An In Vitro Model for Tumor Progression in Murine Lymphoid Cells

  • Richard C. Schwartz
  • Lawrence W. Stanton
  • Kenneth B. Marcu
  • Owen N. Witte
Conference paper
Part of the Current Topics in Microbiology and Immunology book series (CT MICROBIOLOGY, volume 132)


Histopathological observations of the development of tumors have led to the concept that oncogenesis proceeds through the sequential acquisition of independent growth-related phenotypic characteristics (Foulds 1975). This process of tumor progression occurs through events involving the expression of specific genes affecting the regulation of cellular growth (recently reviewed in Weinberg 1985; Klein and Klein 1985; Bishop 1985). The requirement of two complementing oncogenes for the in vitro transformation of primary rat embryo firbroblasts provides a useful in vitro experimental model of tumor progression. Ha-ras (EJ) and v-myc or Ha-ras (T24) and E1A can act together to transform primary fibroblasts, while neither oncogene acting alone is capable of causing efficient transformation (Land et al. 1983; Ruley 1983) unless expressed at a high level (Spandidos and Wilkie 1984).


Slot Blot Cell Neoplasia Soft Agar Medium Adherent Bone Marrow Murine Plasmacytoma 
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Copyright information

© Springer-Verlag Berlin · Heidelberg 1986

Authors and Affiliations

  • Richard C. Schwartz
    • 1
  • Lawrence W. Stanton
    • 2
  • Kenneth B. Marcu
    • 2
  • Owen N. Witte
    • 1
  1. 1.Department of Microbiology and Molecular Biology InstituteUniversity of CaliforniaLos AngelesUSA
  2. 2.Biochemistry DepartmentState University of New YorkStony BrookUSA

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