EXAFS and XANES Studies of High-Valent Metal-Oxo Porphyrins Relevant to Horseradish Peroxidase

  • J. E. Penner-Hahn
  • K. Smith Eble
  • J. H. Dawson
  • T. J. McMurry
  • J. T. Groves
  • M. Benfatto
  • S. Doniach
  • B. Hedman
  • K. O. Hodgson
Conference paper
Part of the Springer Series in Biophysics book series (BIOPHYSICS, volume 2)

Abstract

Horseradish peroxidase is a heme-containing enzyme that catalyzes the oxidation of phenolic substrates, utilizing hydrogen peroxide as the ultimate electron acceptor. The resting form of HRP contains a high-spin ferric heme. During the catalytic cycle, HRP is initially oxidized by two electrons to a green species known as Compound I (HRP-I). One electron reduction of HRP-I yields the red species known as Compound II (HRP-II), that retains one oxidizing equivalent above the resting enzyme. There is substantial interest in understanding the chemistry of high-valent transition metal species in general and the peroxidases in particular because of their importance in oxidation and oxygenation reactions. In this report we review our recent EXAFS and XANES studies of high-valent metalloporphyrins which are relevant to horseradish peroxidase compounds I and II.

Keywords

Peroxide Ethyl Hydroxyl Porphyrin Hydroperoxide 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1987

Authors and Affiliations

  • J. E. Penner-Hahn
    • 1
  • K. Smith Eble
    • 2
  • J. H. Dawson
    • 2
  • T. J. McMurry
    • 3
  • J. T. Groves
    • 3
  • M. Benfatto
    • 4
  • S. Doniach
    • 4
  • B. Hedman
    • 5
  • K. O. Hodgson
    • 5
  1. 1.Department of ChemistryMichigan UniversityAnn ArborUSA
  2. 2.Department of ChemistrySouth Carolina UniversityColumbiaUSA
  3. 3.Department of ChemistryPrinceton UniversityPrincetonUSA
  4. 4.Department of Applied PhysicsStanford UniversityStanfordUSA
  5. 5.Department of ChemistryStanford UniversityStanfordUSA

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