The Reaction of Hemocyanin from Octopus Vulgaris with Nitrite. A Reinterpretation of the Oxidation Pathways
The reaction of Hemocyanin with nitric oxide has been observed since a long time (1). An interpretation in terms of redox reaction was first proposed by Schoot Uiterkamp (2) and Schoot Uiterkamp and Mason (3). Different chemical schemes have been reported since then to explain this reaction. In all cases NO was assumed to be the oxidant, whereas different interpretation were advanced about the nature and state of ligation of the oxidation product (4,5,6). According to a recent review by Solomon (7), the generally accepted chemistry of oxidation of molluscan hemocyanin involves the oxidation of the deoxy form by NO to an half-met-NO 2 − intermediate which can be further oxidized to a “dimer” (i.e. an EPR detectable met) in the form of a [Cu(II) CU(II)]NxOy derivative.
KeywordsNitric Oxide Oxygen Carrier Sulfamic Acid Copper Oxidation Chemical Scheme
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- (1).C. Dhéré, J. Physiol. Pathol. Gen., 18, 503 (1919).Google Scholar
- (7).Solomon E.I., in “Copper Proteins”red. T.S. Spiro, J. Wiley and Sons, New York, pag. 43 (1981).Google Scholar
- (9).Vogel A.I. “A textbook of Quantitative Inorganic Analysis”.Lowe and Brydone, London (1961).Google Scholar
- (10).Cotton F.A. and Wilkinson G., “Advanced Inorganic Chemistry Interscience (1972).Google Scholar
- (11).Dunford H.B. and Nadezhdin A.D. in “Oxygen and Oxy-Radicals in Chemistry and Biology”, Rodgers M.A.J. and Power E.L.,eds., pag. 625, Acad.Press. (1981).Google Scholar
- (12).Salvato B., Joni G., Piazzesi A., Ghiretti F., Beltramini M. and Lerch K., Life Chem. Rep., Supp. 1, 313 (1983).Google Scholar