Abstract

Proteinaceous “Sperm Receptors” on the egg plasma membrane facilitate binding of sperm (Wolf et al., 1976). The inability of trypsin inhibitors to prevent binding of mouse sperm to eggs (Saling, 1981) suggests that acrosin activity is not required for gamete contact, but Ca2+ is necessary for sperm-egg contact in both hamsters (Yanagimachi, 1982) and mice (Saling, 1981).

Keywords

Sugar Immobilization Trypsin Choline Tated 

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References

  1. Ahuja KK (1985) Inhibitors of glycoprotein biosynthesis block fertilization in the hamster. Gamete Research 11: 179–189CrossRefGoogle Scholar
  2. Aitken RJ, Ross A and Lees ML (1983) Analysis of sperm function in Kartagener’s Syndrome. Fert Steril 40: 696–698Google Scholar
  3. Austin CR (1951) Observations on the penetration of the sperm into the mammalian egg. Aust J Biol Sci B4: 589–596Google Scholar
  4. Austin CR and Bishop MWH (1958) Role of the rodent acrosome and perfatorium in fertilization. Proc Roy Soc (Lond) 149B: 241–248CrossRefGoogle Scholar
  5. Barbanti-Brodano G, Possati L and Laplaca M (1971) Inactivation of polykaryocytogcnic and hemolytic activities of sendai virus by phospholipase B (lysolecithinase). J Virol 8: 796–800PubMedGoogle Scholar
  6. Barros C and Herrera E (1977) Ultrastructural observations of the incorporation of guinea-pig spermatozoa into zona-free hamster oocytes. J Reprod Fert 49: 47–50CrossRefGoogle Scholar
  7. Bedford JM (1967) Experimental requirement for capacitation and observations on ultra-structural changes in rabbit spermatozoa during fertilization. J Reprod Fert Suppl 2: 35–48Google Scholar
  8. Bedford JM (1968) Ultrastructural changes in the sperm head during fertilization in the rabbit. Am J Anat 123: 329–358PubMedCrossRefGoogle Scholar
  9. Bedford JM, Moore HDM and Franklin LE (1979) Significance of the equatorial segment of the acrosome of the spermatozoon in Eutherian mammals. Exp Cell Res 119: 119–126PubMedCrossRefGoogle Scholar
  10. Bendich A, Borenfreund E and Sternberg SS (1974) Penetration of somatic mammalian cells by sperm. Science 183: 857–859PubMedCrossRefGoogle Scholar
  11. Brackett BG, Baranska W, Sawicki W and Koprowski H (1971) Uptake of heterologous genome by mammalian spermatozoa through its transfer to ova through fertilization. Proc Nat Acad Sci US 68: 353–357CrossRefGoogle Scholar
  12. Croce CM, Gledhill BL, Garbara B, Sawicki W and Koprowski H (1972) Lysolecithin-induced fusion of rabbit spermatozoa with hamster somatic cells. Adv Biosci 8: 187–200Google Scholar
  13. Cummins JM and Teichmann RJ (1974) The accumulation of malachite green stainable phospholipid in rabbit spermatozoa during maturation in the epididymis, and its possible role in capacitation. Biol Reprod 10: 555–564PubMedCrossRefGoogle Scholar
  14. Dravland JE and Meizel S (1982) The effect of inhibitors of trypsin and phospholipase A2 on the penetration of zona pellucida-free hamster eggs by acrosome reacted hamster sperm. J Androl 3: 388–395Google Scholar
  15. Elsevier SM and Ruddle FH (1976) Haploid genome reactivation and recovery by cell hybridization. Induction of DNA synthesis in spermatid nuclei. Chromosoma 56: 227–241PubMedCrossRefGoogle Scholar
  16. Friend DS, Orci L, Perrelet A and Yanagimachi R (1977) Membrane particle changes attending the acrosome reaction in guinea pig spermatozoa. J Cell Biol 74: 561–577PubMedCrossRefGoogle Scholar
  17. Gabel CA, Eddy EM and Shapiro BM (1979) After fertilisation, sperm surface components remain as a patch in sea urchin and mouse embryos. Cell 18: 207–215PubMedCrossRefGoogle Scholar
  18. Garbara B, Gledhill BL, Croce CM, Cesarini JP and Koprowski H (1973) Ultrastructure of rabbit spermatozoa after treatment with lysolecithin and in the presence of hamster somatic cells. Proc Soc exp Biol Med 143: 1120–1124Google Scholar
  19. Gaunt SJ (1983) Spreading of a sperm surface antigen within the plasma membrane of the egg after fertilization in the rat. J Embryol exp Morphol 75: 259–270PubMedGoogle Scholar
  20. Gledhill BL, Sawicki W, Croce CM and Koprowski H (1972) DNA synthesis in rabbit spermatozoa after treatment with lysolecithin and fusion with somatic cells. Exp Cell Res 73: 33–40PubMedCrossRefGoogle Scholar
  21. Goeringer GC and Dilliplane D (1976) Some aspects of the fusion of avian erythrocytes and bovine spermatozoa in the presence of chemical fusing agents. Anat Rec 184: 412Google Scholar
  22. Hanada A and Chang MC (1972) Penetration of zona-free eggs by spermatozoa of different species. Biol Reprod 6: 300–309PubMedGoogle Scholar
  23. Hanada A and Chang MC (1978) Penetration of the zona-free or intact eggs by foreign spermatozoa and the fertilization of deer mouse eggs in vitro. J exp Zool 203: 277–286PubMedCrossRefGoogle Scholar
  24. Hartmann JF and Gwatkin RBL (1971) Alteration of sites on the mammalian sperm surface following capacitation. Nature (Lond) 234: 479–481CrossRefGoogle Scholar
  25. Higgins PJ, Borenfreund E and Bendich A (1975) Appearance of foetal antigens in somatic cells after interaction with heterologous sperm. Nature (Lond) 257: 488–489CrossRefGoogle Scholar
  26. Hirao Y and Yanagimachi R (1978a) Effects of various enzymes on the ability of hamster egg plasma membranes to fuse with spermatozoa. Gamete Res 1: 3–12CrossRefGoogle Scholar
  27. Hirao Y and Yanagimachi R (1978b) Temperature dependence of sperm-egg fusion and post-fusion events in hamster fertilization. J exp Zool 205: 433–438PubMedCrossRefGoogle Scholar
  28. Holt WV and Dott HM (1980) Chemically induccd fusion between ram spermatozoa and avian erythrocytes: an ultrastructural study. J Ultrastruct Res 71: 311–320PubMedCrossRefGoogle Scholar
  29. Huang TTF and Yanagimachi R (1985) Inner acrosomal membrane of mammalian spermatozoa: its properties and possible function in fertilization. Am J Anat 174: 249–268PubMedCrossRefGoogle Scholar
  30. Jeyendran RS, van der Ven HH, Kennedy WP, Heath E, Perez-Pelaez M, Sobrero AJ and Zaneveld LJD (1985) Acrosomeless sperm. A cause of primary male infertility. Andrologia 17: 31–36PubMedCrossRefGoogle Scholar
  31. Jones R, Brown CR, von Glos KI and Gaunt J (1985) Development of a maturation antigen on the plasma membrane of rat spermatozoa in the epididymis and its fate during fertilization. Exp Cell Res 156: 31–44PubMedCrossRefGoogle Scholar
  32. Koehler JK (1976) Changes in antigenic site distribution on rabbit spermatozoa after incubation in “capacitating” media. Biol Reprod 15: 444–456PubMedCrossRefGoogle Scholar
  33. Koehler JK, de Curtis I, Stenchever MA and Smith D (1983) Freeze-fracture observations on the interaction of human spermatozoa with zona-free hamster oocytes. In: The Sperm Cell, Ed J Andre. Martinus-Nijhoff, The Hague, pp 94–98CrossRefGoogle Scholar
  34. Koehler JK, Smith D and Karp LE (1984) The attachment of acrosome-intact sperm to the surface of zona-free hamster oocytes. Gamete Res 9: 197–205CrossRefGoogle Scholar
  35. Kohn A (1965) Polykaryocytosis induced by Newcastle disease virus in monolayers of animal cells. Virology 26: 228–245PubMedCrossRefGoogle Scholar
  36. Koprowski H and Croce CM (1973) Fusion of somatic and gametic cells with lysolecithin. In: Methods in Cell Biology, Volume VII. Ed DM Prestcott, Acad Press, London, pp 251–260Google Scholar
  37. Lorton SP and First NL (1979) Hyaluronidase docs not disperse the cumulus oophorus surrounding bovine ova. Biol Reprod 21: 301–308PubMedCrossRefGoogle Scholar
  38. Moore HDM and Bedford JM (1978) Ultrastructure of the equatorial segment of hamster spermatozoa during penetration of oocytes. J Ultrastruct Res 62: 110–117PubMedCrossRefGoogle Scholar
  39. Moryan AI, Guay AT and Tulchinsky D (1986) Normal penetration of hamster ova by human spermatozoa with dyskinetic motility. Fert Steril 45: 735–736Google Scholar
  40. Nyormoi O, Coon HG and Sinclair JH (1973) Proliferating hybrid cells formed between rat spermatids and an established line of mouse fibroblasts. J Cell Sci 13: 863–878PubMedGoogle Scholar
  41. Paulsen CA (1983) Another look at the sperm penetration assay. Fert Steril 40: 302–304Google Scholar
  42. Phillips DM and Yanagimachi R (1982) Difference in the manner of association of acrosome-intact and acrosome-reacted hamster spermatozoa with egg microvilli as revealed by scanning electron microscopy. Develop Growth Diff 24: 543–552CrossRefGoogle Scholar
  43. Phillips SG, Phillips DM, Dev VG, Miller DA, van Diggelen OP and Miller OJ (1976) Spontaneous cell hybridization of somatic cells present in sperm suspensions. Exp Cell Res 98: 429–443PubMedCrossRefGoogle Scholar
  44. Podoplelov II, Popivanov RP, Vulchanov VH, Glynsky IA, Kirov KI, Kryukov VG, Zhivkov SM and Nakov LS (1979) The interaction of human spermatozoa with in vitro cultured somatic cells and the potential of this system for the study of fertilizing capacity of human spermatozoa. Biol et Immunologia Reproductionis 1: 12–21Google Scholar
  45. Prasad MRN (1984) The in vitro sperm penetration test: a review. Int J Androl 7: 5–22PubMedCrossRefGoogle Scholar
  46. Quinn P (1979) Failure of human spermatozoa to penetrate zona free mouse and rat ova in vitro. J exp Zool 210: 497–506PubMedCrossRefGoogle Scholar
  47. Rogers BJ (1985) The sperm penetration assay: its usefulness reevaluated. Fert Steril 43: 821–840Google Scholar
  48. Rogers BJ, Ueno M and Yanagimachi R (1981) Fertilization by guinea pig spermatozoa requires potassium ions. Biol Reprod 25: 639–648PubMedCrossRefGoogle Scholar
  49. Russell L, Peterson RN and Freund M (1980) On the presence of bridges linking the inner and outer acrosomal membranes of boar spermatozoa. Anat Rec 198: 449–459PubMedCrossRefGoogle Scholar
  50. Saling PM (1981) Involvement of trypsin-like activity in binding of mouse spermatozoa to zonae pellucidae. Proc Natn Acad Sci US 78: 6231–6235CrossRefGoogle Scholar
  51. Saling PM (1982) Development of the ability to bind to zonae pellucidae during epididymal maturation: reversible immobilization of mouse spermatozoa by lanthanum. Biol Reprod 26: 429–436PubMedCrossRefGoogle Scholar
  52. Schwartz MA and Koehler JK (1979) Alterations in lectin binding to guinea pig spermatoozoa accompanying in utero capacitation and the acrosome reaction. Biol Reprod 21: 1295–1307CrossRefGoogle Scholar
  53. Shalgi R and Phillips DM (1980a) Mechanics of in vitro fertilization in the hamster. Biol Reprod 23: 433–444PubMedCrossRefGoogle Scholar
  54. Shalgi R and Phillips D (1980b) Mechanics of sperm entry in cycling hamsters. J Ultrastruct Res 71: 154–161PubMedCrossRefGoogle Scholar
  55. Siroky J, Spurva V, Kopency V and Tkadlecok L (1979) Acrosomal removal induces features analogous to fertilization during mouse sperm-fusion with somatic cells. J exp Zool 208: 245–254PubMedCrossRefGoogle Scholar
  56. Smith M, Peterson RN and Russell LD (1983) Penetration of zona-free hamster eggs by boar sperm treated with the ionophore A23187 and inhibition of penetration by antiplasma membrane antibodies. J exp Zool 225: 157–160PubMedCrossRefGoogle Scholar
  57. Soupart P, Anderson ML, Albert DH, Coniglio JG and Repp JE (1979) Accumulation, nature, and possible function of the malachite green affinity material in ejaculated human spermatozoa. Fert Steril 32: 450–454Google Scholar
  58. Talbot P and Chacon RS (1982) Ultrastructural observations of binding and membrane fusion between human sperm and zona pellucida-free hamster oocytes. Fert Steril 37: 240–248Google Scholar
  59. Teichmann RJ, Fujimoto M and Yanagimachi R (1972) A previously unrecognized material in mammalian spermatozoa as revealed by malachite green and pyronine. Biol Reprod 7: 73–81Google Scholar
  60. Teichmann RJ, Cummins JM and Takei GH (1974a) The characterization of a malachite green stainable, glutaraldehyde extractable phospholipid in rabbit spermatozoa. Biol Reprod 10: 565–577CrossRefGoogle Scholar
  61. Weissenberg K, Eshkol A, Rudak A and Lunenfield B (1983) Inability of round acrosomeless human spermatozoa to penetrate zona-free hamter ova. Archs Androl 11: 167–169CrossRefGoogle Scholar
  62. Williamson RA, Koehler JK, Dianne Smith W and Karp LE (1984) Entry of immotile spermatozoa into zona-free hamster ova. Gamete Res 10: 319–325CrossRefGoogle Scholar
  63. Wolf DP (1977) Involvement of a trypsin-like activity in sperm penetration of zona-free mouse ova. J exp Zool 199: 149–156PubMedCrossRefGoogle Scholar
  64. Wolf DP and Armstrong PB (1978) Penetration of the zona-free mouse egg by capacitated epididymal sperm: cinematographic observations. Gamete Res 1: 39–46CrossRefGoogle Scholar
  65. Wolf DP, Inoue M and Stark RA (1976) Penetration of zona-free mouse ova. Biol Reprod 15: 213–221PubMedCrossRefGoogle Scholar
  66. Yanagimachi R (1966) Time and process of sperm penetration into hamster ova in vivo and in vitro. J Reprod Fert 11: 359–370CrossRefGoogle Scholar
  67. Yanagimachi R (1972) Penetration of guinea-pig spermatozoa into hamster eggs in vitro. J Reprod Fert 28: 477–480CrossRefGoogle Scholar
  68. Yanagimachi R (1978) Calcium requirement for sperm-egg fusion in mammals. Biol Reprod 19: 949–958PubMedCrossRefGoogle Scholar
  69. Yanagimachi R (1981) Mechanisms of Fertilization in Mammals. In: Fertilization and Embryonic Development In Vitro. Eds L Mastroianni and JD Biggers. Plenum Press, London, pp 81–182Google Scholar
  70. Yanagimachi R (1982) Requirement of extracellular calcium ions for various stages of fertilization and fertilization-related phenomena in the hamster. Gamete Res 5: 323–344CrossRefGoogle Scholar
  71. Yanagimachi R (1984) Zona-free hamster eggs: their use in assessing fertilizing capacity and examining chromosomes of human spermatozoa. Gamete Res 10: 187–232CrossRefGoogle Scholar
  72. Yanagimachi R and Noda YD (1970) Physiological changes in the postnuclear cap region of mammalian spermatozoa: a necessary preliminary to the membrane fusion between sperm and egg cells. J Ultrastruct Res 31: 486–493PubMedCrossRefGoogle Scholar
  73. Yanagimachi R, Nicolson GL, Noda YF and Fujimoto M (1973) Electron microscopic observations of the distribution of acidic and anionic residues on hamster spermatozoa and eggs before and during fertilization. J Ultrastruct Res 43: 344–353PubMedCrossRefGoogle Scholar
  74. Yanagimachi R, Miyashiro LH and Yanagimachi H (1980) Reversible inhibition of sperm-egg fusion in the hamster by low pH. Develop Growth Diff 22: 281–288CrossRefGoogle Scholar
  75. Yanagimachi R, Huang TTF, Fleming AD, Kosower NS and Nicolson GL (1983) Dithiothreitol, a disulphide-reducing agent, inhibits capacitation. acrosome reaction and interaction with eggs by guinea pig spermatozoa. Gamete Res 7: 145–154CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1986

Authors and Affiliations

  • Trevor G. Cooper
    • 1
  1. 1.Max-Planck-Gesellschaft zur Förderung der Wissenschaften e. V.Klinische Forschungsgruppe für Reproduktionsmedizin an der Frauenklinik der Universität MünsterMünsterGermany

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