Abstract
The reconstitution of coupled electron transport in cytochrome c oxidase is realized by removal of cholate from a starting suspension of purified complex (prepared as described by Errede et al. 1978) and a mixture of phospholipids. The reconstituted system so obtained (proteoliposomes) is constituted by small unilamellar vesicles with a diameter of 500–600 Å (Casey et al. 1979, Racker 1979, Nicholls 1981).
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References
Brunori M, Colosimo A, Rainoni O, Wilson MT, Antonini E (1979) J Biol Chem 254: 10769–10775
Casey RP, Chappel JB, Azzi A (1979) Biochem J 182: 149–156
Errede B, Kamen MD, Hatefi Y (1978) Methods Enzymol 55: 614–627
Michell P, Moyle J (1983) FEBS Lett 151: 167–178
Nicholls P (1981) Int Rev Cytol Suppl 12: 327–388
Racker E (1979) Methods Enzymol 55: 699–711
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© 1986 Springer-Verlag Berlin Heidelberg
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Capitanio, N., Papa, S. (1986). Reconstitution of Cytochrome c Oxidase. In: Azzi, A., Masotti, L., Vecli, A. (eds) Membrane Proteins. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-71543-3_18
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DOI: https://doi.org/10.1007/978-3-642-71543-3_18
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-17014-3
Online ISBN: 978-3-642-71543-3
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