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Epididymal Secretion of myo-Inositol

  • Trevor G. Cooper

Abstract

Concentrations of inositol decrease along the length of the epididymis of rabbits and bulls (Voglmayr and Amann. 1973) but increase in the rat (Voglmayr, 1974). Scyllo-inositol and glycerophosphoinositol arc also found in epididymal tissue (Seamark et al., 1968) and concentrations of phosphatidylinositol are greater in the head than the tail of the rat epididymis (Scott et al., 1963). Although present in the epididymal lumen in all species, concentrations of free inositol in farm animals (2mM boar: Hartree, 1957; 1–3.7mM ram: Voglmayr et al., 1977; Hinton et al., 1980; 2.5mM bull: Voglmayr and Amann, 1973) are considerably lower than those of rodents (30mM rat; 90 m.M hamster: Hinton et al., 1980) and man (Setchell and Hinton, 1981; Hinton and Setchell, 1981). In man the concentration of myo-inositol in human seminal plasma does not vary in individual fractions of a split ejaculate (Brown-Woodman et al., 1980), so it is thought to arise from all accessory glands, not just the prostate (Lewin and Beer, 1973). Changes along the length of the organ are given in Table 17.

Keywords

Seminal Plasma Cyproterone Acetate Epididymal Spermatozoon Testicular Spermatozoon Human Seminal Plasma 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Akeroyd R and Wirtz KWA (1982) Properties of Phospholipid transfer proteins. In: Membranes and Transport, Vol 1. Ed AN Martonosi, Plenum Press, London, pp 93–98Google Scholar
  2. Berridge MJ (1984) Inositol triphosphate and diacyl glycerol as second messengers. Biochem J 220: 345–360PubMedGoogle Scholar
  3. Berridge MJ and Irvine RF (1984) Inositol triphosphate, a novel second messenger in cellular signal transduction. Nature (Lond) 312: 315–327CrossRefGoogle Scholar
  4. Brown-Woodman PDC. Marley PB. Morris S. Rodger JC and White IG (1980) Origin of glyccrylphosphorylcholinc, inositol, N-acetylaminosugar, and prostaglandins in human seminal plasma and their effects on sperm metabolism. Archs Androl 4: 149–155CrossRefGoogle Scholar
  5. Bruce ICL. Giles CM and Jain SK (1958) Studies in hydrogen-bond formation. Part VII. Reactions between carbohydrates and proteins in water and their relation to transfer of carbohydrates across red-cell membranes. J Chem Soc 1610–1613Google Scholar
  6. Chargraff E, Stewart RN and Magasanik B (1948) Inhibition of mitotic poisoning by meso-inositol. Science 108: 556–558CrossRefGoogle Scholar
  7. Chulavatnatol M (1982) Motility initiation of quiescent spermatozoa from rat caudal epididymis: effects of pH. viscosity, osmolality and inhibitors. Int J Androl 5: 425–436PubMedCrossRefGoogle Scholar
  8. Clegg ED and Foote RH (1973) Phospholipid composition of bovine sperm fractions, seminal plasma and cytoplasmic droplets. J Reprod Pert 34: 379–383CrossRefGoogle Scholar
  9. Clegg ED. Morré DJ and Lunstra DD (1975) Porcine sperm membranes: in vivo phospholipid changes, isolation and electron microscopy. In: The Biology of the Male Gamete. Eds JG Ducked and PA Racey. Biol J Linnaen Soc. Suppl 1. Vol 7 Acad Press, pp 321–335Google Scholar
  10. Cooper TG (1982) Secretion of inositol and glucosc by the perfused rat cauda epididymidis. J Reprod Fert 64: 373–379CrossRefGoogle Scholar
  11. Cooper TG. Yeung CH. Lui W and Yang CZ (1985) Luminal secretion of myo-inositol by the rat epididymis perfused in vitro. J Reprod Fert 74: 135–144CrossRefGoogle Scholar
  12. Dawson RMC (1965) “Phosphatido-peptide”-like complexes formed by the interaction of calcium triphosphoinositol with protein. Biochem J 97: 134–138Google Scholar
  13. Dawson RMC and Scott TW (1964) Phospholipid composition of epididymal spermatozoa prepared by density gradient ccntrifugation. Nature (Lond) 202: 292–293CrossRefGoogle Scholar
  14. Eagle H, Oyama VI, Levy M and Freeman AF. (1957) Myo-inositol as an essential growth factor for normal and malignant human cells in tissue culture. J Biol Chem 226: 191–206PubMedGoogle Scholar
  15. Eisenberg F (1967) D-myoinositol 1-phosphate as product of cyclization of glucose 6-phosphate and substrate for a specific phosphatase in rat testis. J Biol Chem 242: 1375–1382Google Scholar
  16. Eisenberg F and Bolden AH (1964) Reproductive tract as site of synthesis and secretion of inositol in the male rat. Nature (Lond) 202: 599–600Google Scholar
  17. Esbenshade KL and Clegg ED (1980) Surface proteins of ejaculated porcine sperm and sperm incubatcd in the uterus. Biol Reprod 23: 530–537PubMedCrossRefGoogle Scholar
  18. Evans RW and Sctchell BP (1978) Association of exogenous phospholipids with spermatozoa. J Reprod Fert 53: 357–362CrossRefGoogle Scholar
  19. Evans RW and Sctchell BP (1979a) Lipid changcs in boar spermatozoa during epididymal maturation with some observations on the flow and composition of boar rete testis fluid. J Reprod Fert 57: 189–196CrossRefGoogle Scholar
  20. Evans RW and Setchell BO (1979b) Lipid changcs during epididymal maturation in ram spermatozoa collected at different times of the year. J Reprod Fert 57: 197–203CrossRefGoogle Scholar
  21. Hamilton DW (1981) Evidence for α-lactalbumin-like activity in reproductive tract fluids of the male rat. Biol Reprod 25: 385–392PubMedCrossRefGoogle Scholar
  22. Hamilton DW and Olson GE (1976) Effect of carnitine on oxygen uptake and utilization of (U-14C) palmitate by ejaculated bull spermatozoa. J Reprod Fert 46: 195–202CrossRefGoogle Scholar
  23. Hartree EF (1957) Inositol in seminal plasma. Biochem J 66: 131–137PubMedGoogle Scholar
  24. Hasegawa R and Eisenberg F (1981) Selective hormonal control of myoinositol biosynthesis in reproductive organs and liver of the male rat. Proc Natn Acad Sci 78: 4863–4866CrossRefGoogle Scholar
  25. Hawthorne JN (1982) Inositol phospholipids. In: Phospholipids. Eds JN Hawthorne and GB Ansell, Elsevier Biomedical Press. Amsterdam, pp 263–278Google Scholar
  26. Hendrickson HS (1969) Physical properties and interactions of phosphoinositides. Ann NY Acad Sci US 165: 608–676Google Scholar
  27. Hendrickson HS and Ballou CE (1964) Ion exchange chromatography of intact brain phosphoinositides on diethylaminoethyl cellulose by gradient salt elution in a mixed solvent system. J Biol Chem 239: 1369–1373PubMedGoogle Scholar
  28. Hendrickson HS and Fullinglon JG (1965) Stabilities of metal complexes of phospholipids: Ca(II), Mg(II), an Ni(II) complexes of phosphatidylserine and triphosphoinositide. Biochem 4: 1599–1605CrossRefGoogle Scholar
  29. Hendrickson HS and Reinertsen JL (1969) Comparison of metal-binding properties of trans-1,2- cyclohcxanediol diphosphate and deacylated phosphoinositides. Biochem 8: 4855–4858CrossRefGoogle Scholar
  30. Hinton BT and Howards SS (1982) Rat testis and epididymis can transport (3H)3-O-methyl-D-glucose. (3H)inositol and (3H)α-aminoisobutyric acid across its epithelia in vivo. Biol Reprod 27: 1181–1189PubMedCrossRefGoogle Scholar
  31. Hinlon BT and Setchell BP (1981) Micropuncture and microanalytical studies of Rhesus monkey and baboon epididymis and the human ductus deferens. Am J Primatol 1: 251–256CrossRefGoogle Scholar
  32. Hinton BT. White RW and Setchell BP (1980) Concentrations of myo-inositol in the luminal fluid of the mammalian testis and epididymis. J Reprod Fert 58: 395–99CrossRefGoogle Scholar
  33. Hinton BT. Pryor JP. Hirsch AV and Setchell BP (1981) The concentration of some inorganic- ions and organic compounds in luminal fluid of the human ductus deferens. Int J Androl 4: 457–461PubMedCrossRefGoogle Scholar
  34. Jones R and Brown CR (1982) Association of epididymal secretory proteins showing α-lactalbumin-like activity with the plasma membrane of rat spermatozoa. Biochem J 206: 161–164PubMedGoogle Scholar
  35. Kirazov HP and Lagnado JR (1977) Interaction of myo-inositol with brain microtubules. FEBS Letts 81: 173–178CrossRefGoogle Scholar
  36. Lefèvre PG and Davies RI (1951) Active transport into the human erythrocyte: evidence from comparative kinetics and competition among monosaccharides. J Gen Physiol 34: 515–524PubMedCrossRefGoogle Scholar
  37. Lewin LM and Beer R (1973) Prostatic secretion as the source of myo-inositol in human seminal fluid. Fert Steril 24: 666–670Google Scholar
  38. Lewin LM and Sulimovici S (1975) The distribution of radioactive myoinositol in the reproductive tract of the male rat. J Reprod Fert 43: 355–358CrossRefGoogle Scholar
  39. Lewin LM, Nevo Z. Marcus Z. Gabsu A and Nebel L (1978a) Hyaluronidase release from guinea pig spermatozoa as affected by reproductive tract secretions and metabolic inhibitors. Archs Androl 1: 163–167CrossRefGoogle Scholar
  40. Lewin LM. Yannai Y and Kraicer PF (1978b) The distribution of radioactive myo-inositol in the reproductive tract of the male rat. The blood-testis barrier to myoinositol. In: Cyclitols and Phosphoinositides. Eds WW Wells and F Eisenberg, Acad Press. New York, pp 317–322Google Scholar
  41. Lewin LM. Yannai Y and Kraiger P (1979) The effect of cyproterone acetate on myo-inositol uptake and secretion in the reproductive tract of the male rat. Int J Androl 2: 171–181CrossRefGoogle Scholar
  42. Loewus MW. Wright RW. Bondioli KR. Bedgak DL and Karl A (1983) Activity of myo-inositol-1 -phosphate synthase in the epididymal spermatozoa of rams. J Reprod Fert 69: 215–220CrossRefGoogle Scholar
  43. Low MG and Kincade PW (1985) Phosphatidylinositol is the membrane-anchoring domain of the Thy1-glycoprotein. Nature (Lond) 318: 62–64CrossRefGoogle Scholar
  44. Maeda T and Eisenberg F (1980) Purification, structure, and catalytic properties of L-myoinositol-1-phosphate synthase from rat testis. J Biol Chem 255: 8458–8464PubMedGoogle Scholar
  45. Melampy RM and Mason RB (1957) Androgen and the myo-inositol content of male accessory organs of the rat. Proc Soc exp Biol Med 96: 405–408PubMedGoogle Scholar
  46. Michell B (1986) Profusion and confusion. Nature (Lond) 319: 176–177CrossRefGoogle Scholar
  47. Middleton A and Setchell BP (1972) The origin of inositol in the rete testis fluid of the ram. J Reprod Fert 30: 473–475CrossRefGoogle Scholar
  48. Murphy MR, De Lam HH and Chargraff E (1951) Specific inhibition by meso-inositol of the colchicine effect on rat fibroblasts. Exp Cell Res 2: 165–177CrossRefGoogle Scholar
  49. Neill AR and Masters CJ (1971) Incorporation of (U-14C)palmitic acid into the phospholipids of bovine semen. J Reprod Fert 24: 295–297CrossRefGoogle Scholar
  50. Neill AR and Masters CJ (1972) Metabolism of fatty acid by bovine spermatozoa. Biochem J 127: 375–385PubMedGoogle Scholar
  51. Nikolopoulou M, Souck DA and Vary JC (1985) Changes in the lipid content of boar sperm plasma membranes during epididymal maturation. Biochem Biophys Acta 815: 486–498PubMedCrossRefGoogle Scholar
  52. Nixon DA (1970) The effect of sex hormones upon the inositol concentration of the rat seminal vesicle. J Reprod Fert 21: 187–189CrossRefGoogle Scholar
  53. Peterson RN. Russell LD and Hunt WP (1984) Evidence for specific binding of uncapacitated boar spermatozoa to porcine zonae pellucidae in vitro. J exp Zool 231: 137–147PubMedCrossRefGoogle Scholar
  54. Pholpramool C, White RW and Setchell BP (1982) Influence of androgens on inositol secretion and sperm transport in the epididymis of rats. J Reprod Fert 66: 547–553CrossRefGoogle Scholar
  55. Qasba PK. Hewlett IK and Byers S (1983) The presence of the milk protein, α-lactalbumin, and its mRNA in the rat epididymis. Biochem Biophys Res Commun 117: 306–312PubMedCrossRefGoogle Scholar
  56. Roberts ML. Scouten WH and Nyquist SE (1976) Isolation and characterization of the cytoplasmic droplet in the rat. Biol Reprod 14: 421–424PubMedCrossRefGoogle Scholar
  57. Robinson R and Fritz IB (1979) Myoinositol biosynthesis by Sertoli cells and levels of myoinositol biosynthetic enzymes in the testis and epididymis. Can J Biochem 57: 962–967PubMedCrossRefGoogle Scholar
  58. Scott TW and Dawson RMC (1968) Metabolism of phospholipids by spermatozoa and seminal plasma. BioChem J 108: 457–463Google Scholar
  59. Scott TW, Dawson RMC and Rowlands IW (1963) Phospholipid relationships in rat epididymal tissue and spermatozoa. BioChem J 87: 507–512Google Scholar
  60. Scott TW, Voglmayr JK and Setchell BP (1967) Lipid composition and metabolism in testicular and ejaculated ram spermatozoa. BioChem J 102: 456–461Google Scholar
  61. Seamark RF, Tate ME and Smeaton TC (1968) The occurrence of scylloinositol and D-glycerol 1-(L-myoinositol 1-hydrogen phosphate) in the male reproductive tract. J Biol Chem 243: 2424–2428PubMedGoogle Scholar
  62. Setchell BP and Hinton BT (1981)The effects on the spermatozoa of changes in the composition of luminal fluid as it passes along the epididymis. In: The Epididymis: Biology and Pathology. Eds A Clavert and C Bollack. S Karger. Basel. Progr Reprod Biol 8: 58–66Google Scholar
  63. Setchell BP. Dawson RMC and White RW (1968) The high concentration of free myo-inositol in rete testis fluid from rams. J Reprod Fert 17: 219–220CrossRefGoogle Scholar
  64. Setchell BP. Voglmayr JK and Hinks NT (1971) The effect of local heating on the flow and composition of rete testis fluid. J Reprod Fert 24: 81–89CrossRefGoogle Scholar
  65. Slaby F and Bryan J (1976) High uptake of myo-inositol by rat pancratic tissue in vitro stimulates secretion. J Biol Chem 251: 5078–5986PubMedGoogle Scholar
  66. Snider DR and Clegg ED (1975) Alteration of phospholipids in porcine spermatozoa during in vivo uterus and oviduct incubation. J Anim Sci 40: 269–274PubMedGoogle Scholar
  67. Suggett A (1975) Polysaccharides. In: Water. A comprehensive treatise. Volume 4. Aqueous solutions of amphiphilcs and macromoleculcs. Ed F Franks. Plenum Press. London, pp 519–567Google Scholar
  68. Sujarit S. Chaturapanich G and Pholpramool C (1985) Evidence for blood myo-inositol as a source of the epididymal secretion in the perfused cauda epididmidis of the rat. Andrologia 17: 321–326PubMedCrossRefGoogle Scholar
  69. Voglmayr JK (1973) Prostaglandin F concentration in genital tract secretions of dairy bulls. Prostaglandins 4: 673–678PubMedCrossRefGoogle Scholar
  70. Voglmayr JK (1974) Aipha-chlorohydrin-induced changcs in the distribution of free myoinositol and prostaglandin F,. and synthesis of phosphatidylinositol in the rat epididymis. Biol Reprod 11: 593–600PubMedCrossRefGoogle Scholar
  71. Voglmayr JK and Amann RP (1973) The distribution of free myo-inositol in fluid, spermatozoa, and tissues of the bull genital tract and observations on its uptake by the rabbit epididymis. Biol Reprod 8: 504–573PubMedGoogle Scholar
  72. Voglmayr JK and Gandhi JS (1978) Survival of ram testicular spermatozoa in vitro: cffects of glucose, glucose metabolites, rete testis fluid proteins, selected androgens and phospholipids. Theriogenology 9: 463–478CrossRefGoogle Scholar
  73. Voglmayr JK and White IG (1971) Synthesis and metabolism of myoinositol in testicular and ejaculated spermatozoa of the ram. J Reprod Fert 24: 29–37CrossRefGoogle Scholar
  74. Voglmayr JK, Sctchell BP and White IG (1971) The affects of heat on the metabolism and ultrastructure of ram testicular spermatozoa. J Reprod Fert 24: 71–80CrossRefGoogle Scholar
  75. Voglmayr JK. Muslo NA. Saksena SK. Brown-Woodman PDC. Marley PB and White IG (1977) Characteristics of semen collected from the cauda cpididyniidis of conscious ram. J Reprod Fert 49: 245–251CrossRefGoogle Scholar
  76. Webb SJ (1963) Possible role for water and inositol in the structure of nucleoproteins. Nature (Lond) 198: 785–786CrossRefGoogle Scholar
  77. White IG (1954) The effect of some seminal constituents and related substance on diluted mammalian spermatozoa. Aust J Biol Sci 7: 379–3909PubMedGoogle Scholar
  78. Widdas WF (1954) Facilitated transfer of hexoses across the human erythrocyte membrane. J Physiol 125: 163–180PubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1986

Authors and Affiliations

  • Trevor G. Cooper
    • 1
  1. 1.Max-Planck-Gesellschaft zur Förderung der Wissenschaften e. V.Klinische Forschungsgruppe für Reproduktionsmedizin an der Frauenklinik der Universität MünsterMünsterGermany

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