  Google Scholar 

  • Gall, J.G. (1954) J. Morphol. 94, 283–351

    Article  CAS  Google Scholar 

  • Gall, J.G. (1966) In: Methods in Cell Physiology (D.M. Prescott, ed.) vol. II, pp. 37 - 60, Academic Press, New York

    Google Scholar 

  • Gundlach, H. (1979) In: Moderne Untersuchungsmethoden in der Zytologie (S. Witte, R. Ruch, eds.) pp. 27–35, Witzstrock, Baden-Baden

    Google Scholar 

  • Hadjiolov, A. (1985) Cell Biol. Monogr. Vol. 12, pp. 1–268, Springer Wien

    Google Scholar 

  • Hofmann, A., Laier, A. and Trendelenburg, M.F. (1985) In: Molekular- und Zellbiologie (N. Blin, M.F. Trendelenburg & R.E. Schmidt, eds.) pp. 144–158, Springer, Berlin

    Google Scholar 

  • Mathis, D., Oudet, P. and Chambon, P. (1980) Progr. Nucleic Acid Res. Mol. Biol. 24, 1–55

    Article  CAS  Google Scholar 

  • Matsuura, S., Morimoto, T., Tashiro, Y., Higashinakagawa, T. and Muramatsu, M. (1974) J. Cell Biol. 63, 629–640

    Article  PubMed  CAS  Google Scholar 

  • Maul, G.G. (1977) Int. Rev. Cytol. Suppl. 6, 75–86

    PubMed  CAS  Google Scholar 

  • Maul, G.G. (1982) In: The Nuclear Envelope and the Nuclear Matrix, pp. 1–11, Alan Liss, New York

    Google Scholar 

  • Miller, O.L. (1981) J. Cell. Biol. 91, 15–27

    Article  CAS  Google Scholar 

  • Miller, O.L. and Beatty, B.R. (1969) Science 164, 955–957

    Article  PubMed  Google Scholar 

  • Moreno Diaz de la Espina, S., Franke, W.W., Krohne, G., Trendelenburg, M.F., Grund, C. and Scheer, U. (1982) Eur. J. Cell Biol. 27, 141–150

    PubMed  CAS  Google Scholar 

  • Olins, A.L., Olins, D.E. and Franke, W.W. (1980) Eur. J. Cell Biol. 22, 714–723

    PubMed  CAS  Google Scholar 

  • Ross, K.F.A. (1967) In: Phase Contrast and Interference Contrast Microscopy for Cell Biologists, Arnold, London

    Google Scholar 

  • Skoglund, U., Andersson, K., Bjorkroth, B., Lamb, M.M. and Daneholt, B. (1983) Cell 34, 847–855

    Article  PubMed  CAS  Google Scholar 

  • Spring, H. and Franke, W.W. (1981) Eur. J. Cell Biol. 24, 298–308

    PubMed  CAS  Google Scholar 

  • Stevens, B.J. and Swift, H. (1966) J. Cell Biol. 31, 55–77

    Article  PubMed  CAS  Google Scholar 

  • Trendelenburg, M.F. (1983) Hum. Genet. 63, 197–215

    Article  PubMed  CAS  Google Scholar 

  • Trendelenburg, M.F., Scheer, U. and Franke, W.W. (1973) Nature New Biol. 245, 167–170

    PubMed  CAS  Google Scholar 

  • Trendelenburg, M.F., Scheer, U., Zentgraf, H. and Franke, W.W. (1976) J. Mol. Biol. 108, 453–470

    Google Scholar 

  • Trendelenburg, M.F., Franke, W.W. and Scheer, U. (1977) Differentiation 7, 133–158

    Article  PubMed  CAS  Google Scholar 

  • Tröster, H., Spring, H., Meissner, B., Schultz, P., Oudet, P. and Trendelenburg, M.F. (1985) Chromosoma 91, 151–163

    Article  PubMed  Google Scholar 

  • Unwin, P.N.T. and Milligan, R.A. (1982) J. Cell Biol. 93, 63–75

    Article  PubMed  CAS  Google Scholar 

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Author information

Authors and Affiliations

  1. Institute of Cell and Tumor Biology, USA

    M. Trendelenburg, B. Meissner & H. Tröster

  2. German Cancer Research Center, Institute exp. Pathology, D-6900, Heidelberg, Germany

    M. Trendelenburg, B. Meissner, H. Tröster & H. Spring

  3. Department of Biological Sciences, Dartmouth College, Hanover, NH, USA

    R. D. Allen

  4. Division of Applied Microscopy, Carl Zeiss, D-7082, Oberkochen, Germany

    H. Gundlach

Authors
  1. M. Trendelenburg
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  2. R. D. Allen
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  3. H. Gundlach
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  4. B. Meissner
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  5. H. Tröster
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  6. H. Spring
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Editor information

Editors and Affiliations

  1. Max-Planck-Institut für Biophysik, Kennedyallee 70, 6000, Frankfurt 70, Germany

    Reiner Peters

  2. Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 280, 6900, Heidelberg, Germany

    Michael Trendelenburg

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© 1986 Springer-Verlag, Berlin Heidelberg

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Trendelenburg, M., Allen, R.D., Gundlach, H., Meissner, B., Tröster, H., Spring, H. (1986). Recent Improvements in Microscopy Towards Analysis of Transcriptionally Active Genes and Translocation of RNP-Complexes. In: Peters, R., Trendelenburg, M. (eds) Nucleocytoplasmic Transport. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-71565-5_9

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  • DOI: https://doi.org/10.1007/978-3-642-71565-5_9

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-71567-9

  • Online ISBN: 978-3-642-71565-5

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