Abstract
Cellular differentiation is a complex process for which the molecular mechanisms are poorly understood. One useful model for the study of differentiation is the Friend virus derived (Friend 1971) mouse erythroleukemia (MEL) cell lines. These transformed early erythroid precursors can be induced to terminally differentiate into mature erythroid cells by a variety of seemingly unrelated agents including dimethyl, sulfoxide (DMSO) (Marks 1978). Following DMSO induction, the majority of cells become irreversibly committed to differentiation, undergoing several rounds of cell division before ceasing to replicate (Gusella 1976; Fibach 1977). These terminally differentiated cells express high levels of hemoglobin (Boyer 1972; Ostertag 1972). After DMSO induction of MEL cells there is a biphasic decline in steady state levels of c-myc (Lachman 1984; Kirsch 1985) and c-myb mRNAs (Kirsch 1985). We sought to determine if the down regulation of c-myc mRNA is a necessary step for DMSO induced differentiation. Our experiments indicate that expression of a transfected human c-myc gene inhibits the terminal differentiation of MEL cells.
An erratum to this chapter is available at http://dx.doi.org/10.1007/978-94-009-7651-1_55
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Boyer SH, Wuu KD, Noyes AN, Young R, Scher W, Friend C, Preisler HD, Bank A (1972) Hemoglobin biosynthesis in murine virus-induced leukemic cells in vitro: structure and amounts of globin chains produced. Blood 40: 823–835
Chirgwin JM, Przybyla AE, MacDonald RJ, Rutter WJ (1979) Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochem 18: 5294–5299
Davis L, Dibner M, Battey J (1986) In: Methods in molecular biology, Elsevier Publishing Co. (in press)
Fibach E, Reuben RC, Rifkind RA, Marks PA (1977) Effect of hexamethylene bisacetamide on the commitment to differentiation of murine erythroleukemia cells. Cancer Res 37: 440–444
Friend C, Scher W, Holland JG, Sato T (1971) Hemoglobin synthesis in murine virus-induced leukemic cells in vitro: stimulation of erythroid differentiation by dimethyl sulfoxide. Proc Natl Acad Sci USA 68: 378–382
Graham FL, Van der Eb AJ (1973) A new technique for the assay of infectivity of human adenovirus 5 DNA. Virology 52: 456–467
Gusella J, Geller R, Clarke B, Weeks V, Housman D (1976) Commitment to erythroid differentiation by Friend erythroleukemia cells: a stochastic analysis. Cell 9: 221–229
Kirsch IR, Bertness V, Silver J, Hollis G (1985) Oncogene regulation during erythroid differentiation. In: Leukemia: recent advances in biology and treatment, Alan R Liss, Inc. p 91
Lachamn HM, Skoultchi AI (1984) Expression of c-myc changes during erythroid differentiation of mouse erythroleukemia cells. Nature 310: 592–594
Lau Y-F, Kan YW (1983) Versatile cosmid vectors for the isolation, expression, and rescue of gene sequences: studies with the human a-globin gene cluster. Proc Natl Acad Sci USA 80: 5225–5229
Marks PA, Rifkind RA (1978) Erythroleukemic differentiation. Ann Rev Biochem 47: 419–448
Ostertag W, Melderis H, Steinheider G, Kluge N, Dube S (1972) Synthesis of mouse haemoglobin and globin mRNA in leukaemic cell cultures. Nature (London) New Biol 239: 231–234
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© 1986 Springer-Verlag Berlin · Heidelberg
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Dmitrovsky, E., Kuehl, W.M., Hollis, G.F., Kirsch, I.R., Bender, T.P., Segal, S. (1986). A Transfected c-myc Oncogene Inhibits Mouse Erythroleukemic Differentiation. In: Melchers, F., Potter, M. (eds) Mechanisms in B-Cell Neoplasia. Current Topics in Microbiology and Immunology, vol 132. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-71562-4_48
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DOI: https://doi.org/10.1007/978-3-642-71562-4_48
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