Abstract
Microsequencing of polypeptides by the Edman degradation technique can be performed either manually or by automated methods [1, 2]. The degradation consists of three parts, the coupling with phenylisothiocyanate (PITC) or an appropriate homolog; the acid cleavage of the first amino acid as phenylthiazolinone from the peptide chain, and the isomerization to the more stable phenylthiohydantoins. This then has to be identified, e.g., by thin-layer techniques [2] or high performance liquid chromatography (HPLC) [3].
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© 1986 Springer-Verlag Berlin Heidelberg
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Wittmann-Liebold, B., Hirano, H., Kimura, M. (1986). Manual Microsequence Determination of Proteins and Peptides with the DABITC/PITC Method. In: Wittmann-Liebold, B., Salnikow, J., Erdmann, V.A. (eds) Advanced Methods in Protein Microsequence Analysis. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-71534-1_8
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DOI: https://doi.org/10.1007/978-3-642-71534-1_8
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