The Reaction of Hemocyanin from Octopus Vulgaris with Nitrite. A Reinterpretation of the Oxidation Pathways

  • B. Salvato
  • G. M. Giacometti
  • M. Alviggi
  • G. Giacometti
Conference paper


The reaction of Hemocyanin with nitric oxide has been observed since a long time (1). An interpretation in terms of redox reaction was first proposed by Schoot Uiterkamp (2) and Schoot Uiterkamp and Mason (3). Different chemical schemes have been reported since then to explain this reaction. In all cases NO was assumed to be the oxidant, whereas different interpretation were advanced about the nature and state of ligation of the oxidation product (4,5,6). According to a recent review by Solomon (7), the generally accepted chemistry of oxidation of molluscan hemocyanin involves the oxidation of the deoxy form by NO to an half-met-NO 2 intermediate which can be further oxidized to a “dimer” (i.e. an EPR detectable met) in the form of a [Cu(II) CU(II)]NxOy derivative.


Nitric Oxide Oxygen Carrier Sulfamic Acid Copper Oxidation Chemical Scheme 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. (1).
    C. Dhéré, J. Physiol. Pathol. Gen., 18, 503 (1919).Google Scholar
  2. (2).
    Schoot Uiterkamp, A.J.M., FEBS Lett. 20, 93 (1972).CrossRefGoogle Scholar
  3. (3).
    Schoot Uiterkamp, A.J.M. and Mason, H.S., Proc. Nat. Acad. Sci. U.S.A., 70 993 (1973).CrossRefGoogle Scholar
  4. (4).
    Van der Deen, H. and Hoving, H., Biochemistry 16, 3519 (1977).PubMedCrossRefGoogle Scholar
  5. (5).
    Himmelwright, R.S., Eickman, N.C., and Solomon, E.I., Biochem. Biophys. Res. Commun., 81, 237 (1978).CrossRefGoogle Scholar
  6. (6).
    Verplaetze, J., Van Tornout P., Defrejn G., Witters R., and Lontie R., Eur. J. Biochem. 95, 327 (1979).CrossRefGoogle Scholar
  7. (7).
    Solomon E.I., in “Copper Proteins”red. T.S. Spiro, J. Wiley and Sons, New York, pag. 43 (1981).Google Scholar
  8. (8).
    Salvato B., Ghiretti Magaldi A. and Ghiretti F., Biochemistry 13, 4778 (1974).PubMedCrossRefGoogle Scholar
  9. (9).
    Vogel A.I. “A textbook of Quantitative Inorganic Analysis”.Lowe and Brydone, London (1961).Google Scholar
  10. (10).
    Cotton F.A. and Wilkinson G., “Advanced Inorganic Chemistry Interscience (1972).Google Scholar
  11. (11).
    Dunford H.B. and Nadezhdin A.D. in “Oxygen and Oxy-Radicals in Chemistry and Biology”, Rodgers M.A.J. and Power E.L.,eds., pag. 625, Acad.Press. (1981).Google Scholar
  12. (12).
    Salvato B., Joni G., Piazzesi A., Ghiretti F., Beltramini M. and Lerch K., Life Chem. Rep., Supp. 1, 313 (1983).Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1986

Authors and Affiliations

  • B. Salvato
    • 1
  • G. M. Giacometti
    • 1
  • M. Alviggi
    • 1
  • G. Giacometti
    • 1
  1. 1.Department of Biology and Department of Physical ChemistryUniversity of PaduaItaly

Personalised recommendations