Skip to main content

A Pool of Soluble Nuclear Lamins in Eggs and Embryos of Xenopus laevis

  • Conference paper
Nucleocytoplasmic Transport

Abstract

The growing oocyte of the amphibian Xenopus laevis accumulates and stores large pools of various RNAs3 ribonucleoproteins3 and proteins (1–6). These stored molecules are sufficient to meet the needs of the embryo during the first 12 cleavage cycles until RNA synthesis starts at the midblastula stage ca. 4 hours after fertilization (7). Recent experiments indicate that Xenopus eggs also contain stored nuclear envelope components (8, 9). When chromatin or purified DNA are injected into eggs or incubated in extracts of activated eggs nucleus-like structures are assembled possessing nuclear pores and a nuclear lamina.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Similar content being viewed by others

References

  1. Pestell, R.Q.W. (1975) Biochem. J. 145: 527–534.

    PubMed  CAS  Google Scholar 

  2. Laskey, R.A., Mills, A.D. & Morris, N.R. (1977) Cell 10: 237–243.

    Article  PubMed  CAS  Google Scholar 

  3. Woodland, H.R. & Adamson, E.D. (1977) Dev. Biol. 57: 118–135.

    Article  PubMed  CAS  Google Scholar 

  4. DeRobertis, E.M., Lienhard, S. & Parisot, R.F. (1982) Nature 295: 572–577.

    Article  PubMed  Google Scholar 

  5. Kleinschmidt, J.A., Scheer, U., Dabauvalle, M.C., Bustin, M. & Franke, W.W. (1983) J. Cell Biol. 97: 838–848.

    Article  PubMed  CAS  Google Scholar 

  6. Zeller, R., Nyffenegger, T. & DeRobertis, E.M. (1983) Cell 32: 425–434.

    Article  PubMed  CAS  Google Scholar 

  7. Newport, J.W. & Kirschner, M. (1982) Cell 30: 675–686.

    Article  PubMed  CAS  Google Scholar 

  8. Lohka, M.J. & Masui, Y. (1983) Science 220: 719–721.

    Article  PubMed  CAS  Google Scholar 

  9. Forbes, D.J., Kirschner, M. & Newport, J.W. (1983) Cell 34: 13–23.

    Article  PubMed  CAS  Google Scholar 

  10. Krohne, G., Franke, W.W. & Scheer, U. (1978) Exp. Cell Res. 116: 85–102.

    Article  PubMed  CAS  Google Scholar 

  11. Krohne, G., Dabauvalle, M.C. & Franke, W.W. (1981) J. Mol. Biol. 151: 121–141.

    Article  PubMed  CAS  Google Scholar 

  12. Krohne, G., Debus, E., Osborn, M., Weber, K. & Franke, W.W. (1984) Exp. Cell Res. 150: 47–59.

    Article  PubMed  CAS  Google Scholar 

  13. Benavente, R. & Krohne, G. (1985) Proc.Natl.Acad.Sci.USA 82: 6176–6180.

    Article  PubMed  CAS  Google Scholar 

  14. Krohne, G. & Benavente, R. (1986) Exp. Cell Res. 162: 1–10.

    Article  PubMed  CAS  Google Scholar 

  15. Benavente, R., Krohne, G. & Franke, W.W. (1985) Cell 41: 177–190.

    Article  PubMed  CAS  Google Scholar 

  16. Scheer, U., Kartenbeck, J., Trendelenburg, M., Stadler, J. & Franke, W.W. (1976) J. Cell Biol. 69: 1–18.

    Article  PubMed  CAS  Google Scholar 

  17. Aaronson, R.P. & Blobel, G. (1975) Proc.Natl.Acad.Sci.USA 72: 1007–1011.

    Article  PubMed  CAS  Google Scholar 

  18. Benavente, R., Krohne, G., Schmidt-Zachmann, M.S., Hügle, B. & Franke, W.W. (1984) J. Cell Sci., suppl. 1: 161–186.

    CAS  Google Scholar 

  19. Gerace, L. & Blobel, G. (1982) Cold Spring Harbor Symp. Quant. Biol. 46: 967–978.

    PubMed  Google Scholar 

  20. Miake-Lye, R. & Kirschner, M. (1985) Cell 41: 165–175.

    Article  PubMed  CAS  Google Scholar 

  21. Stick, R. & Hausen, P. (1985) Cell 4l: 191–200.

    Article  Google Scholar 

  22. Nieuwcoop, P.D. & Faber, J. (1967) Normal Table of Xenopus laevis (Daudin). North-Holland Publishing Co., Amsterdam.

    Google Scholar 

  23. Gerace, L., Comeau, C. & Benson, M. (1984) J. Cell Sci., suppl 1: 137–160.

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 1986 Springer-Verlag, Berlin Heidelberg

About this paper

Cite this paper

Krohne, G., Benavente, R. (1986). A Pool of Soluble Nuclear Lamins in Eggs and Embryos of Xenopus laevis . In: Peters, R., Trendelenburg, M. (eds) Nucleocytoplasmic Transport. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-71565-5_12

Download citation

  • DOI: https://doi.org/10.1007/978-3-642-71565-5_12

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-71567-9

  • Online ISBN: 978-3-642-71565-5

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics