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Studies on Transferrin Receptor Expression in Mouse Plasmacytoma Cells

  • L. M. Neekers
  • R. Nordan
  • S. Bauer
  • M. Potter
Conference paper
Part of the Current Topics in Microbiology and Immunology book series (CT MICROBIOLOGY, volume 132)

Abstract

Transferrin receptor (TfR) expression in lymphoid cells is normally regulated by interaction of tissue-specific growth factors with their receptors (Neckers and Cossman 1983, Neckers et al. 1984). Malignant transformation of T and B lymphocytes generally leads to constitutive expression of TfR (Neckers 1985). Mouse plasmacytomas are an example of terminally differentiated B cells which continue to proliferate in an uncontrolled manner and constitutively express TfR in vivo. However, in vitro growth of most plasmacytomas requires the presence of a macrophage-derived growth factor (plasmacytoma growth factor, PCT-GF) described elsewhere in this volume (Nordan et al.; see also Nordan and Potter 1986). In this report we characterize the relationship between PCT-GF and TfR in plasmacytoma cell lines and describe a TfR mRNA species uniquely found in mouse plasmacytomas.

Keywords

Transferrin Receptor Mouse cDNA Plasma Cell Neoplasm Independent Cell Line Plasmacytoma Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Neckers LM, Cossman J (1983) Transferrin receptor induction in mitogen- stimulated human T lymphocytes is required for DNA synthesis and cell division and is regulated by interleukin 2. Proc Natl. Acad. Sci. USA 80: 3494–3498PubMedCrossRefGoogle Scholar
  2. Neckers LM, Yenokida G, James SP (1984) The role of the transferrin receptor in human B lymphocyte activation. J Immunol 133: 2437–2441PubMedGoogle Scholar
  3. Neckers LM (1985) Transferrin receptor regulation of proliferation in normal and neoplastic B cells. Curr Top Microbiol Immunol 113: 62–68CrossRefGoogle Scholar
  4. Nordan R, Potter M (to be published) A macrophage-derived factor required by plasmacytoma cells for survival and proliferation in vitro. ScienceGoogle Scholar
  5. McClelland A, Kuhn LC, Ruddle FH (1984) The human transferrin receptor gene: Genomic organization, and the complete primary structure of the receptor deduced from a cDNA sequence. Cell 39: 267–274Google Scholar
  6. Kuhn LC, McClelland A, Ruddle FH (1984) Gene transfer, expression, and molecular cloning of the human transferrin receptor gene. Cell 37: 95–103PubMedCrossRefGoogle Scholar
  7. Anderson PN and Potter M (1969) Induction of plasma cell tumors in BALB/c mice with 2,6,10,14-tetramethylpentadecane (pristane). Nature 222: 994–995PubMedCrossRefGoogle Scholar
  8. Potter M and Boyce C (1962) Induction of plasma cell neoplasms in strain BALB/c mice with mineral oil and mineral oil adjuvants. Nature 193: 1086–1087PubMedCrossRefGoogle Scholar
  9. Potter M, Sklar MD and Rowe WP (1973) Rapid viral induction of plasmacytomas in pristane primed BALB/c mice. Science 182: 592–594PubMedCrossRefGoogle Scholar
  10. Kuhn LC (1985) Human transferrin receptor expressed from a transfected cDNA is functional in murine cells. In: Spik G, Montreuil J, Crichton RR, and Mazurier J. (eds.) Proteins of Iron Storage and Transport, Elsevier Science Publishers, New York, p. 369Google Scholar
  11. Rapp UR, Cleveland JL, Fredrickson TN, Holmes KL, Morse III HC, Jansen HW, Patschinsky T and Bister K (1985) Rapid induction of hemopoietic neoplasms in newborn mice by a raf(mil)/myc recombinant murine retrovirus. J Virol 55: 23–33PubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin · Heidelberg 1986

Authors and Affiliations

  • L. M. Neekers
    • 1
    • 2
  • R. Nordan
    • 1
    • 2
  • S. Bauer
    • 1
    • 2
  • M. Potter
    • 1
    • 2
  1. 1.Laboratory of PathologyNCI, NIHBethesdaUSA
  2. 2.Laboratory of GeneticsNCI, NIHBethesdaUSA

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