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Water Contents and Quality Criteria of Microsequencing Chemicals. Preliminary Results of a Reevaluation

  • Lothar Meinecke
  • Harald Tschesche

Abstract

The sequential degradation of peptides and proteins by the manual technique of Edman [1] and the automated procedure of Edman and Begg [2] requires several reagents and solvents of high quality. The general principle of this technique and the kind of chemicals needed have scarcely been changed since then. This is rather surprising, because since its introduction the protein quantity to be sequenced has decreased from 300–1000 nmol to less than 200 pmol of material that allow determination of up to 40 amino acid residues of an unknown sequence.

Keywords

Molecular Sieve Purification Procedure Sequanal Grade Reflux Ratio Glass Ring 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1986

Authors and Affiliations

  • Lothar Meinecke
    • 1
  • Harald Tschesche
    • 1
  1. 1.Lehrstuhl für BiochemieUniversität Bielefeld, Fakultät für ChemieBielefeldGermany

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