Abstract
In BALB/c mice, plasmacytomas (PCT) arise and proliferate in the granulomatous tissue that forms in response to the intraperitoneal administration of pristane (Potter and Boyce, 1962; Anderson and Potter, 1969). Studies by Cancro and Potter (1976) suggest that the oil granuloma provides a special microenvironment which is necessary for the growth of early PCT’s. Although the specific contributions of this microenvironment are unknown, in vitro studies suggest that PCT’s that have not been adapted previously to cell culture require added factors for in vitro growth and proliferation. Namba and Hanaoka (1972) described a 50 kDa macrophage-derived protein that was required for the in vitro growth of the M0PC104E PCT. Metcalf (1974) demonstrated that mouse serum or peritoneal macrophages supported the clonal growth of PCT’s in soft agar. Recently, Corbel and Mel chers (1984) reported that “alpha factor-containing” supernatant from the P388D1 cell line also supported the in vitro growth of several PCT’s. We have recently reported the establishment of PCT cell lines that are totally dependent on a factor for survival and proliferation in vitro (Nordan and Potter, 1986). We discuss here our initial biological and biochemical characterizations of this factor.
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References
Anderson PN, Potter M (1969) Induction of plasma-cell tumours in BALB/c mice with 2,6,10,14-tetramethylpentadecane (pristane). Nature (London) 222: 994–995
Cancro M, Potter M (1976) The requirement of an adherent substratum for the growth of developing plasmacytoma cells in vivo. J Exp Med 144: 1554–1566
Corbel C, Melchers F (1984) The synergism of assessory cells and of soluble a-factors derived from them in the activation of B cells to proliferation. Immun Rev 78: 51–74
Lachman LB, Hacker MP, Blyden GT, Handschumacher RE (1977) Preparation of lymphocyte-activating factor from continuous murine macrophage cell lines. Cell Immunol 34: 416–423
Metcalf D (1974) The serum factor stimulating colony formation in vitro by murine plasmacytoma cells: response to antigens and mineral oil. J Immunol 113: 235–243
Mizel SB, Oppenheim JJ, Rosenstreich DL (1978) Characterization of lymphocyte-activating factor (LAF) produced by the macrophage cell line, P388D1. I. Enhancement of LAF production by activated T lymphocytes. J Immunol 120: 1497–1503
Namba Y, Hanaoka M (1972) Immunocytology of cultured IgM-forming cells of mouse. I. Requirement of phagocytic cell factor for the growth of IgM-forming tumor cells in tissue culture. J Immunol 109: 1193–1200
Nordan RP, Potter M (1986) A macrophage-derived factor required by plasmacytomas for survival and proliferation in vitro. Science (in press)
Potter M (1962) Induction of plasma-cell neoplasms in strain BALB/c mice with mineral oil and mineral oil adjuvants. Nature (London) 193: 1086–1087
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© 1986 Springer-Verlag Berlin · Heidelberg
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Nordan, R.P., Neckers, L.M., Rudikoff, S., Potter, M. (1986). A Growth Factor Required by Plasmacytoma Cells In Vitro . In: Melchers, F., Potter, M. (eds) Mechanisms in B-Cell Neoplasia. Current Topics in Microbiology and Immunology, vol 132. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-71562-4_15
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DOI: https://doi.org/10.1007/978-3-642-71562-4_15
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