Kinetics and Equilibria of Octopus Hemocyanin Association
Molluscan hemocyanins are constructed from decamers of large polypeptide chains (see van Holde and Miller, 1982, Ellerton et al., 1983). Although complete dissociation of these molecules is readily effected, quantitative reassociation has not been possible in most cases where it has been tried (see, for example, van Holde and Cohen, 1964; Siezen, 1974; Brouwer et al., 1978). However, as shown by Miller and van Holde (1982), dissociation of the hemocyanin of Octopus dofleini is completely reversible. Addition of sufficient quantities of a divalent cation (Ca2+, Mg2+) results in the quantitative reassociation of subunits into functional decameric molecules, indistinguishable from the native hemocyanin by physical techniques (van Holde and Miller, 1985). Because of this behavior, we felt that this system would be an excellent candidate for studies of the kinetics of reassociation of a molluscan hemocyanin. Virtually nothing is known of such processes.
KeywordsDivalent Cation Light Scattering Study Entire Time Span Sedimentation Velocity Experiment Light Scatter Experiment
Unable to display preview. Download preview PDF.
- Brouwer, M., Ryan, M., Bonaventura, J. and Bonaventura, C. (1978) Biochemistry 17, 2810–2815.Google Scholar
- Ellerton, H.D., Ellerton, N.F., and Robinson, H. (1983) Prog. Biophys. Mol. Biol. 41, 143–248.Google Scholar
- Miller, K.I., and van Holde, K.E. (1982) Comp. Biochem. Physiol. 73B, 1013–1018.Google Scholar
- Siezen, R.J. (1974) J. Molec. Biol. 90, 103–113.Google Scholar
- van Holde, K.E. and Cohen, L.B. (1974) Brookhaven Symp. Biol. 17, 184–193.Google Scholar
- van Holde, K.E. and Miller, K.I. (1982) Quart. Rev. Biophys. 15, 1–129.Google Scholar
- van Holde, K.E. and Miller, K.I. (1985) Biochemistry (In Press).Google Scholar