Abstract
Fluorescence methods have emerged as indispensable analytical and tracer techniques for a diverse range of biochemical, biophysical and physical studies that depend upon highly sensitive measurements of physical environment. This emergence has been hastened by advances in low-level light detection and by the development of coherent, variable-wavelength, and polarized excitation sources. Moreover, the spectrum of biological and physical applications of fluorescence methods has been expanded broadly by the development and availability of fluorescent molecules with specifically tailored characteristics. The attachment of these fluorescent molecules to molecular species has become a powerful approach for investigating the structure and dynamics of complex systems.
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© 1986 Springer-Verlag, Berlin Heidelberg
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Krafft, G.A. et al. (1986). Fluorescence Photoactivation and Dissipation (FPD). In: Peters, R., Trendelenburg, M. (eds) Nucleocytoplasmic Transport. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-71565-5_4
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DOI: https://doi.org/10.1007/978-3-642-71565-5_4
Publisher Name: Springer, Berlin, Heidelberg
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