Induction of Clonal Monocyte/Macrophage Tumors in vivo by a Mouse c-myc Retrovirus: Evidence for Secondary Transforming Events

  • William R. Baumbach
  • E. Richard Stanley
  • Michael D. Cole
Conference paper
Part of the Current Topics in Microbiology and Immunology book series (CT MICROBIOLOGY, volume 132)


Activation of the c-myc proto-oncogene, in the form of DNA rearrangements that lead to constitutive expression, has been implicated in the genesis of a wide range of tumors. It is therefore of great interest to determine the influence of c-myc oncogene activation on cellular growth control, especially in primary cells. To facilitate the efficient transfer of an activated c-myc oncogene, we have developed a mouse retrovirus that contains the c-myc protein coding sequences and which can be transmitted in the presence of a Mo-MuLV helper virus or established as a helper-free stock using a retroviral packaging cell line. Infection of bone marrow cells gave rise to partially transformed mononuclear phagocytes which were entirely dependent on an exogenous supply of the monocyte specific-growth factor CSF-1 for proliferation. Infection in vivo induced monocyte/macrophage tumors with a latency period of 8–10 wks.


Conditioned Medium Mononuclear Phagocyte Tumor Line Helper Virus Myeloid Cell Differentiation 
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Copyright information

© Springer-Verlag Berlin · Heidelberg 1986

Authors and Affiliations

  • William R. Baumbach
    • 1
  • E. Richard Stanley
    • 2
    • 3
  • Michael D. Cole
    • 1
  1. 1.Department of Molecular BiologyPrinceton UniversityPrincetonUSA
  2. 2.Department of Microbiology and ImmunologyAlbert Einstein College of MedicineNew YorkUSA
  3. 3.Department of Cell BiologyAlbert Einstein College of MedicineNew YorkUSA

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