Cytoprotective Effect of Iloprost Against Liver Cell Death Induced by Carbon Tetrachloride (CCl₄) or Bromobenzene

Abstract

In the last decade a series of eicosanoids have been found to protect mammalian cells against various forms of acute cell damage [4, 5]. Among these eicosanoids, the prostacyclin derivative Iloprost (ZK 36374) exerts cytoprotective activity in myocardial ischemia and gastrointestinal ulceration [6]. The aim of the present study was to investigate whether or not Iloprost (ZK 36 374) and the stereoisomer (ZK 36375) would also exhibit cytoprotective action on rat hepatocytes. Testing of Iloprost for cytoprotective potency against acute hepatocellular injury was performed with primary cultures of rat hepatocytes as well as with intact rats. Carbon tetrachloride and bromobenzene were used as model toxins to induce severe liver cell damage. Cell damage was assessed by examining hepatocellular morphology using light microscopy, and also by examining vital dye exclusion, release of cytoplasmic enzymes into culture medium or blood, respectively, and mortality of animals.