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Preparation of a Methaemocyanin of Astacus Leptodactylus Regenerable with Hydroxylamine

  • R. Witters
  • D. Van Hoof
  • W. Deleersnijder
  • J.-P. Tahon
  • R. Lontie
Conference paper

Abstract

The classical preparation of arthropodan methaemocyanin is based on the oxidation of deoxyhaemocyanin with a small excess of hydrogen peroxide (1). The methaemocyanin of the crustacean Astacus leptodactylus and of the chelicerate Limulus polyphemus could not be regenerated with reducing agents like hydroxylamine, hydrazine, nor with hydrogen peroxide. Only the latter could be regenerated markedly with hydrogen sulphide or with hydrogen cyanide (2).

Keywords

Circular Dichroic Spectrum Copper Atom Hydrogen Sulphide Hydrogen Cyanide Nitrogen Monoxide 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

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    Lijnen, H.R., Witters, R., and Lontie, R., Comp. Biochem. Physiol. 63B, 35–38 (1979).Google Scholar
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    Gaykema, W.P.J., Hol, W.G.J., Vereijken, J.M., Soeter, N.M., Bak, H.J., and Beintema, J.J., Nature 309, 23–29 (1984).CrossRefGoogle Scholar
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    Verplaetse, J., Van Tornout, Ph., Defreyn, Gh., Witters, R., and Lontie, R., Eur. J. Biochem. 95, 327–331 (1979).PubMedCrossRefGoogle Scholar
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    Witters, R., Deleersnijder, W., Tahon, J.-P., and Lontie, R., this volume, pp. 449–452 (1986).Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1986

Authors and Affiliations

  • R. Witters
    • 1
  • D. Van Hoof
    • 1
  • W. Deleersnijder
    • 1
  • J.-P. Tahon
    • 1
  • R. Lontie
    • 1
  1. 1.Laboratorium voor BiochemieKatholieke Universiteit to LeuvenLeuvenBelgium

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