Induction of Clonal Monocyte/Macrophage Tumors in vivo by a Mouse c-myc Retrovirus: Evidence for Secondary Transforming Events
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Activation of the c-myc proto-oncogene, in the form of DNA rearrangements that lead to constitutive expression, has been implicated in the genesis of a wide range of tumors. It is therefore of great interest to determine the influence of c-myc oncogene activation on cellular growth control, especially in primary cells. To facilitate the efficient transfer of an activated c-myc oncogene, we have developed a mouse retrovirus that contains the c-myc protein coding sequences and which can be transmitted in the presence of a Mo-MuLV helper virus or established as a helper-free stock using a retroviral packaging cell line. Infection of bone marrow cells gave rise to partially transformed mononuclear phagocytes which were entirely dependent on an exogenous supply of the monocyte specific-growth factor CSF-1 for proliferation. Infection in vivo induced monocyte/macrophage tumors with a latency period of 8–10 wks.
KeywordsConditioned Medium Mononuclear Phagocyte Tumor Line Helper Virus Myeloid Cell Differentiation
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