Advertisement

Identification of a cDNA Transcript Containing the Coding Region for F-I Globin from Urechis caupo

  • James R. Carey
  • Austen F. Riggs
Conference paper

Abstract

A cDNA transcript of 729 bp in length coding for Urechis caupo F-I globin has been cloned. The coding region has been completely sequenced. Northern and Southern blot analysis indicate that there may be several genes coding for mRNA transcripts of nearly identical size. This could account for the heterogeneity observed in the hemoglobin.

Keywords

Bohr Effect eDNA Library eDNA Transcript Sanger Dideoxy Method 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Garey, J.R. and Riggs, A.F., Arch. Biochem. Biophys. 228, 320–331(1984).Google Scholar
  2. 2.
    Garey, J.R., Brodeur, R.D., and Riggs, A.F., Life Chem. Reports. Suppl. 1, 387–391 (1983).Google Scholar
  3. 3.
    Cox, R.H., Methods in Enzymology 12B, 120–129 (1968).CrossRefGoogle Scholar
  4. 4.
    Messing, J., Methods in Enzymology 101, 20–78 (1983).PubMedCrossRefGoogle Scholar
  5. 5.
    Huynh, T.V., Young, R.A., and Davis, R.W., in DNA Cloning: A Practical Approach (Glover, D., ed.) IRL Press, Oxford, pp. 49–78 (1985).Google Scholar
  6. 6.
    Maniatis, T., Fritsch, E.F. and Sambrook, J., Molecular Cloning, a laboratory manual. Cold Spring Harbor Laboratory. (1982).Google Scholar
  7. 7.
    Sanger, F., Nicklen, S., and Coulson, A.R., Proc. Natl.Acad. Sci. (USA) fio, 5463–5487 (1977).Google Scholar
  8. 8.
    Blin, N. and Stafford, D.W., Nuc. Acid Rea. 3, 2303–2308 (1976).CrossRefGoogle Scholar
  9. 9.
    Fisher, W.K., Gilbert, A.T., and Thompson, E.O.P., Aust. J. Biol. Sci. 37, 191–203 (1980).Google Scholar
  10. 10.
    Como, P.F., and Thompson, E.O.P., Aust. J. Biol. Sci. 33, 653–664 (1980).Google Scholar
  11. 11.
    Garlick, R.L. and Riggs, A.F., J. Biol. Chem. 26T, 9005–9015 (1982).Google Scholar
  12. 12.
    Imamura, T., Baldwin, T.O., and Riggs, A., J. Biol. Chem. 247, 2785–2797 (1972).PubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1986

Authors and Affiliations

  • James R. Carey
    • 1
  • Austen F. Riggs
    • 1
  1. 1.Department of ZoologyUniversity of TexasAustinUSA

Personalised recommendations