A Growth-Factor Dependent B-Cell Hybridoma

  • P. M. Lansdorp
  • L. A. Aarden
  • J. Calafat
  • W. P. Zeiljemaker
Conference paper
Part of the Current Topics in Microbiology and Immunology book series (CT MICROBIOLOGY, volume 132)


Injection of pristane (2,6,10,14 tetramethylpentadecane) into the peritoneal cavity of Balb/c mice has been used to induce plasmacytomas (1) and to induce a microenvironment that will support the growth of transplanted plasmacytomas derived from primary hosts (2) and B-cell hybridomas (3). In vitro growth of primary plasmacytomas (4) and B-cell hybrids immediately after fusion (5) requires the addition of “feeder” cells to the culture medium. In previous studies we have shown that this feeder-cell requirement of B-cell hybrids can be overcome by the addition of the supernatant of human endothelial cell cultures to the culture medium (6,7). The characterization of this growth-promoting activity has been hampered by the lack of rapid and reproducible assay.


Conditioned Medium Rough Endoplasmatic Reticulum Human Peripheral Blood Leukocyte Reproducible Assay Fetal Skin Fibroblast 
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  1. 1.
    Anderson, PN and M Potter (1969) Nature 222: 994PubMedCrossRefGoogle Scholar
  2. 2.
    Potter, M, Pumphrey, JG and JL Walters (1972) J Natl Cancer Inst 49: 305PubMedGoogle Scholar
  3. 3.
    Brodeur, BR, Tsang, P and Y Larose (1984) J Immunol Methods 71: 265PubMedCrossRefGoogle Scholar
  4. 4.
    Potter M and M Cancro (1978) in: Cell Differentiation and Neoplasia. GF Saunders ( Ed) Raven Press, New York, pp 145–161Google Scholar
  5. 5.
    Fazekas de St Groth, S and D Scheidegger (1980) J Immunol Methods 35: 1Google Scholar
  6. 6.
    Astaldi, GCB, Janssen MC, Lansdorp PM, Willems, C, Zeijlemaker and F Oosterhof (1980) J Immunol 125: 1411PubMedGoogle Scholar
  7. 7.
    Astaldi GCB, Janssen MC, Lansdorp PM, Zeijlemaker WP and C Willems (1981) J Immunol 126: 1170PubMedGoogle Scholar
  8. 8.
    Aarden LA, Lansdorp PM and E de Groot (1985) in: Lymphokines, vol 10, Academic press, pp 175–185Google Scholar
  9. 9.
    Shulman M and G Kohler (1978) Nature 276: 269PubMedCrossRefGoogle Scholar
  10. 10.
    Tetteroo PAT, Lansdorp PM, Geurts van Kessel, AH, Hagemeijer A and AEGKr von dem Borne (1984) in: Human Leucocyte Differentiation Antigens, H Gotze (Ed) Springer-Verlag Heidelberg, pp 419–423Google Scholar
  11. 11.
    Lansdorp, PM, Astaldi GCB, Oosterhof, F, Janssen MC, and WP Zeijlemaker (1980) J Immunol Meth 39: 393CrossRefGoogle Scholar
  12. 12.
    Uytendaal MP, de Bruyn CHMM, Hatanaka M and P Hosli (1979) In Vitro 15: 103CrossRefGoogle Scholar
  13. 13.
    Worton and Duff (1979) in: Methods in Enzymology, vol 58 Jacoby WB and IH Pastan ( Eds) Academic Press, New York, pp 322–344Google Scholar
  14. 14.
    Ross, R (1971) J Cell Biol 50: 172PubMedCrossRefGoogle Scholar
  15. 15.
    De Groot, PhG, Strijland A, Kaisbeek, R, Meera Khan P, Westerveld A, Hamers MN and JM Tager (1980) Exp Cell Res 126: 207Google Scholar
  16. 16.
    Schlunk T and M Schleyer (1980) Exp Hematol 8: 179PubMedGoogle Scholar
  17. 17.
    Du Bois, MJGJ, Meinesz, A, Bierhorst, A, Groenewoud, M, Schellekens PThA and VP Eijsvoogel (1974) Tissue Antigens 4: 458CrossRefGoogle Scholar
  18. 18.
    Schultz FW, Mulder JA and P Sonneveld (1986) Anal Quant Cytometry (in press)Google Scholar
  19. 19.
    Namba, Y and M Hanaoka (1972) J Immunol 109: 1193PubMedGoogle Scholar
  20. 20.
    Metcalf D (1974) J Immunol 113: 235PubMedGoogle Scholar
  21. 21.
    Moller G (Ed) (1984) Immunol Rev 78, Munksgaard CopenhagenGoogle Scholar
  22. 22.
    Weiss RA (1982) Immunol Today 3: 292CrossRefGoogle Scholar
  23. 23.
    Rudolph, M, Karsten, U and M Micheel (1983) Immunol Today 4: 185CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin · Heidelberg 1986

Authors and Affiliations

  • P. M. Lansdorp
    • 1
  • L. A. Aarden
    • 1
  • J. Calafat
    • 2
  • W. P. Zeiljemaker
    • 1
  1. 1.Central Laboratory of the Netherlands Red Cross Blood Transfusion Service and Laboratory for Experimental and Clinical ImmunologyUniversity of AmsterdamAmsterdamThe Netherlands
  2. 2.Netherlands Cancer InstituteAmsterdamThe Netherlands

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