Preparation of a Methaemocyanin of Astacus Leptodactylus Regenerable with Hydroxylamine
The classical preparation of arthropodan methaemocyanin is based on the oxidation of deoxyhaemocyanin with a small excess of hydrogen peroxide (1). The methaemocyanin of the crustacean Astacus leptodactylus and of the chelicerate Limulus polyphemus could not be regenerated with reducing agents like hydroxylamine, hydrazine, nor with hydrogen peroxide. Only the latter could be regenerated markedly with hydrogen sulphide or with hydrogen cyanide (2).
KeywordsPeroxide Microwave Sulphide Nitrite Cyanide
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